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NChIP SIGNED

Chromatin dynamics during DNA replication

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

NChIP project word cloud

Explore the words cloud of the NChIP project. It provides you a very rough idea of what is the project "NChIP" about.

environmental fundamental newly site majority maintenance implying potentially smaller activation re reassembly dna split gene differentiation tetramers programs synthesized footprint establishment positioning histone h4 blocs protein genomic driver maternal strands h3 survive 400bp stay phenomena kinetics strand chromatin spread distribute daughter demonstrated fold lagging inherited regulators renewal marks proteins measuring genome previously bulk assembly replication cellular equally movements later posttranslational transcriptional differences locus few integrity 1kb facts histones unmarked domains predispositions epigenetic original perturbations disease cerevisiae shown binding theory dynamics nucleosomes nucleosome thought inheritance components heritable cancer throughput se diluted encoded prerequisite accepted effect influence

Project "NChIP" data sheet

The following table provides information about the project.

Coordinator	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	1˙984˙677 €
EC max contribution	1˙984˙677 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2014-CoG
Funding Scheme	ERC-COG
Starting year	2015
Duration (year-month-day)	from 2015-06-01 to 2020-05-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	1˙984˙677.00

Map

Project objective

Chromatin assembly is a fundamental cellular process necessary for the maintenance of genome integrity and transcriptional programs. Understanding the effect of DNA replication on histone protein dynamics is also a prerequisite for understanding the role of chromatin in epigenetic inheritance. Epigenetic phenomena are thought to influence cellular differentiation and cancer formation, as well as the impact of environmental factors on early development and later predispositions to disease. While epigenetic inheritance of chromatin components is, in theory, accepted as the driver of such phenomena, chromatin state inheritance per se has only been demonstrated for a few specific cases. Not much is known about histone “inheritance” beyond the facts that bulk maternal histones distribute equally among the daughter strands and are diluted two-fold after replication with newly synthesized “unmarked” histones, and that the majority of H3/H4 tetramers do not split before reassembly. We have shown previously that maternal nucleosomes stay on average within 400bp of their original binding site, implying that any potentially heritable chromatin encoded information, has to be inherited in ~1kb blocs, as smaller nucleosome domains would rapidly be diluted by new nucleosomes. I propose to develop high throughput systems for directly measuring movements of histones and chromatin regulators during genomic replication in S.cerevisiae to determine, how chromatin states survive the perturbations associated with replication. We will determine locus specific differences in the spread of maternal nucleosomes after replication, the effects of leading and lagging strand replication on nucleosome positioning and maternal nucleosome distribution, the renewal dynamics of posttranslational histone marks and chromatin binding proteins, and the kinetics of chromatin footprint re-establishment and gene (re)activation.

Publications

List of publications.
year	authors and title	journal	last update
2019	Rahima Ziane, Alain Camasses, Marta Radman-Livaja Mechanics of DNA Replication and Transcription Guide the Asymmetric Distribution of RNAPol2 and New Nucleosomes on Replicated Daughter Genomes published pages: , ISSN: , DOI: 10.1101/553669	biorxiv	2020-02-05
2019	Hrvoje Galic, Pauline Vasseur,Marta Radman-Livaja The budding yeast heterochromatic SIR complex resets upon exit from stationary phase published pages: , ISSN: , DOI: 10.1101/603613	biorxiv	2020-02-05
2016	Pauline Vasseur, Saphia Tonazzini, Rahima Ziane, Alain Camasses, Oliver J. Rando, Marta Radman-Livaja Dynamics of Nucleosome Positioning Maturation following Genomic Replication published pages: 2651-2665, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.07.083	Cell Reports 16/10	2019-06-06

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