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SPOCkS MS SIGNED

Sampling Protein cOmplex Conformational Space with native top down Mass Spectrometry

Total Cost €

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EC-Contrib. €

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Partnership

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 SPOCkS MS project word cloud

Explore the words cloud of the SPOCkS MS project. It provides you a very rough idea of what is the project "SPOCkS MS" about.

hydrogen    transcription    ms    selective    replication    newly    report    viral    mass    sampling    human    happens    complexes    exchange    samples    coverage    structural    question    gas    barely    techniques    bulk    adapts    spectrometry    separation    conversion    polyproteins    violet    species    substrates    spock    flexible    sequence    conformation    suite    model    models       labelling    software    fed    conformational    space    phosphorylation    pipeline    crystallisation    fragmentation    interfaces    resist    local    surface    lasers    native    solution    data    strategies    transient    resolved    viruses    integrate    exposed    conventional    deuterium    content    hydroxyl    stages    intracellular    proteins    accessible    additional    electron    protein    area    remedy    highest    versatile    avoiding    employed    positions    pathogenic    fragment    time    complemented    structure    averaging    nature    artefacts    footprinting    coronaviral    derive    structures    biology    maximise    ultra    dissociation    read    atomistic    combine    constraints    combined    free   

Project "SPOCkS MS" data sheet

The following table provides information about the project.

Coordinator
HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE 

Organization address
address: MARTINISTRASSE 52
city: HAMBURG
postcode: 20251
website: www.hpi-hamburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙999˙000 €
 EC max contribution 1˙999˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE DE (HAMBURG) coordinator 1˙999˙000.00

Map

 Project objective

The main question to be addressed by SPOCk’S MS is how protein complex conformation adapts to local changes, such as processing of polyproteins, protein phosphorylation or conversion of substrates. While labelling strategies combined with mass spectrometry (MS), such as hydrogen deuterium exchange and hydroxyl footprinting, are very versatile in studying protein structure, these techniques are employed on bulk samples averaging over all species present. SPOCk’S MS will remedy these by studying the footprinting and therefore exposed surface area on conformation and mass selected species. Labelling still happens in solution avoiding gas phase associated artefacts. The labelling positions are then read out using newly developed top-down MS technology. Ultra-violet and free-electron lasers will be employed to fragment the protein complexes in the gas phase. In order to achieve the highest possible sequence and thus structural coverage, lasers will be complemented by additional dissociation and separation stages to allow MS^N. SPOCk’S MS will allow sampling conformational space of proteins and protein complexes and especially report about the transient nature of protein interfaces. Constraints derived in MS will be fed into a dedicated software pipeline to derive atomistic models. SPOCk’S MS will be used to study intracellular viral protein complexes, especially coronaviral replication/transcription complexes, which are highly flexible and often resist crystallisation and are barely accessible by conventional structural biology techniques. Objectives: - Integrate labelling with complex species selective native MS for time-resolved structural studies - Combine fragmentation techniques to maximise information content from MS - Develop software suite to analyse data and model protein complex structures based on MS constraints - Apply SPOCk’S MS to protein complexes of human pathogenic viruses

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The information about "SPOCKS MS" are provided by the European Opendata Portal: CORDIS opendata.

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