Autonomous DNA Evolution in a Molecule Trap


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 Nazionalità Coordinatore Germany [DE]
 Totale costo 1˙487˙827 €
 EC contributo 1˙487˙827 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2010-StG_20091028
 Funding Scheme ERC-SG
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-08-01   -   2015-07-31


# participant  country  role  EC contrib. [€] 

 Organization address address: GESCHWISTER SCHOLL PLATZ 1
postcode: 80539

contact info
Titolo: Ms.
Nome: Monika
Cognome: Bernhardt
Email: send email
Telefono: +49 89 21803449
Fax: +49 89 2180 2985

DE (MUENCHEN) hostInstitution 1˙487˙827.00

 Organization address address: GESCHWISTER SCHOLL PLATZ 1
postcode: 80539

contact info
Titolo: Prof.
Nome: Dieter
Cognome: Braun
Email: send email
Telefono: +49 89 2180 2317
Fax: +49 89 2180 16558

DE (MUENCHEN) hostInstitution 1˙487˙827.00


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

replicate    dna    hairpins    trna    create    continuous    evolution    protein    trap    molecular    degradation    basic    replication    biotechnological    questions    convective    entropic    drive    nonequilibrium    will    trapping    molecule    uv    thermal   

 Obiettivo del progetto (Objective)

'How can we create molecular life in the lab? That is, can we drive evolvable DNA/RNA-machines under a simple nonequilibrium setting? We will trigger basic forms of autonomous Darwinian evolution by implementing replication, mutation and selection on the molecular level in a single micro-chamber? We will explore protein-free replication schemes to tackle the Eigen-Paradox of replication and translation under archaic nonequilibrium settings. The conditions mimic thermal gradients in porous rock near hydrothermal vents on the early earth. We are in a unique position to pursue these questions due to our previous inventions of convective replication, optothermal molecule traps and light driven microfluidics. Four interconnected strategies are pursued ranging from basic replication using tRNA-like hairpins, entropic cooling or UV degradation down to protein-based DNA evolution in a trap, all with biotechnological applications. The approach is risky, however very interesting physics and biology on the way. We will: (i) Replicate DNA with continuous, convective PCR in the selection of a thermal molecule trap (ii) Replicate sequences with metastable, tRNA-like hairpins exponentially (iii) Build DNA complexes by structure-selective trapping to replicate by entropic decay (iv) Drive replication by Laser-based UV degradation Both replication and trapping are exponential processes, yielding in combination a highly nonlinear dynamics. We proceed along publishable steps and implement highly efficient modes of continuous molecular evolution. As shown in the past, we will create biotechnological applications from basic scientific questions (see our NanoTemper Startup). The starting grant will allow us to compete with Jack Szostak who very recently picked up our approach [JACS 131, 9628 (2009)].'

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