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smSTRUCT TERMINATED

Fuse smFRET and modeling to a new structural biology method and solve the functional ESCRT assembly structure

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 smSTRUCT project word cloud

Explore the words cloud of the smSTRUCT project. It provides you a very rough idea of what is the project "smSTRUCT" about.

complexes    contact    blocks    labeling    methodology    released    assemblies    recorded    cy5    formations    fret    exchange    largely    conformation    assembly    infected    cells    replica    biology    model    structure    gt    overcome    alterations    cy3    monte    assembled    escrt    label    defeat    local    bilayers    derive    size    escrts    building    photobleaching    computational    data    protein    combine    structures    reconstructed    carlo    computationally    tirf    determined    efficiency    machinery    modeling    distance    seek    intend    solving    imaged    composition    smfret    labeled    individual    stoichiometry    biochemistry    barriers    hiv    distances    invaginated    infections    single    molecule    structural    throughput    generate    fuse    fact    atomistic    stepwise    overdetermined    microscopy    flexibility    efficiencies    seizes    block    experimental    sites    experiments    contacts    membranes    lipid    simulations    proteins    copy    cell    yeast    ing    full    functional    unknown    reveal   

Project "smSTRUCT" data sheet

The following table provides information about the project.

Coordinator
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV 

Organization address
address: HOFGARTENSTRASSE 8
city: Munich
postcode: 80539
website: www.mpg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website http://www.schoeneberglab.org
 Total cost 257˙860 €
 EC max contribution 257˙860 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-GF
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2018-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (Munich) coordinator 257˙860.00
2    THE REGENTS OF THE UNIVERSITY OF CALIFORNIA US (OAKLAND CA) partner 0.00

Map

 Project objective

We seek to develop a new structural biology method that is able to overcome barriers to solving very complex functional protein assemblies that are variable enough in their composition and conformation to defeat current methodologies. I intend to combine high-throughput single molecule FRET (smFRET) experiments with computational modeling to achieve this goal. SmFRET will be used to derive individual building block structures as well as distances between these blocks on a molecule-to-molecule level. Computational modeling is used to fuse this information into a full atomistic model of the protein assembly. The yeast ESCRT machinery is proposed as a model system to develop the new methodology. The ESCRT machinery is particularly important because of its role in HIV infections: HIV seizes control of the cell’s ESCRTs to get released from infected cells. The ESCRT assemblies’ size and flexibility lead to the fact that their assembled structure on membranes is largely unknown. Individual ESCRT proteins will be labeled by Cy3/Cy5. The ESCRT assembly will then be reconstructed on invaginated supported lipid bilayers and imaged via TIRF microscopy. FRET efficiencies will be recorded and the label-label distance determined. High-throughput biochemistry and labeling technology will allow us to generate > 100 distinct labeling sites, resulting in overdetermined structures. Stepwise photobleaching will reveal the stoichiometry within full assemblies. Alterations in FRET efficiency due to local contact formations within the assembly will reveal these local contacts. Based on the experimental data of the individual complexes, their copy number in the assembly and their local contacts, the full assembly will be determined computationally, based on replica exchange Monte Carlo simulations.

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The information about "SMSTRUCT" are provided by the European Opendata Portal: CORDIS opendata.

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