Explore the words cloud of the NucLoc project. It provides you a very rough idea of what is the project "NucLoc" about.
The following table provides information about the project.
UNIVERSITEIT VAN AMSTERDAM
|Coordinator Country||Netherlands [NL]|
|Total cost||165˙598 €|
|EC max contribution||165˙598 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2018-09-01 to 2020-08-31|
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|1||UNIVERSITEIT VAN AMSTERDAM||NL (AMSTERDAM)||coordinator||165˙598.00|
In eukaryotic cells mRNAs can be localized to specific places within a cell to achieve protein synthesis with spatiotemporal control. Recently, I and others have shown that prokaryotes can also target mRNAs to specific parts of the cell. However, these studies have been restricted to a limited number of mRNAs and were performed with Gram-negative bacteria, and due to the serendipitous nature of these discoveries and conflicting results, it is not clear how widespread mRNA localization is in a bacterial cell and what conserved mechanisms are playing a role. To investigate this, I will first employ a novel CLIP-seq-inspired deep-sequencing-based approach to gain an unbiased view of mRNAs enriched at the cell periphery and cell poles of the Gram-positive model bacterium Bacillus subtilis. This dataset will reveal the scope of mRNA targeting by the Signal Recognition Particle (SRP) and will reveal mRNAs that use another mechanism for cellular targeting. In addition, classic CLIP-seq experiments will be used to identify mRNAs that use the cytoskeleton and other morphological proteins for targeting. As a complementary approach, I will assess the localization of mRNAs coding for different classes of localized proteins (polar, cell division, cytoskeletal, membrane, secreted, motility, nucleoid and chaperone proteins) using the established fluorescence in situ hybridization (FISH) technique. Information obtained from these experiments will subsequently be used to investigate the role of protein translation, RNA sequences, genome location and cell morphology in mRNA localization, to gain functional insight into the mechanisms responsible for this basic morphogenetic process.
|year||authors and title||journal||last update|
Declan A. Gray, Gaurav Dugar, Pamela Gamba, Henrik Strahl, Martijs J. Jonker, Leendert W. Hamoen
Extreme slow growth as alternative strategy to survive deep starvation in bacteria
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-08719-8
|Nature Communications 10/1||2020-04-08|
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The information about "NUCLOC" are provided by the European Opendata Portal: CORDIS opendata.
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