Opendata, web and dolomites

smSTRUCT TERMINATED

Fuse smFRET and modeling to a new structural biology method and solve the functional ESCRT assembly structure

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 smSTRUCT project word cloud

Explore the words cloud of the smSTRUCT project. It provides you a very rough idea of what is the project "smSTRUCT" about.

cells    contacts    carlo    fuse    model    structural    fact    imaged    invaginated    assembly    infected    infections    blocks    released    single    lipid    efficiencies    cy5    bilayers    machinery    flexibility    biology    methodology    determined    membranes    reveal    individual    stepwise    microscopy    formations    efficiency    biochemistry    sites    escrts    intend    cy3    overdetermined    barriers    reconstructed    structures    throughput    proteins    computational    cell    distance    conformation    gt    contact    defeat    escrt    assembled    experiments    stoichiometry    seek    building    local    modeling    experimental    fret    block    label    composition    labeled    combine    seizes    largely    data    assemblies    monte    alterations    full    ing    yeast    copy    derive    photobleaching    molecule    smfret    functional    computationally    simulations    size    tirf    generate    recorded    labeling    protein    atomistic    structure    distances    complexes    overcome    exchange    unknown    hiv    replica    solving   

Project "smSTRUCT" data sheet

The following table provides information about the project.

Coordinator
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV 

Organization address
address: HOFGARTENSTRASSE 8
city: Munich
postcode: 80539
website: www.mpg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website http://www.schoeneberglab.org
 Total cost 257˙860 €
 EC max contribution 257˙860 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-GF
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2018-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (Munich) coordinator 257˙860.00
2    THE REGENTS OF THE UNIVERSITY OF CALIFORNIA US (OAKLAND CA) partner 0.00

Map

 Project objective

We seek to develop a new structural biology method that is able to overcome barriers to solving very complex functional protein assemblies that are variable enough in their composition and conformation to defeat current methodologies. I intend to combine high-throughput single molecule FRET (smFRET) experiments with computational modeling to achieve this goal. SmFRET will be used to derive individual building block structures as well as distances between these blocks on a molecule-to-molecule level. Computational modeling is used to fuse this information into a full atomistic model of the protein assembly. The yeast ESCRT machinery is proposed as a model system to develop the new methodology. The ESCRT machinery is particularly important because of its role in HIV infections: HIV seizes control of the cell’s ESCRTs to get released from infected cells. The ESCRT assemblies’ size and flexibility lead to the fact that their assembled structure on membranes is largely unknown. Individual ESCRT proteins will be labeled by Cy3/Cy5. The ESCRT assembly will then be reconstructed on invaginated supported lipid bilayers and imaged via TIRF microscopy. FRET efficiencies will be recorded and the label-label distance determined. High-throughput biochemistry and labeling technology will allow us to generate > 100 distinct labeling sites, resulting in overdetermined structures. Stepwise photobleaching will reveal the stoichiometry within full assemblies. Alterations in FRET efficiency due to local contact formations within the assembly will reveal these local contacts. Based on the experimental data of the individual complexes, their copy number in the assembly and their local contacts, the full assembly will be determined computationally, based on replica exchange Monte Carlo simulations.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SMSTRUCT" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "SMSTRUCT" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

CLIMACY (2020)

Practices of Climate Diplomacy and Uneven Policy Responses on Climate Change on Human Mobility

Read More  

Photonic Radar (2019)

Implementation of Long Reach Hybrid Photonic Radar System and convergence over FSO and PON Networks

Read More  

DIGILEAD (2020)

Digital leadership, well-being and performance in organizations

Read More