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Unraveling interaction networks of ammonia- and nitrite-oxidizing microorganisms

Total Cost €


EC-Contrib. €






 iNet project word cloud

Explore the words cloud of the iNet project. It provides you a very rough idea of what is the project "iNet" about.

artificially    soils    surface    ammonia    nitrifiers    attack    ing    biogeochemical    gene    microbial    eutrophication    simplify    removal    aob    performance    oxidizing    nob    contributes    predator    obtain    ecosystems    excess    analyzing    load    prime    true    microbes    shaping    effect    groups    nitrite    biological    patterns    donor    oxidized    initiates    depleting    productivity    individual    algal    networks    greenhouse    nitrogen    nature    mixed    fertilized    interplay    environment    released    feeding    electron    techniques    reduce    interactions    wastewater    stability    assemblages    mutualistic    cycles    isolates    co    nitrification    off    isolation    grown    structured    run    functional    nitrifying    first    cross    oxide    interact    dynamics    cultures    metabolism    expression    environments    nitrous    allies    treatment    contrast    ozone    feed    waters    autotrophic    metabolites    occurring    metabolic    separately    expand    isolate    drive    global    nitrate    prey    blooms    analyze    physiological    emission    uncultured    converting    occurrence    heterotrophic    shape    bacteria    interaction    gas   

Project "iNet" data sheet

The following table provides information about the project.


Organization address
postcode: 6525 EZ

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 175˙572 €
 EC max contribution 175˙572 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-02-01   to  2022-01-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Microbial interactions drive global biogeochemical cycles and shape functionality and productivity of ecosystems. Autotrophic nitrification is a prime example for a mutualistic interplay of two functional groups. In fertilized soils, nitrification contributes to emission of the ozone-depleting greenhouse gas nitrous oxide and nitrogen (N) run-off into surface waters, resulting in eutrophication and algal blooms. In contrast, in biological wastewater treatment it initiates the removal of excess N to reduce the N load into the environment. In their cross-feeding interaction, ammonia-oxidizing bacteria (AOB) provide the electron donor for their partner by converting ammonia to nitrite, which is further oxidized to nitrate by nitrite-oxidizing bacteria (NOB). In addition, nitrifiers also interact with heterotrophic bacteria that feed on metabolites released by nitrifiers or attack them in a predator-prey interaction. In this project, I aim to determine factors defining interaction networks of nitrifiers by analyzing the effect of this interplay on the metabolism of the individual allies and by identifying novel interaction partners of nitrifying microbes. I will analyze the metabolic aspects of this interplay in artificially mixed nitrifying assemblages to simplify the complex interaction networks occurring in nature. Using state-of-the-art isolation techniques, I will first isolate novel AOB and NOB cultures from the same environment to obtain true interaction partners. Physiological key features and gene expression patterns of separately grown and mixed cultures of these isolates will be compared to determine the nature and effects of interactions among nitrifiers. In addition, novel interaction partners of uncultured nitrifiers will be identified by analyzing co-occurrence patterns within spatially structured environments. Overall, this project will greatly expand our understanding of nitrification and the dynamics shaping its stability and process performance.

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The information about "INET" are provided by the European Opendata Portal: CORDIS opendata.

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