Opendata, web and dolomites

ISA-NPC-ASI TERMINATED

In Situ structural Analysis of Nuclear Pore Complex ASsembly Intermediates by cryo-FIB-tomography

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ISA-NPC-ASI project word cloud

Explore the words cloud of the ISA-NPC-ASI project. It provides you a very rough idea of what is the project "ISA-NPC-ASI" about.

mediating    publicly    appealing    combined    beck    full    cytoplasm    assembled    paths    fundamentally    pore    considerable    assembly    degenerative    holds    anchor    perspective    poorly    structure    embl    goals    mechanism    fundamental    structures    training    npcs    manner    npc    made    subsequently    coordinated    intermediates    1000    cancerogenesis    dr    hence    specimen    structurally    time    mechanistic    compounds    scaffold    electron    macromolecular    examine    strategy    first    elucidated    modified    implicated    structural    supervisor    thinning    host    constitute    cell    analyze    conduct    promise    yeast    division    arrest    molecular    situ    conferences    diseases    genetically    consist    architecture    enrich    complexes    beam    scientific    me    strength    microscopy    basis    exclusive    nucleus    cryo    community    data    audience    independence    therapeutic    supra    context    native    complement    professional    away    expand    ion    nuclear    proteins    tomography    traffic    excellent    infrastructure   

Project "ISA-NPC-ASI" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 159˙460.00

Map

 Project objective

Nuclear pore complexes (NPCs) are fundamental supra-molecular complexes mediating the molecular traffic between nucleus and cytoplasm. They consist of over 1000 proteins that need to be assembled in a coordinated manner each cell division. Recent structural work has elucidated a considerable part of the NPC architecture, however how the NPC scaffold is assembled remains poorly understood on the structural and mechanistic level. Here I propose for the first time to arrest and structurally analyze NPC assembly intermediates. The proposed research aims to enrich the intermediates in genetically modified yeast using an anchor away approach. Subsequently the state-of-the-art Focused-Ion-Beam specimen thinning technology will be combined with cryo Electron-Tomography to examine the intermediates in their native context. The structures of the assembly intermediates will fundamentally advance our understanding of the NPC assembly mechanism that has been strongly implicated in cancerogenesis and various degenerative diseases. The proposed research thus holds great promise to enable the development of novel therapeutic compounds. This project will complement on my strength in using cryo Electron-Microscopy and expand it to in cell studies, hence providing me full professional independence. The supervisor Dr. Beck is very experienced in NPC structure research. EMBL is a well-established host institution and will provide me excellent training and infrastructure to conduct the proposed research. These two factors together constitute a robust basis to reach the goals of the proposal. Produced data will be made publicly available and dissemination of the results in conferences and to a broader audience is an integrated part of the application. In perspective the implementation of this exclusive method will allow investigating other large macromolecular complexes and their assembly paths in situ providing an appealing strategy for the European scientific community.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ISA-NPC-ASI" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "ISA-NPC-ASI" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

NaWaTL (2020)

Narrative, Writing, and the Teotihuacan Language: Exploring Language History Through Phylogenetics, Epigraphy and Iconography

Read More  

MingleIFT (2020)

Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

Read More  

POMOC (2019)

Charles IV and the power of marvellous objects

Read More  
lastchecktime (2022-11-29 15:05:49) correctly updated