PROBES FOR CLEM

Innovation and application of genetically-encoded probes for correlated live-cell imaging and electron microscopy

 Coordinatore ACADEMISCH ZIEKENHUIS GRONINGEN 

 Organization address address: Hanzeplein 1
city: GRONINGEN
postcode: 9713 GZ

contact info
Titolo: Prof.
Nome: Dick
Cognome: Hoekstra
Email: send email
Telefono: 31-50-3632741
Fax: 31-50-3632728

 Nazionalità Coordinatore Netherlands [NL]
 Totale costo 100˙000 €
 EC contributo 100˙000 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2007-4-3-IRG
 Funding Scheme MC-IRG
 Anno di inizio 2007
 Periodo (anno-mese-giorno) 2007-10-01   -   2011-09-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    ACADEMISCH ZIEKENHUIS GRONINGEN

 Organization address address: Hanzeplein 1
city: GRONINGEN
postcode: 9713 GZ

contact info
Titolo: Prof.
Nome: Dick
Cognome: Hoekstra
Email: send email
Telefono: 31-50-3632741
Fax: 31-50-3632728

NL (GRONINGEN) coordinator 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

possibilities    cell    electron    fluorescent    classes    manner    imaging    protein    genetically    modify    probes    technologies    community    microscope    light    biology    science    recently    organic    endless    localization    specimens    made    palm    proteins    fluorescence    function    encoded    observe    chemistry    live    cells    fixed    probe    localise    correlated    materials    combined    resolution    molecular    photoactivatable    microscopy    tag   

 Obiettivo del progetto (Objective)

'Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. The possibilities are endless: the probes can be used to study proteins in live or fixed specimens; in vitro or in vivo; to study localization or activity, or modify function; and by light and electron microcopy. With the emergence of specialized microscopy units at most universities and research centers, the use of these techniques is well within reach for a broad research community. However, the fluorescent toolbox has become very complex and each tool has specific requirements and pros and cons for different applications. The aim of this project is to develop, validate and implement a probe for combined fluorescence live-cell imaging and electron microscopy of proteins of interest. The combinatorial probe will be based on a genetically-encoded tag consisting of a fluorescent protein (highly suitable for live cell imaging) and horseradish peroxidase to visualize proteins by electron microscopy at high resolution with high quality preservation of the ultrastructure. In addition, we will explore the general application of a recently introduced manner of increasing resolution using light microscopy (photoactivatable localization microscopy; PALM). While the focus of this project proposal is to create probes for general application in cell biology, initially these probes will be implemented in my studies that aim to resolve the role of cell-cell contacts in regulating beta cell proliferation.'

Introduzione (Teaser)

Microscope technology has come a long way, allowing researchers to characterise and localise protein cell behaviour in an unprecedented manner.

Descrizione progetto (Article)

Medicine relies heavily on precise imaging systems to observe protein cells, map their behaviour, diagnose disease and even postulate treatments. In general, electron microscopes that produce an electronically magnified image are used to observe these kinds of cells.

Recent advances in organic chemistry, molecular biology and materials science are enabling improvements in microscope technology. An enterprising European project is creating several new classes of fluorescent probes that can be used for imaging in cell biology. The project, fully funded by the EU, is entitled 'Innovation and application of genetically encoded probes for correlated live-cell imaging and electron microscopy?. It is creating endless possibilities as the probes can be used to study proteins in live or fixed specimens, as well as localise activity of the proteins or even modify their function. The ultimate aim of this project is to develop a probe with two combined technologies, known as fluorescence live-cell imaging and electron microscopy (Clem), which simultaneously target specific proteins.

Increasing the resolution of the imaging is also being investigated, as is light-based microscopy, formally known as photoactivatable localisation microscopy (Palm). The project team has made great strides in its research, successfully introducing molecular biology and cell culture in a traditional electron microscopy lab environment. The combined technologies microscope was developed and tested, resulting in the 'first genuine genetically-encoded tag that allows for live-cell imaging and correlated electron microscopy'. Overall microscopic imaging was improved, and observation of larger areas with the new microscope accomplished. The technology now enables imaging at very fine detail or 'ultrastructural' level, with highly specific targeting. Moreover, since the probe is genuinely 'genetically encoded' and easy to use compared to current fluorescent protein technology, it can be made readily available to the entire cell biology research community.

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REPROGRAMMING (2011)

Molecular mechanisms leading to genome-wide DNA demethylation during epigenetic reprogramming

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OPTMANDROPS (2013)

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SCORE (2012)

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