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SHUFFLE SIGNED

Recombination in large genome crop plants

Total Cost €

0

EC-Contrib. €

0

Partnership

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 SHUFFLE project word cloud

Explore the words cloud of the SHUFFLE project. It provides you a very rough idea of what is the project "SHUFFLE" about.

double    hypothesis    location    undergoing    captured    improvement    constraints    rate    ends    individuals    exploited    genetically    approximately    meiosis    packages    mark    drives    phenotypically    putative    meiotic    generation    locations    molecularly    proteins    roles    events    chromosomes    wp1    mutations    crossing    transcriptomics    recombination    induce    physical    strategies    genetic    alleles    during    complementation    crops    pull    regulated    regions    break    resolved    complexes    either    wps    isolate    data    questions    barley    exome    characterise    plant    diversity    combinations    population    strand    sequencing    releases    proteomics    plateaued    players    wp2    relate    redistribute    mutants    critically    thirds    economically    populations    genome    materials    cos    assays    cells    excluding    breeding    understand    co    poor    exchange    cereals    dsb    sites    dna    telomeric    cytologically    modifying    restricted    recombinants   

Project "SHUFFLE" data sheet

The following table provides information about the project.

Coordinator
THE JAMES HUTTON INSTITUTE 

Organization address
address: ERROL ROAD INVERGOWRIE
city: DUNDEE
postcode: DD2 5DA
website: www.hutton.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.barleyhub.org/projects/shuffle/
 Total cost 2˙499˙980 €
 EC max contribution 2˙499˙980 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-ADG
 Funding Scheme ERC-ADG
 Starting year 2015
 Duration (year-month-day) from 2015-10-01   to  2020-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE JAMES HUTTON INSTITUTE UK (DUNDEE) coordinator 2˙499˙980.00

Map

 Project objective

During meiosis, recombination (crossing-over, CO) drives the exchange of genetic materials and releases genetic diversity by creating new combinations of alleles within and among chromosomes. CO is exploited in plant breeding through the generation of large populations of recombinants from which genetically improved individuals are selected. However in some economically important crops such as barley, the rate of improvement has plateaued. One hypothesis is that this is due to constraints on the locations of CO. In large genome cereals CO is restricted to the ends of chromosomes, excluding approximately two thirds of the genome from the breeding process.

This project focuses on three critically important questions about CO in barley: Why is it restricted to the telomeric ends of chromosomes? What proteins are the key players and what are their roles in controlling CO? And, what strategies can be established to effectively increase or redistribute CO in CO-poor regions?

I will address these questions in four Work Packages. In WP1 I will induce, identify and molecularly characterise putative meiotic mutants both phenotypically (at the plant level) and molecularly by captured exome sequencing. In WP2 I will characterise these mutants cytologically, genetically and by complementation to understand how the mutations affect recombination. In WPs 3 and 4, I will apply novel methods to isolate the specific cells undergoing meiosis, and use transcriptomics, targeted proteomics and pull-down assays to investigate how meiosis is regulated. I will focus on complexes that mark the sites of DNA double strand break (DSB) formation that identify where CO will occur. I will characterise these sites according to those resolved as either crossing-over or non-crossing-over events and relate their physical location to COs observed in population genetic data. Finally, I will evaluate strategies for modifying the distribution of recombination, and application in plant breeding

 Publications

year authors and title journal last update
List of publications.
2016 Abdellah Barakate, Jennifer Stephens
An Overview of CRISPR-Based Tools and Their Improvements: New Opportunities in Understanding Plant–Pathogen Interactions for Better Crop Protection
published pages: 765, ISSN: 1664-462X, DOI: 10.3389/fpls.2016.00765
Frontiers in Plant Science 7 2019-07-04
2016 Isabelle Colas, Malcolm Macaulay, James D. Higgins, Dylan Phillips, Abdellah Barakate, Markus Posch, Susan J. Armstrong, F. Chris H. Franklin, Claire Halpin, Robbie Waugh, Luke Ramsay
A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10
published pages: 693-707, ISSN: 0028-646X, DOI: 10.1111/nph.14061
New Phytologist 212/3 2019-07-04
2016 Isabelle Colas, Katie Baker, Andrew Flavell
Cytology and Microscopy: Immunolocalization of Covalently Modified Histone Marks on Barley Mitotic Chromosomes
published pages: , ISSN: 2331-8325, DOI: 10.21769/BioProtoc.1841
BIO-PROTOCOL 6/12 2019-07-04
2017 Isabelle Colas, Benoit Darrier, Mikel Arrieta, Sybille U. Mittmann, Luke Ramsay, Pierre Sourdille, Robbie Waugh
Observation of Extensive Chromosome Axis Remodeling during the “Diffuse-Phase” of Meiosis in Large Genome Cereals
published pages: , ISSN: 1664-462X, DOI: 10.3389/fpls.2017.01235
Frontiers in Plant Science 8 2019-05-14
2018 Colas I, Macaulay M, Higgins JD, Phillips D, Barakate A, Posch M, Armstrong SJ, Franklin FC, Halpin C, Waugh R, Ramsay L
Corrigendum
published pages: 348-348, ISSN: 0028-646X, DOI: 10.1111/nph.15402
New Phytologist 220/1 2019-05-14

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