PARAFLUO

Parallel fluorescence spectroscopy tools for micro and nano-analytical applications down to single biomolecules

 Coordinatore MICRO PHOTON DEVICES S.R.L. 

 Organization address address: Via Stradivari 4
city: BOLZANO
postcode: 39100

contact info
Titolo: Dr.
Nome: Roberto
Cognome: Biasi
Email: send email
Telefono: +39 0471 051 212
Fax: +39 0471 501 524

 Nazionalità Coordinatore Italy [IT]
 Sito del progetto http://www.parafluo.com
 Totale costo 1˙463˙749 €
 EC contributo 1˙133˙000 €
 Programma FP7-SME
Specific Programme "Capacities": Research for the benefit of SMEs
 Code Call FP7-SME-2008-1
 Funding Scheme BSG-SME
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-06-01   -   2011-08-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    MICRO PHOTON DEVICES S.R.L.

 Organization address address: Via Stradivari 4
city: BOLZANO
postcode: 39100

contact info
Titolo: Dr.
Nome: Roberto
Cognome: Biasi
Email: send email
Telefono: +39 0471 051 212
Fax: +39 0471 501 524

IT (BOLZANO) coordinator 543˙030.00
2    PicoQuant GmbH

 Organization address address: Rudower Chaussee 29
city: Berlin
postcode: D-12489

contact info
Titolo: Dr.
Nome: Rainer
Cognome: Erdmann
Email: send email
Telefono: +49 30 63926560
Fax: +49 30 6392 6561

DE (Berlin) participant 417˙009.00
3    Helia Photonics Ltd

 Organization address address: Rosebank Park 2
city: Livingston
postcode: EH54 7EJ

contact info
Titolo: Dr.
Nome: Caspar
Cognome: Clark
Email: send email
Telefono: +44 1506 414800
Fax: +44 1506 414070

UK (Livingston) participant 110˙251.00
4    CF CONSULTING FINANZIAMENTI UNIONE EUROPEA SRL

 Organization address address: Via Giuseppe Mussi 1
city: MILANO
postcode: 20154

contact info
Titolo: Mrs.
Nome: Carla
Cognome: Finocchiaro
Email: send email
Telefono: +39 02 310833 1
Fax: +39 02 33614064

IT (MILANO) participant 31˙000.00
5    CONSIGLIO NAZIONALE DELLE RICERCHE

 Organization address address: Piazzale Aldo Moro 7
city: ROMA
postcode: 185

contact info
Titolo: Dr.
Nome: Piera
Cognome: Maccagnani
Email: send email
Telefono: +39 051 6399104

IT (ROMA) participant 6˙431.00
6    POLITECNICO DI MILANO

 Organization address address: PIAZZA LEONARDO DA VINCI 32
city: MILANO
postcode: 20133

contact info
Titolo: Prof.
Nome: Massimo
Cognome: Ghioni
Email: send email
Telefono: +39 02 23996093

IT (MILANO) participant 6˙380.00
7    Fachhochschule Brandenburg

 Organization address address: Magdeburger Str. 50
city: Brandenburg
postcode: 14770

contact info
Titolo: Prof.
Nome: Gerald
Cognome: Kell
Email: send email
Telefono: +49 3381 355422
Fax: +49 3381355199

DE (Brandenburg) participant 6˙300.00
8    GEORG-AUGUST-UNIVERSITAET GOETTINGEN STIFTUNG OEFFENTLICHEN RECHTS

 Organization address address: WILHELMSPLATZ 1
city: GOTTINGEN
postcode: 37073

contact info
Titolo: Prof.
Nome: Joerg
Cognome: Enderlein
Email: send email
Telefono: +49 7071 2976911
Fax: +49 551 397720

DE (GOTTINGEN) participant 6˙300.00
9    HERIOT-WATT UNIVERSITY

 Organization address address: Riccarton
city: EDINBURGH
postcode: EH14 4AS

contact info
Titolo: Prof.
Nome: Gerald
Cognome: Buller
Email: send email
Telefono: +44 131 451 3069

UK (EDINBURGH) participant 6˙299.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

correlated    imaging    diode    sme    spectrally    array    setup    counting    time    interactions    technique    detector    physiology    confocal    cellular    active    optoelectronic    resolved    microscopy    biological    onto    flim    fluorescence    base    instrumentation    techniques    parafluo    medical    single    market    micro    labelled    silicon    photon    lifetime    tissues    spectral    employed    microscope    avalanche    emission    analytical   

 Obiettivo del progetto (Objective)

'Analytical methods based on fluorescence measurements are widely employed for investigating biological process at cellular level. A modern technique is fluorescence-lifetime imaging microscopy (FLIM), where a map is obtained of the fluorescent emission lifetime versus position in a cell. The objective of project PARAFLUO is an innovative instrumentation system that will enhance and extend the usefulness of FLIM, making possible to obtain simultaneously FLIM data separately for the various spectral components of the emission. There is wide consensus among experimenters that this spectrally resolved technique (called sFLIM) will support a better understanding of the biological processes involved. Such understanding is paramount for the (patho)physiology of tissues and organisms and gives a base for gaining a better insight in key medical issues, such as the origin and growth mechanisms of tumors. The optoelectronic instrumentation developed will be useful also for other market objectives, such as simultaneous multi-spectral profiling of objects by laser detection and ranging (LADAR) techniques. The developments envisaged are essentially: (a) a photon-counting array detector based on the silicon single-photon avalanche diode (SPAD) technology; (b) a new micro-lens system for focusing light onto the detector and (c) an ASIC based multichannel time correlated single photon counting (TCSPC) system, integrated with an optoelectronic setup in a confocal microscope. The base of the PARAFLUO consortium is given by three SME’s; each one having a consolidated technical know-how and an active presence in the market over one of the quoted scientific-technical (S/T) areas. Five RTD performers have been selected primarily because of their high international standard in these areas; furthermore, each of them has experience of active collaboration with the SME directly concerned by the specific S/T work. A professional partner supports the coordinator and ensures timely and efficient exchange of materials and information in the project.'

Introduzione (Teaser)

Analytical techniques that provide information on the interactions among biomolecules could help discover the micro- and nanoworlds of biological processes.

Descrizione progetto (Article)

The majority of biological processes entail a complex interplay among different macromolecules such as proteins, sugars, lipids and nucleotides. To understand and monitor these processes and their impact on tissue physiology, scientists have employed fluorescence imaging techniques.

Fluorescence-lifetime imaging microscopy (FLIM) is a technique that relies on the decay of fluorescence measuring the changes either of the spectral intensity or of the lifetime of the fluorescence. As a result, it could be used to discriminate among different environments and to provide information about the labelled molecules and their interactions.

Based on this, the EU-funded http://www.parafluo.com/ (PARAFLUO) project aimed to address the rising quest for analytical instrumentation suitable in this field. To this end, project partners set out to develop optoelectronic instrumentation capable of carrying out spectrally resolved FLIM.

Project activities included the design of a photon-counting array detector based on the silicon single-photon avalanche diode technology. Additionally, a microlens system was fabricated and integrated onto the detector alongside a multi-channel time-correlated single-photon counting system. The whole setup was attached to a confocal microscope to facilitate visualisation of labelled cells or tissues.

The advanced optoelectronic instrumentation generated by PARAFLUO small businesses will enhance their competitiveness in the European market. From a research viewpoint, it will help to unravel the molecular machines that orchestrate cellular processes and to gain better insight into key medical issues.

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