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iNet

Unraveling interaction networks of ammonia- and nitrite-oxidizing microorganisms

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 iNet project word cloud

Explore the words cloud of the iNet project. It provides you a very rough idea of what is the project "iNet" about.

assemblages    true    biogeochemical    isolate    excess    feed    mixed    autotrophic    nature    environment    reduce    biological    metabolic    aob    co    shaping    released    treatment    expand    occurrence    groups    effect    feeding    interactions    waters    greenhouse    artificially    performance    soils    occurring    networks    ozone    environments    microbes    prime    interplay    isolates    gas    global    nitrification    oxide    obtain    eutrophication    ing    nitrate    stability    contrast    oxidized    expression    simplify    drive    off    structured    shape    algal    oxidizing    bacteria    first    mutualistic    heterotrophic    techniques    separately    contributes    fertilized    dynamics    nob    metabolites    electron    patterns    physiological    nitrogen    uncultured    depleting    ecosystems    gene    ammonia    converting    microbial    analyzing    donor    prey    isolation    removal    predator    emission    surface    nitrifiers    blooms    productivity    cultures    cycles    allies    wastewater    interaction    initiates    grown    metabolism    run    analyze    nitrifying    individual    attack    load    nitrous    functional    cross    nitrite    interact   

Project "iNet" data sheet

The following table provides information about the project.

Coordinator
STICHTING KATHOLIEKE UNIVERSITEIT 

Organization address
address: GEERT GROOTEPLEIN NOORD 9
city: NIJMEGEN
postcode: 6525 EZ
website: www.radboudumc.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 175˙572 €
 EC max contribution 175˙572 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-02-01   to  2022-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    STICHTING KATHOLIEKE UNIVERSITEIT NL (NIJMEGEN) coordinator 175˙572.00

Map

Leaflet | Map data © OpenStreetMap contributors, CC-BY-SA, Imagery © Mapbox

 Project objective

Microbial interactions drive global biogeochemical cycles and shape functionality and productivity of ecosystems. Autotrophic nitrification is a prime example for a mutualistic interplay of two functional groups. In fertilized soils, nitrification contributes to emission of the ozone-depleting greenhouse gas nitrous oxide and nitrogen (N) run-off into surface waters, resulting in eutrophication and algal blooms. In contrast, in biological wastewater treatment it initiates the removal of excess N to reduce the N load into the environment. In their cross-feeding interaction, ammonia-oxidizing bacteria (AOB) provide the electron donor for their partner by converting ammonia to nitrite, which is further oxidized to nitrate by nitrite-oxidizing bacteria (NOB). In addition, nitrifiers also interact with heterotrophic bacteria that feed on metabolites released by nitrifiers or attack them in a predator-prey interaction. In this project, I aim to determine factors defining interaction networks of nitrifiers by analyzing the effect of this interplay on the metabolism of the individual allies and by identifying novel interaction partners of nitrifying microbes. I will analyze the metabolic aspects of this interplay in artificially mixed nitrifying assemblages to simplify the complex interaction networks occurring in nature. Using state-of-the-art isolation techniques, I will first isolate novel AOB and NOB cultures from the same environment to obtain true interaction partners. Physiological key features and gene expression patterns of separately grown and mixed cultures of these isolates will be compared to determine the nature and effects of interactions among nitrifiers. In addition, novel interaction partners of uncultured nitrifiers will be identified by analyzing co-occurrence patterns within spatially structured environments. Overall, this project will greatly expand our understanding of nitrification and the dynamics shaping its stability and process performance.

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The information about "INET" are provided by the European Opendata Portal: CORDIS opendata.

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