PRE-MRNA SPLICING

Coordinated regulation of transcription and pre-mRNA splicing at gene promoters

 Coordinatore MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. 

 Organization address address: Hofgartenstrasse 8
city: MUENCHEN
postcode: 80539

contact info
Titolo: Dr.
Nome: Birgit
Cognome: Knepper-Nicolai
Email: send email
Telefono: +49 3512102772
Fax: +49 3512101089

 Nazionalità Coordinatore Germany [DE]
 Totale costo 162˙742 €
 EC contributo 162˙742 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2010-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2011
 Periodo (anno-mese-giorno) 2011-05-01   -   2013-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

 Organization address address: Hofgartenstrasse 8
city: MUENCHEN
postcode: 80539

contact info
Titolo: Dr.
Nome: Birgit
Cognome: Knepper-Nicolai
Email: send email
Telefono: +49 3512102772
Fax: +49 3512101089

DE (MUENCHEN) coordinator 162˙742.40

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

mrna    model    gene    bac    chip    lines    coupling    genes    protein    alternative    pre    recruitment    outcome    splicing    cell    genome    sr    family    spliceosome    promoter    proteins   

 Obiettivo del progetto (Objective)

'Accurate pre-mRNA splicing is a fundamental step for gene expression. SR proteins, a protein family of splicing regulators, play an essential role in the process by defining intron-exon boundaries and recruiting the spliceosome to carry out splicing. Pre-mRNA splicing occurs co-transcriptionally and it is now emerging that promoter identity modulates alternative splicing, possibly through the recruitment of SR proteins or through kinetic coupling by altering Pol II elongation rates. The aim of the present proposal is to investigate the relationship between promoter elements, differential SR protein recruitment to genes and splicing outcome. The genome-wide recruitment of SR proteins to promoter elements will be investigated resorting to innovative approaches. SR proteins tagged with LAP-tag and expressed in 'BAC cell lines' will be isolated with associated DNA sequences (ChIP) which will then be used to interrogate a genome-wide promoter microarray (ChIP-on-CHIP). This will reveal similarities and differences in recruitment to genes and gene regions among SR protein family. To test how promoter elements influence spliceosome assembly and splicing outcome, model-genes containing alternative and constitutively spliced exons will be created. The focus will be on genes relevant for neuronal differentiation. Model genes will be introduced in cells already expressing individual SR proteins BACs, creating 'Double-BAC cell lines'. The results expected from this project will provide a global view of SR protein role in regulating and coupling transcription and pre-mRNA splicing. Elucidating these regulatory mechanisms is key for the understanding of genomic and proteomic diversity.'

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