CYANOIT

Identification and toxic potential of Cyanoprokaryota in the Bulgarian water bodies. Environmental health risks

Coordinatore	UNIVERSITY OF PLOVDIV    more  Organization address address: Tsar Assen Street 24 city: PLOVDIV postcode: 4000 contact info Titolo: Dr. Nome: Rumen Cognome: Mladenov Email: send email Telefono: -261191 Fax: -623820
Nazionalità Coordinatore	Bulgaria [BG]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-2-2-ERG
Funding Scheme	MC-ERG
Anno di inizio	2007
Periodo (anno-mese-giorno)	2007-10-01   -   2011-01-15

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITY OF PLOVDIV  Organization address address: Tsar Assen Street 24 city: PLOVDIV postcode: 4000 contact info Titolo: Dr. Nome: Rumen Cognome: Mladenov Email: send email Telefono: -261191 Fax: -623820	BG (PLOVDIV)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'Blue-green algae (Cyanoprokaryota) are increasingly gaining importance in view of health hazards and ecological risks caused by secondary metabolites with toxic properties named “cyanotoxins”. More than 40 Cyanoprokaryota species are capable to form blooms and produce cyanotoxins. The most common and well-studied producers of cyanotoxins are Microcystis aeruginosa, Aphanisomenon flos-aquae, Anabaena flos-aquae, Cylindrospermopsis raciborskii, Planktothrix agardhii, Lyngbya majuscula, Nodularia and Oscillatoria. Yet, Cyanoprokaryota, often detected in the Bulgarian water bodies, are not studied in toxicological aspects. Therefore, the main goal of this project on the one hand is to identify and characterize the blue-green algae distributed in different Bulgarian freshwater bodies (especially those used for potable and recreational purposes as well as fish-ponds) and on the other hand, to examine their ability to produce intracellular and extracellular toxins. Also, implications on human health and ecological risks caused by harmful freshwater algal blooms will be evaluated and perilous water bodies will be noted and mapped. Correlation between the capacity of toxin production and different environmental factors will be analyzed by ecological models as well as mimicking some environmental factors in in vitro conditions. Collected cyanoprokaryotic samples will be identified by the classical morphological method and if it is necessary by molecular-genetic methods. Determined Cyanoprokaryota species will be adjusted for in vitro culturing and used to study their toxic potential. The toxicity of algal extracts and collected natural water samples will be tested by combination of different in vitro biological assays (enzymatic activity, viability of different cell lines) and the toxins will be identified using ELISA and HPLC methods.'