Opendata, web and dolomites


Single-molecule spectroscopy of coordinated motions in allosteric proteins

Total Cost €


EC-Contrib. €






Project "SMALLOSTERY" data sheet

The following table provides information about the project.


Organization address
address: HERZL STREET 234
postcode: 7610001

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Israel [IL]
 Total cost 2˙484˙722 €
 EC max contribution 2˙484˙722 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2022-04-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WEIZMANN INSTITUTE OF SCIENCE IL (REHOVOT) coordinator 2˙484˙722.00


 Project objective

Critical for the function of many proteins, allosteric communication involves transmission of the effect of binding at one site of a protein to another through conformational changes. Yet the structural and dynamic basis for allostery remains poorly understood. In particular, there is no method to follow coordinated large-scale motions of domains and subunits in proteins as they occur. Since the subunits of allosteric proteins often contain multiple domains, any such method entails probing the dynamics along several intra-protein distances simultaneously. This proposal aims at ameliorating this deficiency by creating the experimental framework for exploring time-dependent coordination of allosteric transitions of multiple units within proteins. Our methodology will rely on single-molecule FRET spectroscopy with multiple labels on the same protein and advanced analysis. We will explore fundamental issues in protein dynamics: relative motions of domains within subunits, propagation of conformational change between subunits, and synchronization of these motions by effector molecules. To investigate these issues, we have carefully selected three model systems, each representing an important scenario of allosteric regulation. While the homo-oligomeric protein-folder GroEL conserves symmetry in a concerted transition between major structural states, the symmetry of the homo-oligomeric disaggregating machine ClpB is broken via a sequential transition. Symmetry is attained only after binding to DNA and ligands in the third system, the family of RXR heterodimers. This exciting project will provide the very first catalogue of coordinated and time-ordered motions within and between subunits of allosteric proteins and the first measurement of the time scale of the conformational spread through a large protein. It will enhance dramatically our understanding of how allostery contributes to protein function, influencing future efforts to design drugs for allosteric proteins.


year authors and title journal last update
List of publications.
2019 Hisham Mazal, Gilad Haran
Single-molecule FRET methods to study the dynamics of proteins at work
published pages: 8-17, ISSN: 2468-4511, DOI: 10.1016/j.cobme.2019.08.007
Current Opinion in Biomedical Engineering 12 2020-02-06
2019 Hisham Mazal, Marija Iljina, Yoav Barak, Nadav Elad, Rina Rosenzweig, Pierre Goloubinoff, Inbal Riven, Gilad Haran
Tunable microsecond dynamics of an allosteric switch regulate the activity of a AAA+ disaggregation machine
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09474-6
Nature Communications 10/1 2020-01-29
2018 Haim Yuval Aviram, Menahem Pirchi, Hisham Mazal, Yoav Barak, Inbal Riven, Gilad Haran
Direct observation of ultrafast large-scale dynamics of an enzyme under turnover conditions
published pages: 3243-3248, ISSN: 0027-8424, DOI: 10.1073/pnas.1720448115
Proceedings of the National Academy of Sciences 115/13 2019-10-08

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SMALLOSTERY" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "SMALLOSTERY" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

InsideChromatin (2019)

Towards Realistic Modelling of Nucleosome Organization Inside Functional Chromatin Domains

Read More  

SuperH (2019)

Discovery and Characterization of Hydrogen-Based High-Temperature Superconductors

Read More  


The Enemy of the Good: Towards a Theory of Moral Progress

Read More