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Unraveling interaction networks of ammonia- and nitrite-oxidizing microorganisms

Total Cost €


EC-Contrib. €






 iNet project word cloud

Explore the words cloud of the iNet project. It provides you a very rough idea of what is the project "iNet" about.

oxidizing    initiates    ozone    mutualistic    first    algal    artificially    interaction    attack    metabolic    contributes    isolate    environment    run    prime    cultures    cross    functional    autotrophic    contrast    nitrifying    interact    shaping    metabolism    excess    greenhouse    metabolites    bacteria    waters    networks    eutrophication    donor    global    cycles    nitrogen    uncultured    feed    effect    patterns    true    oxide    groups    oxidized    nitrate    analyze    interplay    allies    biogeochemical    gene    productivity    feeding    biological    expression    occurrence    electron    emission    nitrous    gas    nitrifiers    nitrite    predator    analyzing    techniques    structured    isolates    occurring    heterotrophic    released    nature    microbes    environments    assemblages    simplify    dynamics    prey    ecosystems    separately    stability    surface    nitrification    ing    soils    performance    microbial    expand    isolation    mixed    wastewater    physiological    reduce    nob    individual    shape    load    off    drive    depleting    removal    blooms    treatment    converting    ammonia    interactions    aob    obtain    grown    co    fertilized   

Project "iNet" data sheet

The following table provides information about the project.


Organization address
postcode: 6525 EZ

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 175˙572 €
 EC max contribution 175˙572 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-02-01   to  2022-01-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Microbial interactions drive global biogeochemical cycles and shape functionality and productivity of ecosystems. Autotrophic nitrification is a prime example for a mutualistic interplay of two functional groups. In fertilized soils, nitrification contributes to emission of the ozone-depleting greenhouse gas nitrous oxide and nitrogen (N) run-off into surface waters, resulting in eutrophication and algal blooms. In contrast, in biological wastewater treatment it initiates the removal of excess N to reduce the N load into the environment. In their cross-feeding interaction, ammonia-oxidizing bacteria (AOB) provide the electron donor for their partner by converting ammonia to nitrite, which is further oxidized to nitrate by nitrite-oxidizing bacteria (NOB). In addition, nitrifiers also interact with heterotrophic bacteria that feed on metabolites released by nitrifiers or attack them in a predator-prey interaction. In this project, I aim to determine factors defining interaction networks of nitrifiers by analyzing the effect of this interplay on the metabolism of the individual allies and by identifying novel interaction partners of nitrifying microbes. I will analyze the metabolic aspects of this interplay in artificially mixed nitrifying assemblages to simplify the complex interaction networks occurring in nature. Using state-of-the-art isolation techniques, I will first isolate novel AOB and NOB cultures from the same environment to obtain true interaction partners. Physiological key features and gene expression patterns of separately grown and mixed cultures of these isolates will be compared to determine the nature and effects of interactions among nitrifiers. In addition, novel interaction partners of uncultured nitrifiers will be identified by analyzing co-occurrence patterns within spatially structured environments. Overall, this project will greatly expand our understanding of nitrification and the dynamics shaping its stability and process performance.

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The information about "INET" are provided by the European Opendata Portal: CORDIS opendata.

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