Opendata, web and dolomites

ACtIVAtE SIGNED

The virulence potential of human pathogens: how Acinetobacter baumannii survives Acanthamoeba castellanii predation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ACtIVAtE project word cloud

Explore the words cloud of the ACtIVAtE project. It provides you a very rough idea of what is the project "ACtIVAtE" about.

interaction    host    mechanisms    lacking    reporter    context    screen    broadly    cellular    precise    acanthamoeba    fluorescence    health    threat    qualitative    cyst    mutant    cell    throughput    validated    manners    bacterial    tn    complementary    generating    mechanistic    biphasic    live    pathogens    decipher    population    unknown    nosocomial    time    resistance    assays    seq    poorly    pathogen    microscopy    predation    bacteria    individual    symbiotic    model    light    amoebal    deadly    imaging    global    quantitative    monitoring    tested    complemented    levels    superbug    virulence    interactions    strains    single    amoebae    stages    despite    induction    clinical    human    successful    isolates    techniques    viability    acinetobacter    electron    relevance    original    screening    infection    outcome    baumannii    trophozoite    shed    grazing    correlated    castellanii    localization    understand    attenuated    followed    pathogenicity    experimental    amoeba   

Project "ACtIVAtE" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE NAMUR ASBL 

Organization address
address: RUE DE BRUXELLES 61
city: NAMUR
postcode: 5000
website: www.unamur.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 172˙800 €
 EC max contribution 172˙800 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2019-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE NAMUR ASBL BE (NAMUR) coordinator 172˙800.00

Map

 Project objective

The human pathogen Acinetobacter baumannii represents a deadly threat to human health. Despite its established clinical relevance, the pathogenicity of this nosocomial superbug is poorly understood. The biphasic amoeba Acanthamoeba castellanii is a cellular infection model broadly used to decipher the virulence mechanisms of human pathogens, as bacterial resistance upon amoebal predation (grazing) are often correlated with their pathogenicity potential in human. However, high-throughput screening approaches to investigate the virulence mechanisms of A. baumannii using this amoeba in an infection context are still lacking. This project aims at applying original large-scale screening approaches to identify A. baumannii virulence factors using the amoeba A. castellanii as host-pathogen system. The proposed experimental set up allows monitoring the outcome of the bacteria-amoebae interactions in qualitative and quantitative manners, at both population and single cell levels. Virulence induction of A. baumannii, as well as the use of successful clinical isolates will be tested using several complementary grazing assays. Precise localization of A. baumannii reporter strains will be done on both trophozoite and cyst stages of A. castellanii, complemented with (i) real-time and live-cell fluorescence imaging as well as (ii) electron microscopy techniques. Viability assays for both bacteria and amoebae will be done to understand the symbiotic outcome of this interaction. The global resistance potential of A. baumannii will be assessed by (i) Tn-seq analysis and (ii) high-throughput screen of individual mutant strains, followed by in depth mechanistic studies on validated attenuated strains. This project will shed light on the yet unknown global virulence mechanisms of A. baumannii by generating original screening methods using A. castellanii as an infection cellular model.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ACTIVATE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "ACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MSOPGDM (2019)

Mechanistic studies of prokaryotic genome defense mechanisms

Read More  

TeamUp (2020)

Understanding and improving team decision making in uncertain environments

Read More  

EVOMET (2019)

The rise and fall of metastatic clones under immune attack

Read More