MMEP

Understanding the molecular mechanism of enzymatic processes by a combination of NMR spectroscopy and molecular dynamics simulations

 Coordinatore THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE 

 Organization address address: The Old Schools, Trinity Lane
city: CAMBRIDGE
postcode: CB2 1TN

contact info
Titolo: Ms.
Nome: Renata
Cognome: Schaeffer
Email: send email
Telefono: +44 1223 333543
Fax: +44 1223 332988

 Nazionalità Coordinatore United Kingdom [UK]
 Totale costo 200˙049 €
 EC contributo 200˙049 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2010-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-02-01   -   2014-01-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE

 Organization address address: The Old Schools, Trinity Lane
city: CAMBRIDGE
postcode: CB2 1TN

contact info
Titolo: Ms.
Nome: Renata
Cognome: Schaeffer
Email: send email
Telefono: +44 1223 333543
Fax: +44 1223 332988

UK (CAMBRIDGE) coordinator 200˙049.60

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

structural    diseases    sampling    shifts    data    incorporated    simulations    enzymes    cypa    input    successfully    disease    rates    magnetic    chemical    definition    action    proteins    atom    team    spectroscopy    molecular    experimental    resonance    involving    pagp    enzyme    reactions    dynamics    reaction    protein    measured    magnitude    substrate    orders    mmep    atomic    nuclear    human    cyclophilin   

 Obiettivo del progetto (Objective)

'Essentially all the biochemical processes taking place in living organisms require the intervention of enzymes, which are proteins capable of increasing the rates of chemical reactions by up to 15 orders of magnitude. A detailed definition of the molecular basis of the action of enzymes will not only represent a key advance in our understanding of the fundamental principles of macromolecular behavior, but also provide new opportunities for the rational development of effective treatments for human disease, and for the control of chemical processes in biotechnology. In this application, we propose to characterize in detail the enzymatic processes of two important proteins, cyclophilin A and PagP, by using an interdisciplinary approach in which experimental measurements are incorporated as structural restraints in molecular dynamics simulations. The innovative aspect of this project is the use of chemical shifts, which are the parameters that can be measured most readily and accurately in nuclear magnetic resonance spectroscopy, for protein structure determination. This approach, which has been pioneered in the host lab, enables to determine with high accuracy the structures of proteins in states that are not easily accessible through other types of measurements. We will therefore have the opportunity to gain access to conformations that are invisible by standard methods of structural biology, and in this way to clarify the mechanism of action of two important enzymes.'

Introduzione (Teaser)

Enzymes can increase rates of reaction by 15 orders of magnitude, a thousand trillion times faster! EU research has looked at the dynamics of enzyme behaviour in a reaction at atomic level.

Descrizione progetto (Article)

Involved in all chemical reactions in the body, enzymes are crucial for optimal health and can be used in many industrial processes mimicking natural systems. The MMEP project has developed a detailed definition of action for two important enzymes, cyclophilin A (CypA) and PagP. CypA in particular is a key player in human disease. It regulates protein folding and trafficking and is secreted in response to inflammatory stimuli.

Researchers incorporated experimental measurements in molecular dynamics simulations. The team used a novel approach involving chemical shifts, changes in resonant frequencies of the nuclei of the atoms. These were measured using nuclear magnetic resonance spectroscopy.

Input of experimental data resulted in effective corrections of the force field so the simulation is in better agreement with the experimental data. The scientists also integrated advanced sampling methods to alleviate the problem of conformational sampling as the molecules change shape to effect changes in the substrate.

MMEP has unveiled the details of how CypA works at the atomic level when the enzyme successfully substitutes an atom in the substrate. Using this model as a base, the team has designed a striking test involving a single atom substitution that is enough to regulate the turnover of the reaction.

Project work has successfully outlined the sub-molecular dynamics of the enzyme CypA. Ubiquitous in cell management, CypA has an input into diseases as diverse as cardiovascular diseases, viral infections, neurodegeneration, cancer, rheumatoid arthritis, sepsis, asthma and periodontitis.

It is likely that elucidating the role of CyPA will provide a better understanding of the molecular mechanisms underlying these diseases and will help develop novel pharmacological therapies.

Altri progetti dello stesso programma (FP7-PEOPLE)

CARA (2010)

CARA POSTDOCTORAL MOBILITY FELLOWSHIPS IN THE HUMANITIES AND SOCIAL SCIENCES

Read More  

CARMESH (2009)

Ubiquitous Wireless Mesh Networks for Next-Generation Personal Digital Automotive Services

Read More  

AVIAN EVOLUTION (2010)

Avian brain evolution and higher level phylogenetics of modern birds

Read More