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ACtIVAtE SIGNED

The virulence potential of human pathogens: how Acinetobacter baumannii survives Acanthamoeba castellanii predation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 ACtIVAtE project word cloud

Explore the words cloud of the ACtIVAtE project. It provides you a very rough idea of what is the project "ACtIVAtE" about.

population    amoeba    stages    pathogen    infection    model    electron    broadly    threat    virulence    pathogenicity    cell    manners    interaction    predation    shed    despite    baumannii    quantitative    complementary    viability    monitoring    lacking    light    unknown    individual    single    followed    microscopy    complemented    resistance    throughput    original    acanthamoeba    isolates    screen    deadly    bacterial    cellular    biphasic    host    induction    attenuated    tn    validated    levels    qualitative    live    amoebal    cyst    assays    correlated    health    mechanisms    context    global    mechanistic    imaging    bacteria    grazing    clinical    interactions    mutant    understand    strains    time    precise    amoebae    superbug    decipher    trophozoite    pathogens    acinetobacter    generating    castellanii    symbiotic    successful    experimental    human    techniques    relevance    nosocomial    screening    poorly    fluorescence    tested    outcome    localization    seq    reporter   

Project "ACtIVAtE" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE NAMUR ASBL 

Organization address
address: RUE DE BRUXELLES 61
city: NAMUR
postcode: 5000
website: www.unamur.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 172˙800 €
 EC max contribution 172˙800 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2019-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE NAMUR ASBL BE (NAMUR) coordinator 172˙800.00

Map

 Project objective

The human pathogen Acinetobacter baumannii represents a deadly threat to human health. Despite its established clinical relevance, the pathogenicity of this nosocomial superbug is poorly understood. The biphasic amoeba Acanthamoeba castellanii is a cellular infection model broadly used to decipher the virulence mechanisms of human pathogens, as bacterial resistance upon amoebal predation (grazing) are often correlated with their pathogenicity potential in human. However, high-throughput screening approaches to investigate the virulence mechanisms of A. baumannii using this amoeba in an infection context are still lacking. This project aims at applying original large-scale screening approaches to identify A. baumannii virulence factors using the amoeba A. castellanii as host-pathogen system. The proposed experimental set up allows monitoring the outcome of the bacteria-amoebae interactions in qualitative and quantitative manners, at both population and single cell levels. Virulence induction of A. baumannii, as well as the use of successful clinical isolates will be tested using several complementary grazing assays. Precise localization of A. baumannii reporter strains will be done on both trophozoite and cyst stages of A. castellanii, complemented with (i) real-time and live-cell fluorescence imaging as well as (ii) electron microscopy techniques. Viability assays for both bacteria and amoebae will be done to understand the symbiotic outcome of this interaction. The global resistance potential of A. baumannii will be assessed by (i) Tn-seq analysis and (ii) high-throughput screen of individual mutant strains, followed by in depth mechanistic studies on validated attenuated strains. This project will shed light on the yet unknown global virulence mechanisms of A. baumannii by generating original screening methods using A. castellanii as an infection cellular model.

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The information about "ACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.

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