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SPOCkS MS SIGNED

Sampling Protein cOmplex Conformational Space with native top down Mass Spectrometry

Total Cost €

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EC-Contrib. €

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Partnership

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 SPOCkS MS project word cloud

Explore the words cloud of the SPOCkS MS project. It provides you a very rough idea of what is the project "SPOCkS MS" about.

strategies    barely    versatile    additional    sampling    proteins    exposed    selective    crystallisation    conformation    combine    dissociation    transient    software    interfaces    fed    samples    derive    solution    structure    data    artefacts    stages    free    exchange    maximise    electron    coverage    area    accessible    resolved    report    hydrogen    intracellular    hydroxyl    remedy    highest    conventional    viral    local    happens    spock    viruses    conformational    complemented    constraints    suite    replication    surface    pathogenic    labelling    read    flexible    structural    space    separation    transcription    adapts    deuterium    substrates    lasers    native    gas    complexes    species    question    time    protein    positions    model    spectrometry    polyproteins    content    coronaviral    human    newly    conversion    resist    avoiding    ms    combined    integrate    models    employed    fragment       mass    nature    pipeline    footprinting    averaging    ultra    sequence    techniques    atomistic    structures    phosphorylation    fragmentation    violet    bulk    biology   

Project "SPOCkS MS" data sheet

The following table provides information about the project.

Coordinator
HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE 

Organization address
address: MARTINISTRASSE 52
city: HAMBURG
postcode: 20251
website: www.hpi-hamburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙999˙000 €
 EC max contribution 1˙999˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2022-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE DE (HAMBURG) coordinator 1˙999˙000.00

Map

 Project objective

The main question to be addressed by SPOCk’S MS is how protein complex conformation adapts to local changes, such as processing of polyproteins, protein phosphorylation or conversion of substrates. While labelling strategies combined with mass spectrometry (MS), such as hydrogen deuterium exchange and hydroxyl footprinting, are very versatile in studying protein structure, these techniques are employed on bulk samples averaging over all species present. SPOCk’S MS will remedy these by studying the footprinting and therefore exposed surface area on conformation and mass selected species. Labelling still happens in solution avoiding gas phase associated artefacts. The labelling positions are then read out using newly developed top-down MS technology. Ultra-violet and free-electron lasers will be employed to fragment the protein complexes in the gas phase. In order to achieve the highest possible sequence and thus structural coverage, lasers will be complemented by additional dissociation and separation stages to allow MS^N. SPOCk’S MS will allow sampling conformational space of proteins and protein complexes and especially report about the transient nature of protein interfaces. Constraints derived in MS will be fed into a dedicated software pipeline to derive atomistic models. SPOCk’S MS will be used to study intracellular viral protein complexes, especially coronaviral replication/transcription complexes, which are highly flexible and often resist crystallisation and are barely accessible by conventional structural biology techniques. Objectives: - Integrate labelling with complex species selective native MS for time-resolved structural studies - Combine fragmentation techniques to maximise information content from MS - Develop software suite to analyse data and model protein complex structures based on MS constraints - Apply SPOCk’S MS to protein complexes of human pathogenic viruses

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The information about "SPOCKS MS" are provided by the European Opendata Portal: CORDIS opendata.

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