INFBIOSYS

THE ROLE OF BACTERIAL MULTIGENE FAMILIES IN INFECTION: IDENTIFICATION OF HOST TARGET NETWORKS BY HIGH-THROUGHPUT PROTEIN INTERACTOMICS

 Coordinatore THE UNIVERSITY OF EDINBURGH 

 Organization address address: OLD COLLEGE, SOUTH BRIDGE
city: EDINBURGH
postcode: EH8 9YL

contact info
Titolo: Ms.
Nome: Angela
Cognome: Noble
Email: send email
Telefono: +44 131 650 9024
Fax: +44 131 651 4028

 Nazionalità Coordinatore United Kingdom [UK]
 Totale costo 173˙240 €
 EC contributo 173˙240 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2009-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-07-01   -   2012-06-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF EDINBURGH

 Organization address address: OLD COLLEGE, SOUTH BRIDGE
city: EDINBURGH
postcode: EH8 9YL

contact info
Titolo: Ms.
Nome: Angela
Cognome: Noble
Email: send email
Telefono: +44 131 650 9024
Fax: +44 131 651 4028

UK (EDINBURGH) coordinator 173˙240.80

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

equi    multigene    family    bacterial    host    surface    pathogenic    virulence    mtb    pathogen    related    proteins    families    interaction    pathways    pathogenesis   

 Obiettivo del progetto (Objective)

'The genomes of pathogenic bacteria often contain multiple copies of genes with similar sequence. The encoded proteins are generally secreted or associated with the bacterial surface and are thus likely to be involved in the host-pathogen interaction. However, the functions of these multigene families in pathogenesis remain largely unknown. We will investigate the role of bacterial multigene family proteins in the host-pathogen interaction taking a Systems Biology-based approach. We will focus on two model pathogen-specific virulence-related proteins families: the Inl (internalin) proteins from the gram-positive genus Listeria, and the Vap (virulence-associated) proteins from Rhodococcus equi, a pathogenic actinomycete related to Mycobacterium tuberculosis (MTB). We will also investigate the Mce and PE/PPE proteins, which are distributed among several pathogenic actinobacteria including R. equi and MTB. Different members of these multigene families are found in genotypes or species differing in virulence and pathogenic tropism. Our hypothesis is that the specific multigene complements carried by the different “pathovars” contribute to their distinct pathogenic properties. We will address the research question by systematically identifying binding partners to the investigated proteins and relevant domains thereof by interaction proteomics using high-throughput yeast two-hybrid (Y2H) screens with large host (mammalian) cDNA prey libraries. We will then apply bioinformatics tools and network analysis to define the host subcellular compartments and functional networks and pathways targeted by the bacterial multigene family proteins, and the global contribution of the identified interactions to pathogenesis. Microbial surface-associated and excreted proteins are at the forefront of the host-pathogen interplay, mediating adherence/colonisation, subverting host pathways, or modulating innate or acquired immunity.'

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