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Opendata, web and dolomites

NextGen RiBiomics

Next Generation Proteomic Analysis of Pre-Ribosomal Proteome Dynamics Coupled to Glucose Metabolism in Caner Cells

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

NextGen RiBiomics project word cloud

Explore the words cloud of the NextGen RiBiomics project. It provides you a very rough idea of what is the project "NextGen RiBiomics" about.

integration silac quantitative ribi suppressed ngp host laboratory deprivation technological 47s analyze human eukaryotic nucleolar isolation first corresponding systematic components community proteome mcf7 generation ribosomal respectively cell 90s thanks compare intracellular biogenesis status glucose deregulation strategies grown me expertise cellular treatment methodology ribosome measuring connection dynamics energy solid combining training 40s incorporate lines cells aid 60s varied instrumentation ribosomes characterised lamond proteins software hela rrna refers regulate purification frequently showing particles occurs space isolated adenocarcinoma tumour consuming proteomics contained database data peptracker cancer group searchable yeast carry time proceeds survival optimise

Project "NextGen RiBiomics" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITY OF DUNDEE Organization address address: Nethergate city: DUNDEE postcode: DD1 4HN website: www.dundee.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Project website	http://www.lamondlab.com/
Total cost	195˙454 €
EC max contribution	195˙454 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2016
Duration (year-month-day)	from 2016-04-01 to 2018-03-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	UNIVERSITY OF DUNDEE UNIVERSITY OF DUNDEE Organization address address: Nethergate city: DUNDEE postcode: DD1 4HN website: www.dundee.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (DUNDEE)	coordinator	195˙454.00

Map

Project objective

The research field of proteomics proceeds rapidly thanks to recent technological advances of instrumentation, methodology, and software development. Next Generation Proteomics (NGP) refers to the integration of these three areas to provide a systematic approach for measuring proteome dynamics in both time and space during various cellular responses. In this proposal, I will analyze pre-ribosomal proteome dynamics in response to intracellular energy status using a NGP approach. Specifically, I will determine how cancer cells regulate Ribosome Biogenesis (RiBi) to aid their survival under conditions of energy deprivation, which frequently occurs in connection with solid tumour development. The host laboratory is well known for developing and applying NGP strategies and will provide me with training and access to all of the equipment and resources required. To carry out this project I will first optimise methods for purification of human pre-ribosomal particles, combining my existing knowledge of RiBi with expertise from the Lamond group in nucleolar isolation. I will then use a quantitative proteomics approach to analyze pre-ribosomes isolated from cells grown under conditions of varied glucose deprivation. I will compare the components of pre-40S, pre-60S and pre-90S particles, respectively, using SILAC and the PepTracker software developed in the Lamond group. This project is based on my recent data showing that 47S pre-rRNA processing, which occurs in pre-90S particles, is suppressed by glucose deprivation in human adenocarcinoma HeLa and MCF7 cell lines. As the proteins contained in human pre-ribosomes are less well characterised than the corresponding yeast proteins, I will incorporate these results in a searchable database of human RiBi factors that will be freely available to the community. As RiBi is the most energy-consuming process in eukaryotic cell the results of this project may lead to novel cancer treatment strategies which target deregulation of RiBi.

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The information about "NEXTGEN RIBIOMICS" are provided by the European Opendata Portal: CORDIS opendata.