HINAMOX

"Health Impact of Engineered Metal and Metal Oxide Nanoparticles: Response, Bioimaging and Distribution at Cellular and Body Level"

 Coordinatore ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES 

 Organization address address: PASEO MIRAMON PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 182
city: SAN SEBASTIAN
postcode: 20009

contact info
Titolo: Dr.
Nome: Sergio Enrique
Cognome: Moya
Email: send email
Telefono: +34 943005300
Fax: +34 943005301

 Nazionalità Coordinatore Spain [ES]
 Sito del progetto http://www.hinamox.eu
 Totale costo 2˙927˙577 €
 EC contributo 2˙297˙337 €
 Programma FP7-NMP
Specific Programme "Cooperation": Nanosciences, Nanotechnologies, Materials and new Production Technologies
 Code Call FP7-NMP-2008-SMALL-2
 Funding Scheme CP-FP
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-10-01   -   2012-09-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES

 Organization address address: PASEO MIRAMON PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 182
city: SAN SEBASTIAN
postcode: 20009

contact info
Titolo: Dr.
Nome: Sergio Enrique
Cognome: Moya
Email: send email
Telefono: +34 943005300
Fax: +34 943005301

ES (SAN SEBASTIAN) coordinator 506˙880.33
2    UNIVERSITAET LEIPZIG

 Organization address address: RITTERSTRASSE 26
city: LEIPZIG
postcode: 4109

contact info
Titolo: Prof.
Nome: Edwin
Cognome: Donath
Email: send email
Telefono: -9715996
Fax: -9716041

DE (LEIPZIG) participant 511˙730.40
3    UNIVERSIDAD DE VIGO

 Organization address address: LG CAMPUS LAGOAS MARCOSENDE
city: VIGO PONTEVEDRA
postcode: 36310

contact info
Titolo: Dr.
Nome: Africa
Cognome: González Fernández
Email: send email
Telefono: 34986812626
Fax: -34986812556

ES (VIGO PONTEVEDRA) participant 339˙678.02
4    DET NATIONALE FORSKNINGSCENTER FORARBEJDSMILJO

 Organization address address: LERSO PARKALLE 105
city: KOBENHAVN
postcode: 2100

contact info
Titolo: Dr.
Nome: Soeren Thor
Cognome: Larsen
Email: send email
Telefono: 4539165248
Fax: -4539165201

DK (KOBENHAVN) participant 330˙108.00
5    PLASMACHEM PRODUKTIONS- UND HANDEL GMBH

 Organization address address: RUDOWER CHAUSSEE 29
city: BERLIN
postcode: 12489

contact info
Titolo: Dr.
Nome: Alexei
Cognome: Antipov
Email: send email
Telefono: -63926294
Fax: -63926295

DE (BERLIN) participant 241˙200.00
6    TYOETERVEYSLAITOS

 Organization address address: Topeliuksenkatu 41 a A
city: HELSINKI
postcode: 250

contact info
Titolo: Prof.
Nome: Kai
Cognome: Savolainen
Email: send email
Telefono: +358 30 474 2200
Fax: +358 30 474 2118

FI (HELSINKI) participant 159˙099.60
7    CENTRO DE INVESTIGACION EN QUIMICA APLICADA

 Organization address address: BD ENRIQUE REYNA HERMOSILLO 140
city: SALTILLO
postcode: 25253

contact info
Titolo: Prof.
Nome: Ronald F.
Cognome: Ziolo
Email: send email
Telefono: -11060
Fax: -10797

MX (SALTILLO) participant 110˙499.38
8    Zhejiang University

 Organization address address: ZHE DA ROAD 38
city: HANGZHOU
postcode: 310027

contact info
Titolo: Prof.
Nome: Changyou
Cognome: Gao
Email: send email
Telefono: -87951593
Fax: -87951593

CN (HANGZHOU) participant 98˙141.27
9    EDUCACAO E ENSINO SUPERIOR DO ALTO AVE S.A.

 Organization address address: QUINTA DE MATOS-GERAZ DO MINHO
city: POVOA DE LANHOSO
postcode: 4830-316

contact info
Titolo: Ms.
Nome: Marta
Cognome: Marques
Email: send email
Telefono: +351 253639800
Fax: +351 253639801

PT (POVOA DE LANHOSO) participant 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

spect    traced    oxide    characterization    nanoparticle    techniques    epithelial    labelled    metabolism    oxidative    physiological    toxic    cells    scientists    interaction    nanoparticles    vitro    single    organs    cell    tomography    special    nps    problem    exposure    dose    internalisation    surface    free    intracellular    accumulation    metal    decomposition    pet    ion    ibm    particles    interfere    effect    confocal    impact    signalling    catalytic    microscopy    fluorescently    difficulty    biodistribution    health    size    quantifying    lung    hinamox    immune    emission   

 Obiettivo del progetto (Objective)

'Metal oxide and metal NPs are particularly dangerous for two reasons: their special catalytic activity coming from the properties of their nanointerface may interfere with numerous intracellular biochemical processes and the decomposition of NPs and the ion leakage could heavily interfere with the intracellular free metal ion homeostasis, which is essential for cell metabolism. A very specific problem is the difficulty of localizing and quantifying them in cells. Obtaining dose effect relationships is not simple, because of the unknown amount of material present in affected cells. The following main points will be addressed in this proposal:1) Design and synthesis of metal oxide and metal NPs, which can be traced by SPECT, PET, and fluorescence techniques and the appropriate characterization of these NPs.2) Application of label-free techniques, such as IBM and EM to ensure that the radioactive and fluorescent constituents do not modify the cytological and organismic response by themselves.3) Characterization of the uptake, distribution kinetics and NP release at the level of the organism.4) Study of the interaction of NPs with plasma components forming complexes with NPs and the assessment of their possible impact on the uptake compared with that of bare or capped particles.5) Quantification and localization of metallic NPs in immune competent cells is a key task for the establishment of proper dose-response correlations. A technique applicable with living cells as ultimate control will be IBM, capable of detecting single metal NPs in cells at different depths.6) Development of sophisticated cell physiological approaches focusing on the determination of oxidative activity, cytokine production and adaptive processes concerning signalling pathways beyond standard vitality tests. The research project will indicate toxic levels of various NPs and sub-toxic effects will be investigated by analysing the signalling response of immune cells'

Introduzione (Teaser)

Metal oxide and metal nanoparticles are used in various industrial processes and commonly used products, from sun creams to electronic devices and fuels. A European study investigated the health effects on exposure to a range of nanoparticles.

Descrizione progetto (Article)

Scientists fear that metal and metal oxide nanoparticles may be potentially hazardous due to the special catalytic activity arising as a result of the properties at the nano interface. Additionally, their decomposition may lead to an increase in intracellular ion concentration and interfere with cell metabolism.

A very specific problem in dealing with the toxicological evaluation of nanomaterials, and in particular with metal oxide nanoparticles, is the difficulty of localising and quantifying them in cells and organs. To this end, the EU-funded HINAMOX project synthesised specifically labelled nanoparticles for use in in vitro cell assays to trace their biodistribution.

Radiolabelled metal and metal oxide nanoparticles were generated that could be traced by positron emission tomography (PET) and single photon emission computed tomography (SPECT). Fluorescently labelled particles were also designed and used for uptake studies in vitro.

The intracellular fate of these particles is dependent on their characteristics, surface chemistry, and interaction with proteins and other biologically relevant molecules. To study nanoparticle processing within cells, partners applied confocal Raman microscopy, transmission electron microscopy (TEM), ion beam microscopy (IBM) and confocal laser scanning microscopy.

In vitro culture of nanoparticles with cells revealed that internalisation required at least 12 hours. Uptake was hindered by the presence of protein coronas on the nanoparticle surface and confocal microscopy of fluorescently labelled nanoparticles showed an internalisation pattern that correlated with endosome/lysosome uptake.

Scientists also studied the cytotoxicity, immunological impact and oxidative stress effect of metal nanoparticles on macrophages, alveolar epithelial type two (ATII) cells and lung epithelial cells. This analysis was of high physiological relevance as lung inhalation constitutes the most likely route of nanoparticle exposure.

In vivo biodistribution PET analysis following intravenous administration of nanoparticles unveiled a strong size dependency of the distribution and accumulation of nanoparticles in all organs. However, a negligible accumulation of nanoparticles was observed in the brain irrespective of size.

The results of the HINAMOX study, combined with field measurements to assess nanoparticle emission during powder production, attest to health hazards posed by these novel materials. The outcomes of the study should provide a basis for the formulation of new health and safety procedures to minimise exposure to nanoparticles.

Altri progetti dello stesso programma (FP7-NMP)

MEM4WIN (2012)

"Ultra thin glass membranes for advanced, adjustable and affordable quadruple glazing windows for zero-energy buildings"

Read More  

FIT4U (2009)

Framework of Integrated Technologies for User Centred Products

Read More  

HIPPOCAMP (2013)

High-power Impulse Plasma Process Operations for the Creation of Advanced Metallic Parts

Read More