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DropContEvo SIGNED

A droplet microfluidic system for continuous in vivo evolution.

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 DropContEvo project word cloud

Explore the words cloud of the DropContEvo project. It provides you a very rough idea of what is the project "DropContEvo" about.

schemes    nutrients    accelerate    broad    soft    compartments    community    specializes    tryptophan    coupled    multiple    picoliter    content    droplet    specialists    proof    cycle    single    technologies    volume    picodroplets    extensive    experiments    directed    containing    biotechnologists    bacteria    oil    efficient    sorting    economically    stage    strategies    nanoliter    vitro    libraries    passive    absorbance    evolutionary    complementary    cell    microfluidic    hollfelder    cycles    format    fluorescence    acute    industrial    cells    aid    dr    vivo    throughput    professional    series    population    presenting    populations    confinement    hundreds    ing    biochemistry    encapsulation    larger    breakthrough    of    microbiology    fresh    reaction    training    dilution    biologists    microfluidics    synthase    screening    continuous    enzymes    active    biotechnology    coli    droplets    small    thousands    water    laboratory    trps    beneficiary    splitting    comprise    unsupervised    biomolecules    evolution    times    ultra    skills   

Project "DropContEvo" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2019-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 183˙454.00

Map

 Project objective

Droplet microfluidics has recently become one of the breakthrough technologies for high throughput screening in microbiology and biochemistry, including single cell studies and new approaches to in vitro evolution. Here we propose a development of a novel microfluidic system for unsupervised execution of multiple cycles of in vivo continuous evolution in hundreds of thousands of picoliter droplets. Each evolutionary cycle will comprise: i) encapsulation of single bacteria cells in water-in-oil compartments ii) growth of the cells coupled with production of economically relevant biomolecules iii) selection of the most efficient populations using ultra-high-throughput sorting of picodroplets, iv) dilution of each population via merging with 100 times larger nanoliter droplet containing fresh nutrients and v) passive splitting of each of the resulting nanoliter droplets to the libraries of picoliter droplets containing single cells. Confinement of the reaction in small volume and active sorting of droplets will facilitate and accelerate the process of in vivo evolution. Droplet format will also enable for various screening schemes, so far not available for continuous evolution strategies – e.g. based on high throughput fluorescence or absorbance measurements of the droplet content. The second stage of the project will comprise a series of proof-of-concept experiments presenting directed continuous evolution of the tryptophan synthase (TrpS) in E.coli bacteria. The technology proposed here would be very useful for broad community of biotechnologists, evolutionary biologists and industrial specialists without the experience in microfluidics. The proposed research will be conducted at Dr. Hollfelder´s laboratory that specializes in directed evolution of enzymes and application of microfluidics to industrial biotechnology. The project comprise broad and extensive training in research and complementary soft skills that will aid professional development of the Beneficiary.

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