Opendata, web and dolomites

NextGen RiBiomics

Next Generation Proteomic Analysis of Pre-Ribosomal Proteome Dynamics Coupled to Glucose Metabolism in Caner Cells

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NextGen RiBiomics project word cloud

Explore the words cloud of the NextGen RiBiomics project. It provides you a very rough idea of what is the project "NextGen RiBiomics" about.

measuring    analyze    tumour    yeast    proceeds    methodology    characterised    intracellular    showing    proteome    incorporate    aid    software    varied    ribosome    isolation    47s    training    cell    database    frequently    ngp    40s    group    isolated    ribosomal    optimise    occurs    strategies    space    deregulation    refers    adenocarcinoma    90s    status    consuming    rrna    ribosomes    cellular    nucleolar    thanks    lines    corresponding    data    first    compare    ribi    expertise    hela    systematic    me    solid    host    human    peptracker    silac    community    combining    instrumentation    glucose    integration    connection    purification    regulate    generation    survival    technological    respectively    carry    60s    searchable    quantitative    particles    proteomics    mcf7    deprivation    dynamics    components    lamond    time    cells    eukaryotic    laboratory    cancer    treatment    contained    proteins    biogenesis    suppressed    energy    grown   

Project "NextGen RiBiomics" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITY OF DUNDEE 

Organization address
address: Nethergate
city: DUNDEE
postcode: DD1 4HN
website: www.dundee.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.lamondlab.com/
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITY OF DUNDEE UK (DUNDEE) coordinator 195˙454.00

Map

 Project objective

The research field of proteomics proceeds rapidly thanks to recent technological advances of instrumentation, methodology, and software development. Next Generation Proteomics (NGP) refers to the integration of these three areas to provide a systematic approach for measuring proteome dynamics in both time and space during various cellular responses. In this proposal, I will analyze pre-ribosomal proteome dynamics in response to intracellular energy status using a NGP approach. Specifically, I will determine how cancer cells regulate Ribosome Biogenesis (RiBi) to aid their survival under conditions of energy deprivation, which frequently occurs in connection with solid tumour development. The host laboratory is well known for developing and applying NGP strategies and will provide me with training and access to all of the equipment and resources required. To carry out this project I will first optimise methods for purification of human pre-ribosomal particles, combining my existing knowledge of RiBi with expertise from the Lamond group in nucleolar isolation. I will then use a quantitative proteomics approach to analyze pre-ribosomes isolated from cells grown under conditions of varied glucose deprivation. I will compare the components of pre-40S, pre-60S and pre-90S particles, respectively, using SILAC and the PepTracker software developed in the Lamond group. This project is based on my recent data showing that 47S pre-rRNA processing, which occurs in pre-90S particles, is suppressed by glucose deprivation in human adenocarcinoma HeLa and MCF7 cell lines. As the proteins contained in human pre-ribosomes are less well characterised than the corresponding yeast proteins, I will incorporate these results in a searchable database of human RiBi factors that will be freely available to the community. As RiBi is the most energy-consuming process in eukaryotic cell the results of this project may lead to novel cancer treatment strategies which target deregulation of RiBi.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NEXTGEN RIBIOMICS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NEXTGEN RIBIOMICS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MultiSeaSpace (2019)

Developing a unified spatial modelling strategy that accounts for interactions between species at different marine trophic levels, and different types of survey data.

Read More  

Topo-circuit (2019)

Exploring topological phenomenon in RF circuits

Read More  

AMPLE (2019)

A Study of the Notion of Ampleness in Model Theory and Tits Buildings

Read More