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TryptoBoost SIGNED

Boosting tryptophan fluorescence with optical nanoantennas to watch label-free protein dynamics with single molecule resolution at high concentration

Total Cost €

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EC-Contrib. €

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Partnership

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 TryptoBoost project word cloud

Explore the words cloud of the TryptoBoost project. It provides you a very rough idea of what is the project "TryptoBoost" about.

containing    biophysical    folding    team    acid    fluorescent    external    monitor    micromolar    chip    photocatalysis    machineries    signals    probe    nanostructures    enhancement    labeling    applicability    sensitivity    enhanced    life    experiments    nanoantennas    biosensing    overcome    aromatic    hidden    detection    suffer    label    amino    applicable    severely    physiologically    optical    tryptoboost    biological    medical    light    nanomolar    single    suppressor    limit    chemical    therapeutic    analytical    fluorescence    acids    ultimately    interactions    instance    relevance    free    drugs    reveal    amyloid    proteins    natural    drawbacks    broad    tryptophan    techniques    tumor    central    dynamics    molecule    speed    functions    interdisciplinary    thanks    concentrations    breakthroughs    ensemble    p53    aggregation    ultraviolet    rules    physiological    weak    met    averaged    preferred    heterogeneities    read    execute    nanoscale    human    90    intrinsic    power    protein    benefit    localization   

Project "TryptoBoost" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙947˙208 €
 EC max contribution 1˙947˙208 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-10-01   to  2022-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 1˙947˙208.00

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 Project objective

Proteins execute a broad range of functions that are central to life. Understanding these functions ultimately requires experiments at the single protein level to reveal dynamics and heterogeneities hidden in ensemble-averaged measurements. Currently, the preferred method to study single protein machineries is based on fluorescence techniques. However, fluorescence experiments suffer from major challenges: the need for external fluorescent labeling, weak signals and low non-physiological concentrations in the nanomolar range. These challenges severely limit the applicability and biological relevance of single molecule fluorescence on proteins. The TryptoBoost project aims to overcome all the previous challenges, and efficiently monitor single label-free proteins using their intrinsic tryptophan fluorescence enhanced by optical nanoantennas in the ultraviolet. Using the natural amino acid fluorescence rules out all drawbacks due to external labeling, while the optical nanoantennas enable single protein analysis at the physiologically relevant micromolar concentrations thanks to the localization and enhancement of light-matter interactions at the nanoscale. To demonstrate the power of this new technology, our interdisciplinary team will probe the important biological problems of amyloid aggregation and tumor suppressor p53 protein folding dynamics at high concentrations. The TryptoBoost approach is directly applicable to any protein containing aromatic amino acids. This condition is met by more than 90% of human proteins, so the project breakthroughs will benefit a broad range of biophysical, chemical, and medical applications. For instance, it will improve the development of therapeutic drugs, increase the detection sensitivity and read-out speed in analytical biosensing on chip, and provide new nanostructures to enhance ultraviolet photocatalysis.

 Publications

year authors and title journal last update
List of publications.
2019 Quanbo Jiang, Benoît Rogez, Jean-Benoît Claude, Guillaume Baffou, Jérôme Wenger
Temperature Measurement in Plasmonic Nanoapertures Used for Optical Trapping
published pages: 1763-1773, ISSN: 2330-4022, DOI: 10.1021/acsphotonics.9b00519
ACS Photonics 6/7 2020-04-24
2020 Satyajit Patra, Mikhail Baibakov, Jean-Benoît Claude, Jérôme Wenger
Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels
published pages: 5235, ISSN: 2045-2322, DOI: 10.1038/s41598-020-61856-9
Scientific Reports 10/1 2020-04-24
2020 Aleksandr Barulin, Jérôme Wenger
Ultraviolet Photostability Improvement for Autofluorescence Correlation Spectroscopy on Label-Free Proteins
published pages: 2027-2035, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.0c00209
The Journal of Physical Chemistry Letters 11/6 2020-04-24
2020 Mikhail Baibakov, Satyajit Patra, Jean-Benoît Claude, Jérôme Wenger
Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides
published pages: 6947-6955, ISSN: 2470-1343, DOI: 10.1021/acsomega.0c00322
ACS Omega 5/12 2020-04-24
2019 Aleksandr Barulin, Jean-Benoît Claude, Satyajit Patra, Antonin Moreau, Julien Lumeau, Jérôme Wenger
Preventing Aluminum Photocorrosion for Ultraviolet Plasmonics
published pages: 5700-5707, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.9b02137
The Journal of Physical Chemistry Letters 10/19 2020-04-24
2020 Quanbo Jiang, Benoît Rogez, Jean-Benoît Claude, Antonin Moreau, Julien Lumeau, Guillaume Baffou, Jérôme Wenger
Adhesion layer influence on controlling the local temperature in plasmonic gold nanoholes
published pages: 2524-2531, ISSN: 2040-3364, DOI: 10.1039/c9nr08113e
Nanoscale 12/4 2020-04-24
2019 Aleksandr Barulin, Jean-Benoît Claude, Satyajit Patra, Nicolas Bonod, Jérôme Wenger
Deep Ultraviolet Plasmonic Enhancement of Single Protein Autofluorescence in Zero-Mode Waveguides
published pages: 7434-7442, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.9b03137
Nano Letters 19/10 2020-04-24
2020 Abdennacer Benali, Jean-Benoît Claude, Nicoletta Granchi, Simona Checcucci, Mohammed Bouabdellaoui, Mimoun Zazoui, Monica Bollani, Marco Salvalaglio, Jérôme Wenger, Luc Favre, David Grosso, Antoine Ronda, Isabelle Berbezier, Massimo Gurioli, Marco Abbarchi
Flexible photonic devices based on dielectric antennas
published pages: 15002, ISSN: 2515-7647, DOI: 10.1088/2515-7647/ab6713
Journal of Physics: Photonics 2/1 2020-04-24
2019 Vamsi K. Moparthi, Satish B. Moparthi, Christoph Howe, Patrícia Raleiras, Jerome Wenger, Karin Stensjö
Structural diffusion properties of two atypical Dps from the cyanobacterium Nostoc punctiforme disclose interactions with ferredoxins and DNA
published pages: 148063, ISSN: 0005-2728, DOI: 10.1016/j.bbabio.2019.148063
Biochimica et Biophysica Acta (BBA) - Bioenergetics 1860/10 2020-04-24
2019 Mikhail Baibakov, Satyajit Patra, Jean-Benoît Claude, Antonin Moreau, Julien Lumeau, Jérôme Wenger
Extending Single-Molecule Förster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides
published pages: 8469-8480, ISSN: 1936-0851, DOI: 10.1021/acsnano.9b04378
ACS Nano 13/7 2020-04-24

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