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MEMBRANEFUSION SIGNED

Structure and mechanism of viral and cellular membrane fusion machineries

Total Cost €

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EC-Contrib. €

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Partnership

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 MEMBRANEFUSION project word cloud

Explore the words cloud of the MEMBRANEFUSION project. It provides you a very rough idea of what is the project "MEMBRANEFUSION" about.

fluorescence    regulatory    trafficking    mechanistic    proteins    cryo    assembly    membranes    fuse    core    drive    function    biological    breadth    rearrange    virus    machine    obtain    intermediates    restructure    compartments    machinery    derive    arranged    complemented    biophysics    complexes    context    electron    carefully    closer    decades    synaptic    model    viral    structural    synapses    mutating    cellular    until    assembled    environments    correlative    microscopy    induce    arrange    draws    components    prior    refolding    material    machineries    led    phenotypes    regulation    membrane    inhibited    viruses    transfer    structurally    dynamic    vitro    found    life    gaps    hiv    vivo    influenza    organism    entry    reshape    regulated    despite    structure    fuses    site    data    combination    fusion    situ    dynamics    little    image    biology    cells    reposition    vesicles    shortage    individual    heterogeneous    tomography    ing    inhibiting    attributed   

Project "MEMBRANEFUSION" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

There are not information about this coordinator. Please contact Fabio for more information, thanks.

 Coordinator Country United Kingdom [UK]
 Total cost 1˙965˙961 €
 EC max contribution 1˙965˙961 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 1˙785˙961.00
2    MEDICAL RESEARCH COUNCIL UK (SWINDON) coordinator 0.00
3    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) participant 180˙000.00

Map

 Project objective

Fusion of two biological membranes is essential to life. It is required during organism development, for trafficking of material between cellular compartments, for transfer of information across synapses, and for entry of viruses into cells. Fusion must be carefully controlled and the core fusion components are typically found within a complex regulatory machine. There have been decades of research on the structure and function of individual components, on the dynamics and biophysics of fusion, and on phenotypes resulting from mutating or inhibiting component proteins. These have led to a model for fusion in which regulated refolding or assembly of proteins draws two membranes closer together until they fuse. Despite this breadth of study, we know very little about how the components of the fusion machinery function in context: How are they arranged on the membrane around the site of fusion? How do they respond structurally to regulation? How does the fully assembled machinery rearrange to reshape the membrane and drive fusion? These gaps in knowledge can be attributed to a shortage of structural biology methods able to derive structural data on proteins assembled within complex, heterogeneous or dynamic environments such as a fusion site. Here I propose to apply a combination of state-of-the-art cryo-electron tomography, image processing and correlative fluorescence and electron microscopy methods to obtain detailed structural information on assembled fusion machineries and of fusion intermediates both in vitro and in vivo. I will study how influenza virus fuses with a target membrane, complemented by studies on fusion of HIV-1 and of synaptic vesicles. By determining how viral and synaptic fusion complexes reposition and restructure prior to fusion, how they arrange around the fusion site, how they reshape the membrane to induce fusion, and how these processes can be regulated and inhibited, I will derive a mechanistic model of membrane fusion in situ.

 Publications

year authors and title journal last update
List of publications.
2020 Robert A. Dick, Chaoyi Xu, Dustin R. Morado, Vladyslav Kravchuk, Clifton L. Ricana, Terri D. Lyddon, Arianna M. Broad, J. Ryan Feathers, Marc C. Johnson, Volker M. Vogt, Juan R. Perilla, John A. G. Briggs, Florian K. M. Schur
Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly
published pages: e1008277, ISSN: 1553-7374, DOI: 10.1371/journal.ppat.1008277
PLOS Pathogens 16/1 2020-03-05
2019 Lauren Ann Metskas, John A. G. Briggs
Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy
published pages: 1-8, ISSN: 1431-9276, DOI: 10.1017/s1431927619000606
Microscopy and Microanalysis 2019-06-06
2017 William Wan, Larissa Kolesnikova, Mairi Clarke, Alexander Koehler, Takeshi Noda, Stephan Becker & John A. G. Briggs
Structure and assembly of the Ebola virus nucleocapsid
published pages: , ISSN: 0028-0836, DOI:
Nature 2019-06-06
2017 Beata Turoňová, Florian K.M. Schur, William Wan, John A.G. Briggs
Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4 Ã…
published pages: 187-195, ISSN: 1047-8477, DOI: 10.1016/j.jsb.2017.07.007
Journal of Structural Biology 199/3 2019-06-06

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The information about "MEMBRANEFUSION" are provided by the European Opendata Portal: CORDIS opendata.

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