AMBIOCAS

AMINE SYNTHESIS THROUGH BIOCATALYTIC CASCADES

 Coordinatore THE UNIVERSITY OF MANCHESTER 

 Organization address address: OXFORD ROAD
city: MANCHESTER
postcode: M13 9PL

contact info
Titolo: Ms.
Nome: Liz
Cognome: Fay
Email: send email
Telefono: +44 161 275 7114
Fax: +44 161 275 2445

 Nazionalità Coordinatore United Kingdom [UK]
 Sito del progetto http://www.ambiocas.eu/
 Totale costo 3˙466˙757 €
 EC contributo 2˙524˙792 €
 Programma FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology
 Code Call FP7-KBBE-2009-3
 Funding Scheme CP-FP
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-01-01   -   2013-06-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF MANCHESTER

 Organization address address: OXFORD ROAD
city: MANCHESTER
postcode: M13 9PL

contact info
Titolo: Ms.
Nome: Liz
Cognome: Fay
Email: send email
Telefono: +44 161 275 7114
Fax: +44 161 275 2445

UK (MANCHESTER) coordinator 589˙350.00
2    DANMARKS TEKNISKE UNIVERSITET

 Organization address address: Anker Engelundsvej 1, Building 101A
city: KONGENS LYNGBY
postcode: 2800

contact info
Titolo: Prof.
Nome: John
Cognome: Woodley
Email: send email
Telefono: +45 4525 2885
Fax: +45 4593 2906

DK (KONGENS LYNGBY) participant 697˙500.00
3    UNIVERSITAET GRAZ

 Organization address address: UNIVERSITAETSPLATZ 3
city: GRAZ
postcode: 8010

contact info
Titolo: Prof.
Nome: Wolfgang
Cognome: Kroutil
Email: send email
Telefono: +43 316 380 5350
Fax: +43 316 380 9840

AT (GRAZ) participant 528˙240.00
4    RIJKSUNIVERSITEIT GRONINGEN

 Organization address address: Broerstraat 5
city: GRONINGEN
postcode: 9712CP

contact info
Titolo: Dr.
Nome: Jan
Cognome: Poutsma
Email: send email
Telefono: +31 50 3634142
Fax: +31 50 36348500

NL (GRONINGEN) participant 328˙192.00
5    CLEA TECHNOLOGIES BV

 Organization address address: DELFTECHPARK 34
city: DELFT
postcode: 2628 HX

contact info
Titolo: Mr.
Nome: Ivo
Cognome: Van Der Laan
Email: send email
Telefono: +31 15 2787138
Fax: +31 15 2782607

NL (DELFT) participant 256˙604.00
6    EVONIK INDUSTRIES AG

 Organization address address: RELLINGHAUSER STRASSE 1-11
city: ESSEN
postcode: 45128

contact info
Nome: Patrick
Cognome: Sittig
Email: send email
Telefono: +49 6181 59 4785

DE (ESSEN) participant 64˙250.00
7    CHIROTECH TECHNOLOGY LIMITED

 Organization address address: MILTON ROAD 410 Cambridge Science Park -
city: CAMBRIDGE
postcode: CB4 0PE

contact info
Titolo: Mr.
Nome: Srinivas
Cognome: Dronamraju
Email: send email
Telefono: 441924000000

UK (CAMBRIDGE) participant 60˙656.00
8    EVONIK DEGUSSA GmbH

 Organization address address: Rellinghauser Str. 1-11
city: Essen
postcode: 45128

contact info
Titolo: Dr.
Nome: Jan
Cognome: Pfeffer
Email: send email
Telefono: +49 2365 49 5457
Fax: +49 2365 49 7110

DE (Essen) participant 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

transaminases    synthesise    polymers    acid    co    cleaner    functionality    chemical    ambiocas    intermediates    amine    extra    overcome    aminocaproic    enzymes    functional    designed    cascades    industrial    transaminase    synthesis    amines    enzyme    enzymatic    reaction    cascade    steps    significant    conventional    energy   

 Obiettivo del progetto (Objective)

'This project intends to engineer transaminase libraries that will be applied as the main enzymatic technology to deliver the amine functionality in the commercially valuable products of both chiral and bulk amine targets. These enzymes will be used in enzymatic cascades where simple starting materials are converted into the required intermediates for transamination or further enzymatic steps will be used to remove products from the transaminase reaction which will add value by extra functionality. This will also be supported by the development of enzymatic cascades to deliver efficient co-factor recycling and achieve the high conversions required for industrial use. A high throughput screening method based on a further enzymatic cascade will be developed. Engineering solutions will be used to overcome obstacles associated with the implementation of this core technology on a larger scale and integrate the use of other enzymes into the synthetic pathway to allow multi-step, multi-enzyme cascades to be used to deliver complex multi-functional amine products and processes. The industrial partner will target the development of enzymes from the project for specific application into their new products range. Life cycle analysis and environmental impact analysis will compare the final methods with conventional chemical synthesis and allow advantages to be objectively defined.'

Introduzione (Teaser)

There is an ongoing quest for safer and cleaner chemical methods to synthesise various compounds. European researchers designed enzymatic cascades that could complement or replace classical chemistry in the development of amine-containing products.

Descrizione progetto (Article)

Amines are important chemical intermediates that are extensively exploited in the production of polymers, pharmaceuticals and other chemicals. A very popular example is 6-aminocaproic acid, a compound used in the production of polyamide polymers in applications such as textiles, floor coverings or other nylon-type products.

The conventional chemical synthesis route entails the use of phenol and has a significant energy and waste pattern. To resolve this, scientists on the EU-funded 'Amine synthesis through biocatalytic cascades' (http://www.ambiocas.eu (AMBIOCAS)) project proposed to synthesise amines by exploiting the catalytic activity of transaminase enzymes.

To this end, various mutant transaminases were genetically engineered and a library was created. These mutants were designed based on the elucidation of the three-dimensional structures of several transaminases by protein X-ray crystallography. The long-term plan was to use these enzymes in biotechnology applications to synthesise amine functional groups in various commercial products.

Following the identification of the transaminase enzymes that would catalyse the conversion to amines, enzymatic cascades were designed for obtaining extra chemical functionality. A series of issues had to be overcome first, including the unfavourable thermodynamic equilibrium of converting a ketone to an amine. Detailed analysis of the kinetics and energy parameters of the reaction led to the optimisation of reaction conditions and enzyme properties. A key innovative step in the AMBIOCAS enzymatic cascade was the co-factor regeneration during the multi-step process without the need for intermediate isolation. Overall, this simplified the synthesis of 6-aminocaproic acid in six enzymatic steps.

Given the high activity and selectivity of enzymes, the AMBIOCAS enzyme-based method holds significant economic benefits with the cleaner synthesis of desired products. This technology will limit the exposure to toxic, flammable and explosive hazards commonly encountered in the chemical industry.

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