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DropContEvo SIGNED

A droplet microfluidic system for continuous in vivo evolution.

Total Cost €

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EC-Contrib. €

0

Partnership

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 DropContEvo project word cloud

Explore the words cloud of the DropContEvo project. It provides you a very rough idea of what is the project "DropContEvo" about.

specializes    hundreds    skills    confinement    encapsulation    cell    cells    proof    schemes    times    populations    soft    compartments    industrial    oil    enzymes    water    experiments    vitro    broad    unsupervised    vivo    cycle    strategies    evolution    microbiology    screening    passive    dr    droplets    accelerate    acute    content    economically    biologists    single    throughput    biochemistry    sorting    droplet    fresh    microfluidic    larger    reaction    dilution    libraries    directed    of    comprise    nanoliter    ing    containing    biomolecules    evolutionary    tryptophan    small    complementary    efficient    beneficiary    picoliter    hollfelder    cycles    professional    picodroplets    stage    aid    biotechnology    bacteria    laboratory    technologies    specialists    breakthrough    thousands    fluorescence    active    coupled    continuous    splitting    synthase    biotechnologists    series    presenting    extensive    coli    multiple    format    population    absorbance    volume    community    nutrients    trps    training    microfluidics    ultra   

Project "DropContEvo" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2019-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 183˙454.00

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 Project objective

Droplet microfluidics has recently become one of the breakthrough technologies for high throughput screening in microbiology and biochemistry, including single cell studies and new approaches to in vitro evolution. Here we propose a development of a novel microfluidic system for unsupervised execution of multiple cycles of in vivo continuous evolution in hundreds of thousands of picoliter droplets. Each evolutionary cycle will comprise: i) encapsulation of single bacteria cells in water-in-oil compartments ii) growth of the cells coupled with production of economically relevant biomolecules iii) selection of the most efficient populations using ultra-high-throughput sorting of picodroplets, iv) dilution of each population via merging with 100 times larger nanoliter droplet containing fresh nutrients and v) passive splitting of each of the resulting nanoliter droplets to the libraries of picoliter droplets containing single cells. Confinement of the reaction in small volume and active sorting of droplets will facilitate and accelerate the process of in vivo evolution. Droplet format will also enable for various screening schemes, so far not available for continuous evolution strategies – e.g. based on high throughput fluorescence or absorbance measurements of the droplet content. The second stage of the project will comprise a series of proof-of-concept experiments presenting directed continuous evolution of the tryptophan synthase (TrpS) in E.coli bacteria. The technology proposed here would be very useful for broad community of biotechnologists, evolutionary biologists and industrial specialists without the experience in microfluidics. The proposed research will be conducted at Dr. Hollfelder´s laboratory that specializes in directed evolution of enzymes and application of microfluidics to industrial biotechnology. The project comprise broad and extensive training in research and complementary soft skills that will aid professional development of the Beneficiary.

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