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Dissecting the epigenetic control of totipotency.

Total Cost €


EC-Contrib. €






 TOTIPOTENCY2014 project word cloud

Explore the words cloud of the TOTIPOTENCY2014 project. It provides you a very rough idea of what is the project "TOTIPOTENCY2014" about.

interdependencies    differentiation    host    lack    enhanced    genes    data    single    implantation    epigenetics    researcher    uses    validating    technologies    rare    34    expertise    therapy    tractable    experimentally    one    mechanistic    screened    confirmed    innovative    developmental    capability    medicine    proteins    regulation    restoring    extra    mode    somatic    potentially    escs    contribution    interaction    action    fertilization    personalised    systematically    population    cells    bioinformatic    stem    eel    cell    additional    model    vitro    embryonic    previously    relevance    regenerative    mechanisms    edge    shortlisted    enhancer    epigenetic    efficiency    expands    biology    chromatin    firstly    transcriptome    enhancers    murine    cutting    combines    determined    poorly    mechanistically    validated    reprogramming    embryo    analysed    am    mammalian    fidelity    regulators    limited    gene    occurs    restricted    lab    expression    normally    expressing    sub    insights    totipotency    critical    shortly    stage    remodelling    newly    contain   

Project "TOTIPOTENCY2014" data sheet

The following table provides information about the project.


Organization address
address: Babraham Hall
postcode: CB22 3AT

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2017-12-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE BABRAHAM INSTITUTE UK (CAMBRIDGE) coordinator 183˙454.00


 Project objective

One of the most critical epigenetic and chromatin remodelling processes in mammalian development occurs shortly after fertilization restoring totipotency. Due to limited cell numbers and lack of experimentally tractable systems, the mechanisms and regulation of this developmental stage are poorly understood. This proposal will provide important mechanistic insights into the epigenetic control of early embryonic gene expression, and its relevance to somatic reprogramming.

Murine embryonic stem cells (ESCs) contain a rare sub-population of early embryonic like (EEL) cells expressing genes normally restricted to the pre-implantation embryo, with enhanced extra-embryonic differentiation capability. Firstly, using novel single-cell technologies, I will characterize the EEL cells in detail, validating them as an in vitro model of pre-implantation development. On-going research in the host lab that I am involved in has identified an epigenetic enhancer that expands this EEL sub-population. Through bioinformatic analysis of pre-implantation transcriptome data, I have shortlisted 34 epigenetic and chromatin-associated proteins, including the previously identified factor, which will be systematically screened to identify new enhancers of totipotency. Validated EEL regulators, including two additional newly confirmed factors, will be analysed mechanistically determining their interaction partners, interdependencies, and mode of action. Finally, the contribution of the totipotency regulators towards the efficiency and fidelity of somatic reprogramming will be determined, potentially improving the use of this technology for personalised gene therapy and regenerative medicine.

This state-of-the-art proposal uses innovative and novel cutting-edge technologies and combines the expertise of the researcher and the host. It has the potential for significant impact across several fields from epigenetics and stem cell biology to reprogramming and regenerative medicine.


year authors and title journal last update
List of publications.
2016 Mélanie A. Eckersley-Maslin, Valentine Svensson, Christel Krueger, Thomas M. Stubbs, Pascal Giehr, Felix Krueger, Ricardo J. Miragaia, Charalampos Kyriakopoulos, Rebecca V. Berrens, Inês Milagre, Jörn Walter, Sarah A. Teichmann, Wolf Reik
MERVL/Zscan4 Network Activation Results in Transient Genome-wide DNA Demethylation of mESCs
published pages: 179-192, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.08.087
Cell Reports 17/1 2019-06-14

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The information about "TOTIPOTENCY2014" are provided by the European Opendata Portal: CORDIS opendata.

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