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InterAcTEV

Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection

Total Cost €

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EC-Contrib. €

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Partnership

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Project "InterAcTEV" data sheet

The following table provides information about the project.

Coordinator
AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS 

Organization address
address: CALLE SERRANO 117
city: MADRID
postcode: 28006
website: http://www.csic.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Project website https://www.researchgate.net/project/Genome-wide-analysis-of-RNA-and-protein-interacting-profiles-during-a-plant-virus-infection-InterAcTEV-H2020-Marie-S-Curie-Reintegration-Grant-655841
 Total cost 170˙121 €
 EC max contribution 170˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2017-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS ES (MADRID) coordinator 170˙121.00

Map

 Project objective

Plant ()strand RNA viruses account for important losses in crop yields worldwide. They complete complex infectious cycles by replicating, moving and inactivating plant defense mechanisms through multiple interactions of their RNA or proteins with host factors. Plants have evolved two main antiviral immunity systems operated by ARGONAUTE (AGO) proteins that target viral RNAs in the RNA silencing pathway or by resistance proteins that recognize viral-encoded proteins through protein-protein interactions. However, neither the identification of AGO target sites in viral RNAs nor the elucidation of the entire repertoire of proteins interacting with viral proteins has been reported for any plant virus. Now, the recent development of new technologies (and associated tools) such as high-throughput DNA sequencing and high performance mass spectrometry (MS) platforms makes possible to address these questions at a genome-wide level. InterAcTEV will combine these new technologies to study the recently described Arabidopsis/Tobacco etch virus (TEV)-TAMPS pathosystem with a multidisciplinary approach aimed to identify AGO target sites in TEV RNAs and to generate the first complete protein-protein interactome map for a plant virus. First, RNA-immunoprecipitation coupled with high-throughput sequencing methods will be optimized to identify AGO target sites in TEV RNAs at a genome-wide level. Second, we will combine novel high-affinity purification methods with MS analyses to identify the specific pool of Arabidopsis proteins interacting with each TEV protein. InterAcTEV will generate key knowledge to open new avenues for the understanding of resistance mechanisms in plants and to develop novel antiviral strategies for crop protection and securement of world’s food supply. Finally, the funding of InterAcTEV will allow the Experienced Researcher to initiate his own research project in the host laboratory and facilitate his transition to an independent research position in Europe.

 Publications

year authors and title journal last update
List of publications.
2017 Alberto Carbonell, José-Antonio Daròs
Artificial microRNAs and synthetic trans -acting small interfering RNAs interfere with viroid infection
published pages: , ISSN: 1464-6722, DOI: 10.1111/mpp.12529
Molecular Plant Pathology 2019-07-23
2016 Alberto Carbonell, James C. Carrington, and Jose-Antonio Daròs
Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants
published pages: e1130, ISSN: 2375-2467, DOI: 10.14800/rd.1130
RNA & DISEASE 3 2019-07-23
2017 Erzsébet Kenesi, Alberto Carbonell, Rita Lózsa, Beáta Vértessy, Lóránt Lakatos
A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC
published pages: , ISSN: 0305-1048, DOI: 10.1093/nar/gkx379
Nucleic Acids Research 2019-07-23
2017 Teresa Cordero, Lidia Cerdán, Alberto Carbonell, Konstantina Katsarou, Kriton Kalantidis, José-Antonio Daròs
Dicer-Like 4 Is Involved in Restricting the Systemic Movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
published pages: 63-71, ISSN: 0894-0282, DOI: 10.1094/MPMI-11-16-0239-R
Molecular Plant-Microbe Interactions 30/1 2019-07-23
2015 Alberto Carbonell, James C Carrington
Antiviral roles of plant ARGONAUTES
published pages: 111-117, ISSN: 1369-5266, DOI: 10.1016/j.pbi.2015.06.013
Current Opinion in Plant Biology 27 2019-07-23

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