Opendata, web and dolomites

T6SS-PSEUDO-LIP SIGNED

Type VI-dependent Pseudomonas aeruginosa phospholipases and host manipulation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 T6SS-PSEUDO-LIP project word cloud

Explore the words cloud of the T6SS-PSEUDO-LIP project. It provides you a very rough idea of what is the project "T6SS-PSEUDO-LIP" about.

activation    strategy    isolated    innovative    isolates    elucidate    secretes    phosphorylation    encodes    environment    competitors    t6sss    downstream    phospholipases    fundamental    introduction    clinical    expression    cells    performing    pldb    trans    diseases    tract    instrumental    join    phagocytic    delivering    bacteria    aeruginosa    secreted    confirm    pa    events    action    vitro    entry    contribution    interaction    t6ss    discovered    dependent    bacterial    h1    performed    laboratory    host    clusters    toxins    mediated    basic    link    secretion    screened    upstream    decipher    encoded    nosocomial    murine    model    vi    respiratory    invasion    cell    dissect    newly    infections    pathogen    prevalence    h3    infectious    pld    respectively    overview    dissected    signalling    validate    mutants    virulence    vivo    internalization    pseudomonas    h2    killing    originality    plda    effectors    inhibitors    akt    infection    relevance    assays    pi3k    strains    mechanisms    kingdom   

Project "T6SS-PSEUDO-LIP" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.imperial.ac.uk/mrc-centre-for-molecular-bacteriology-and-infection/research/research-groups/professor-alain-filloux/
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-02-01   to  2018-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 195˙454.00

Map

 Project objective

Introduction

Pseudomonas aeruginosa (Pa), a nosocomial pathogen, secretes a wide range of virulence factors. A recently described secretion pathway is the type VI secretion system (T6SS). Pa encodes three T6SSs, H1-, H2- and H3-T6SS. The best-characterized H1-T6SS is involved in delivering toxins into bacterial competitors.

State-of-the art

Two phospholipases (Pld), PldA and PldB secreted via H2- and H3-T6SS, respectively, were recently identified as trans-kingdom virulence effectors, triggering both killing of bacterial competitors and internalization into non-phagocytic cells. They are encoded within two distinct pld clusters that are not present in all Pa isolates.

Objectives

Our study should (i) decipher the prevalence of the two pld clusters among Pa isolates, (ii) elucidate the role of those Pld during in vivo infection, (iii) confirm the Pld contribution in the T6SS mediated entry, (iv) dissect the PI3K/Akt signalling pathway activation mediated by Pld during Pa entry.

Overview of the action

The aim is to link the fundamental study of new virulence factors with their relevance into clinical isolates.

Methods

Pa strains isolated from infection or environment will be screened for the expression of pld clusters. The role of PldA and PldB as virulence factor will be assessed using a murine model of respiratory tract infection. In vitro invasion assays will be performed to validate the role of PldA in the H2-T6SS-dependent entry in non-phagocytic cells. Upstream and downstream signalling events of Akt phosphorylation induced by PldA and PldB upon infection will be dissected by performing cell infections with mutants and specific inhibitors.

Originality and innovative aspects of the project

Bacteria-host interaction mechanisms are instrumental in the development of infectious diseases. Basic (host laboratory) and clinical (the applicant) research will join to characterize a newly discovered virulence strategy of Pa.

 Publications

year authors and title journal last update
List of publications.
2018 Laurent Dortet, Charlotte Lombardi, François Cretin, Andréa Dessen, Alain Filloux
Pore-forming activity of the Pseudomonas aeruginosa type III secretion system translocon alters the host epigenome
published pages: 378-386, ISSN: 2058-5276, DOI: 10.1038/s41564-018-0109-7
Nature Microbiology 3/3 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "T6SS-PSEUDO-LIP" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "T6SS-PSEUDO-LIP" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

TARGET SLEEP (2020)

Boosting motor learning through sleep and targeted memory reactivation in ageing and Parkinson’s disease

Read More  

DECEYEDE (2020)

The effects of aging in the control of eye movements and its relation to perceptual and motor decisions

Read More