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Chemistry and Biology in Synergy - Studies of hydrogenases using a combination of synthetic chemistry and biological tools

Total Cost €


EC-Contrib. €






 CaBiS project word cloud

Explore the words cloud of the CaBiS project. It provides you a very rough idea of what is the project "CaBiS" about.

maturase    enzyme    hydrogenase    interdisciplinary    multitude    hydrogenases    catalytic    oligopeptides    inside    my    model    pt    bio    organometallic    4fe4s    ground    draw    2fe    binding    primary    proteomics    technological    light    vivo    vitro    hydrogen    consequently    elucidate    remarkable    miniaturized    explore    advantage    breaking    interconversion    provides    cofactor    interaction    activate    cell    compounds    arguably    society    spectroscopic    maturation    chemistry    efficiency    proteins    paving    mimics    alternatives    reactivity    h2    parallel    protons    fes    berggren    combination    designing    synthetic    class    tools    appealing    inspired    gain    nature    activation    catalysts    2013    techniques    cluster    prepare    catalyse    function    artificial    forms    metabolomics    metalloproteins    hydf    fefe    ranging    biochemical    al    mature    bioinorganic    thereby    subsite    modification    loaded    intensively    apo    combining    et    applicable    living    preparation    reactions    hyda   

Project "CaBiS" data sheet

The following table provides information about the project.


Organization address
postcode: 751 05

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Total cost 1˙494˙880 €
 EC max contribution 1˙494˙880 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-02-01   to  2022-01-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UPPSALA UNIVERSITET SE (UPPSALA) coordinator 1˙494˙880.00


 Project objective

My proposal aims to take advantage of my ground-breaking finding that it is possible to mature, or activate, the [FeFe] hydrogenase enzyme (HydA) using synthetic mimics of its catalytic [2Fe] cofactor. (Berggren et al, Nature, 2013) We will now explore the chemistry and (bio-)technological potential of the enzyme using an interdisciplinary approach ranging from in vivo biochemical studies all the way to synthetic model chemistry. Hydrogenases catalyse the interconversion between protons and H2 with remarkable efficiency. Consequently, they are intensively studied as alternatives to Pt-catalysts for these reactions, and are arguably of high (bio-) technological importance in the light of a future “hydrogen society”. The project involves the preparation of novel “artificial” hydrogenases with the primary aim of designing spectroscopic model systems via modification(s) of the organometallic [2Fe] subsite. In parallel we will prepare in vitro loaded forms of the maturase HydF and study its interaction with apo-HydA in order to further elucidate the maturation process of HydA. Moreover we will develop the techniques necessary for in vivo application of the artificial activation concept, thereby paving the way for a multitude of studies including the reactivity of artificial hydrogenases inside a living cell, but also e.g. gain-of-function studies in combination with metabolomics and proteomics. Inspired by our work on the artificial maturation system we will also draw from our knowledge of Nature’s [FeS] cluster proteins in order to prepare a novel class of “miniaturized hydrogenases” combining synthetic [4Fe4S] binding oligopeptides with [2Fe] cofactor model compounds. Our interdisciplinary approach is particularly appealing as it not only provides further insight into hydrogenase chemistry and the maturation of metalloproteins, but also involves the development of novel tools and concepts applicable to the wider field of bioinorganic chemistry.


year authors and title journal last update
List of publications.
2020 Lívia Mészáros, Pierre Ceccaldi, Marco Lorenzi, Holly Jayne Redman, Emanuel Pfitzner, Joachim Heberle, Moritz Senger, Sven Timo Stripp, Gustav Berggren
Spectroscopic investigations under whole cell conditions provide new insight into the metal hydride chemistry of [FeFe]-hydrogenase
published pages: , ISSN: 2041-6520, DOI: 10.1039/d0sc00512f
Chemical Science 2020-04-24
2018 Lívia S. Mészáros, Brigitta Németh, Charlène Esmieu, Pierre Ceccaldi, Gustav Berggren
In Vivo EPR Characterization of Semi-Synthetic [FeFe] Hydrogenases
published pages: 2596-2599, ISSN: 1433-7851, DOI: 10.1002/anie.201710740
Angewandte Chemie International Edition 57/10 2019-06-13
2018 C. Esmieu, P. Raleiras, G. Berggren
From protein engineering to artificial enzymes – biological and biomimetic approaches towards sustainable hydrogen production
published pages: 724-750, ISSN: 2398-4902, DOI: 10.1039/C7SE00582B
Sustainable Energy & Fuels 2/4 2019-06-13
2017 N. Khanna, C. Esmieu, L. S. Mészáros, P. Lindblad, G. Berggren
In vivo activation of an [FeFe] hydrogenase using synthetic cofactors
published pages: 1563-1567, ISSN: 1754-5692, DOI: 10.1039/C7EE00135E
Energy & Environmental Science 10/7 2019-06-13
2019 Brigitta Németh, Charlène Esmieu, Holly J. Redman, Gustav Berggren
Monitoring H-cluster assembly using a semi-synthetic HydF protein
published pages: 5978-5986, ISSN: 1477-9226, DOI: 10.1039/c8dt04294b
Dalton Transactions 48/18 2019-08-29
2019 Charlène Esmieu, Meiyuan Guo, Holly J. Redman, Marcus Lundberg, Gustav Berggren
Synthesis of a miniaturized [FeFe] hydrogenase model system
published pages: 2280-2284, ISSN: 1477-9226, DOI: 10.1039/c8dt05085f
Dalton Transactions 48/7 2019-08-29

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