BIOPHYSRETRAIN

Biophysics of Reverse Transcription and its Inhibition

Coordinatore	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY    more  Organization address address: TECHNION CITY - SENATE BUILDING city: HAIFA postcode: 32000 contact info Titolo: Mr. Nome: Mark Cognome: Davison Email: send email Telefono: +972 4 829 3097 Fax: +972 4 823 2958
Nazionalità Coordinatore	Israel [IL]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-CIG
Funding Scheme	MC-CIG
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-09-01   -   2015-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY  Organization address address: TECHNION CITY - SENATE BUILDING city: HAIFA postcode: 32000 contact info Titolo: Mr. Nome: Mark Cognome: Davison Email: send email Telefono: +972 4 829 3097 Fax: +972 4 823 2958	IL (HAIFA)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'As the major target for therapy, Reverse Transcriptase (RT), and in particular the RT from the HIV virus, has been the subject of extensive research. However, despite an ever-increasing amount of information from traditional “bulk” studies, the detailed mechanisms of polymerization are still not completely understood. This is due, partially, to the stochastic nature of individual enzymes, which makes it impossible to follow more than one or two individual steps of polymerization using ensemble methods. I propose here to conduct a single-molecule, mechanistic study of HIV RT, by developing a high-resolution optical-tweezers, capable of resolving the individual polymerization and translocation steps of RT at a broad range of forces and nucleotides concentrations. Characterizing the dwell-time distribution at different experimental condition will allow us to study the complete mechano-chemical cycle of RT, its regulation by the template’s backbone-composition, structure and sequence, its interaction with additional viral and host factors, the molecular basis of inhibition by known inhibitors and the mechanism of resistance acquired through different mutations.'