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SPOCkS MS SIGNED

Sampling Protein cOmplex Conformational Space with native top down Mass Spectrometry

Total Cost €

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EC-Contrib. €

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Partnership

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 SPOCkS MS project word cloud

Explore the words cloud of the SPOCkS MS project. It provides you a very rough idea of what is the project "SPOCkS MS" about.

avoiding    read    software    adapts    content    stages    replication    maximise    spectrometry    viruses    nature    violet    exposed    fragment    human    remedy    gas    report    ultra    transcription    accessible    fragmentation    interfaces    protein    footprinting    hydrogen       electron    highest    structural    phosphorylation    question    species    surface    resist    flexible    viral    data    coverage    exchange    combine    complexes    versatile    intracellular    resolved    barely    deuterium    substrates    fed    conversion    separation    pathogenic    averaging    polyproteins    strategies    labelling    structures    time    model    selective    complemented    suite    conventional    solution    happens    employed    crystallisation    atomistic    local    conformational    biology    structure    transient    conformation    samples    spock    additional    free    native    combined    coronaviral    hydroxyl    pipeline    newly    lasers    constraints    bulk    space    artefacts    models    positions    proteins    derive    techniques    sequence    sampling    ms    area    mass    integrate    dissociation   

Project "SPOCkS MS" data sheet

The following table provides information about the project.

Coordinator
HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE 

Organization address
address: MARTINISTRASSE 52
city: HAMBURG
postcode: 20251
website: www.hpi-hamburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙999˙000 €
 EC max contribution 1˙999˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE DE (HAMBURG) coordinator 1˙999˙000.00

Map

 Project objective

The main question to be addressed by SPOCk’S MS is how protein complex conformation adapts to local changes, such as processing of polyproteins, protein phosphorylation or conversion of substrates. While labelling strategies combined with mass spectrometry (MS), such as hydrogen deuterium exchange and hydroxyl footprinting, are very versatile in studying protein structure, these techniques are employed on bulk samples averaging over all species present. SPOCk’S MS will remedy these by studying the footprinting and therefore exposed surface area on conformation and mass selected species. Labelling still happens in solution avoiding gas phase associated artefacts. The labelling positions are then read out using newly developed top-down MS technology. Ultra-violet and free-electron lasers will be employed to fragment the protein complexes in the gas phase. In order to achieve the highest possible sequence and thus structural coverage, lasers will be complemented by additional dissociation and separation stages to allow MS^N. SPOCk’S MS will allow sampling conformational space of proteins and protein complexes and especially report about the transient nature of protein interfaces. Constraints derived in MS will be fed into a dedicated software pipeline to derive atomistic models. SPOCk’S MS will be used to study intracellular viral protein complexes, especially coronaviral replication/transcription complexes, which are highly flexible and often resist crystallisation and are barely accessible by conventional structural biology techniques. Objectives: - Integrate labelling with complex species selective native MS for time-resolved structural studies - Combine fragmentation techniques to maximise information content from MS - Develop software suite to analyse data and model protein complex structures based on MS constraints - Apply SPOCk’S MS to protein complexes of human pathogenic viruses

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The information about "SPOCKS MS" are provided by the European Opendata Portal: CORDIS opendata.

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