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ACTOMYOSIN RING SIGNED

Understanding Cytokinetic Actomyosin Ring Assembly Through Genetic Code Expansion, Click Chemistry, DNA origami, and in vitro Reconstitution

Total Cost €

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EC-Contrib. €

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 ACTOMYOSIN RING project word cloud

Explore the words cloud of the ACTOMYOSIN RING project. It provides you a very rough idea of what is the project "ACTOMYOSIN RING" about.

microscopy    expansion    dimeric    biological    organization    cellular    principles    origami    spheroplasts    organize    live    employing    division    themselves    genetic    cells    hampered    mechanism    native    bisect    debated    cytoskeletal    generate    cell    illuminate    structural    code    modulators    functional    fission    contractile    appropriate    actively    reconstitute    conserved    click    lack    eukaryotes    arrangement    ring    vi    actin    permeabilized    myosin    internal    pombe    filaments    schizosaccharomyces    visualize    expressing    genetics    filamentous    labelled    comprising    assembled    cytokinesis    chemistry    synthetic    dna    generates    actomyosin    bilayers    yeast    pertaining    questions    daughters    150    filament    efforts    biochemistry    self    assembly    reflection    fluorescence    fundamental    imaging    polarity    mother    force    total    fluorescent    multimeric    decipher    proteins    divides    attempt    myosins    man    biology    machines    motors   

Project "ACTOMYOSIN RING" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF WARWICK 

Organization address
address: Kirby Corner Road - University House
city: COVENTRY
postcode: CV4 8UW
website: www.warwick.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://mechanochemistry.org/Balasubramanian/
 Total cost 2˙863˙705 €
 EC max contribution 2˙863˙705 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-ADG
 Funding Scheme ERC-ADG
 Starting year 2015
 Duration (year-month-day) from 2015-11-01   to  2020-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF WARWICK UK (COVENTRY) coordinator 2˙863˙705.00

Map

 Project objective

The mechanism of cell division is conserved in many eukaryotes, from yeast to man. A contractile ring of filamentous actin and myosin II motors generates the force to bisect a mother cell into two daughters. The actomyosin ring is among the most complex cellular machines, comprising over 150 proteins. Understanding how these proteins organize themselves into a functional ring with appropriate contractile properties remains one of the great challenges in cell biology. Efforts to generate a comprehensive understanding of the mechanism of actomyosin ring assembly have been hampered by the lack of structural information on the arrangement of actin, myosin II, and actin modulators in the ring in its native state. Fundamental questions such as how actin filaments are assembled and organized into a ring remain actively debated. This project will investigate key issues pertaining to cytokinesis in the fission yeast Schizosaccharomyces pombe, which divides employing an actomyosin based contractile ring, using the methods of genetics, biochemistry, cellular imaging, DNA origami, genetic code expansion, and click chemistry. Specifically, we will (1) attempt to visualize actin filament assembly in live cells expressing fluorescent actin generated through synthetic biological approaches, including genetic code expansion and click chemistry (2) decipher actin filament polarity in the actomyosin ring using total internal reflection fluorescence microscopy of labelled dimeric and multimeric myosins V and VI generated through DNA origami approaches (3) address when, where, and how actin filaments for cytokinesis are assembled and organized into a ring and (4) reconstitute actin filament and functional actomyosin ring assembly in permeabilized spheroplasts and in supported bilayers. Success in the project will provide major insight into the mechanism of actomyosin ring assembly and illuminate principles behind cytoskeletal self-organization.

 Publications

year authors and title journal last update
List of publications.
2018 Tzer Chyn Lim, Tomoyuki Hatano, Anton Kamnev, Mohan K. Balasubramanian, Ting Gang Chew
Equatorial Assembly of the Cell-Division Actomyosin Ring in the Absence of Cytokinetic Spatial Cues
published pages: 955-962.e3, ISSN: 0960-9822, DOI: 10.1016/j.cub.2018.01.088
Current Biology 28/6 2019-07-05
2018 Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T. Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-y-Lazo, Alexandra M. E. Jones, Robert C. Robinson, Karuna Sampath, Masanori Mishima, Andrew D. McAinsh, Bruce L. Goode, Mohan K. Balasubramanian
Rapid production of pure recombinant actin isoforms in Pichia pastoris
published pages: jcs213827, ISSN: 0021-9533, DOI: 10.1242/jcs.213827
Journal of Cell Science 131/8 2019-07-05
2018 Saravanan Palani, Ramanujam Srinivasan, Paola Zambon, Anton Kamnev, Pananghat Gayathri, Mohan K. Balasubramanian
Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast
published pages: jcs205625, ISSN: 0021-9533, DOI: 10.1242/jcs.205625
Journal of Cell Science 131/1 2019-07-05

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