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SPOCkS MS SIGNED

Sampling Protein cOmplex Conformational Space with native top down Mass Spectrometry

Total Cost €

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EC-Contrib. €

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Partnership

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 SPOCkS MS project word cloud

Explore the words cloud of the SPOCkS MS project. It provides you a very rough idea of what is the project "SPOCkS MS" about.

species    employed    pipeline    pathogenic    positions    artefacts    report    exposed    versatile    transcription    dissociation    space    models    local    deuterium    replication    fragmentation    constraints    time    resolved    separation    mass    resist    sequence    coverage    combined    selective    complemented    model    strategies    conversion    violet    conformation    solution    ultra    area    accessible    coronaviral    additional    structure    bulk    electron    transient    happens    ms    integrate    software    spectrometry    conformational    barely    flexible    structures    fed    maximise    newly    intracellular    techniques    averaging    question    free    biology    content    highest    conventional    structural    exchange    data    substrates    fragment    lasers    protein    hydrogen    hydroxyl    derive    sampling    crystallisation    suite    viruses    avoiding    remedy    proteins    complexes    surface    interfaces    labelling    stages    read       viral    spock    human    polyproteins    footprinting    native    combine    atomistic    adapts    phosphorylation    nature    samples    gas   

Project "SPOCkS MS" data sheet

The following table provides information about the project.

Coordinator
HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE 

Organization address
address: MARTINISTRASSE 52
city: HAMBURG
postcode: 20251
website: www.hpi-hamburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙999˙000 €
 EC max contribution 1˙999˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE DE (HAMBURG) coordinator 1˙999˙000.00

Map

 Project objective

The main question to be addressed by SPOCk’S MS is how protein complex conformation adapts to local changes, such as processing of polyproteins, protein phosphorylation or conversion of substrates. While labelling strategies combined with mass spectrometry (MS), such as hydrogen deuterium exchange and hydroxyl footprinting, are very versatile in studying protein structure, these techniques are employed on bulk samples averaging over all species present. SPOCk’S MS will remedy these by studying the footprinting and therefore exposed surface area on conformation and mass selected species. Labelling still happens in solution avoiding gas phase associated artefacts. The labelling positions are then read out using newly developed top-down MS technology. Ultra-violet and free-electron lasers will be employed to fragment the protein complexes in the gas phase. In order to achieve the highest possible sequence and thus structural coverage, lasers will be complemented by additional dissociation and separation stages to allow MS^N. SPOCk’S MS will allow sampling conformational space of proteins and protein complexes and especially report about the transient nature of protein interfaces. Constraints derived in MS will be fed into a dedicated software pipeline to derive atomistic models. SPOCk’S MS will be used to study intracellular viral protein complexes, especially coronaviral replication/transcription complexes, which are highly flexible and often resist crystallisation and are barely accessible by conventional structural biology techniques. Objectives: - Integrate labelling with complex species selective native MS for time-resolved structural studies - Combine fragmentation techniques to maximise information content from MS - Develop software suite to analyse data and model protein complex structures based on MS constraints - Apply SPOCk’S MS to protein complexes of human pathogenic viruses

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The information about "SPOCKS MS" are provided by the European Opendata Portal: CORDIS opendata.

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