Opendata, web and dolomites

TiPS-DNA SIGNED

Transcription-induced Plectonemic Supercoiled DNA

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

Project "TiPS-DNA" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 165˙598 €
 EC max contribution 165˙598 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-03-01   to  2020-02-29

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 165˙598.00

Map

 Project objective

In our cells, DNA is extremely highly compacted to fit within the cell’s nucleus. While being compacted, the DNA still needs to remain accessible to various processes such as gene expression during the cell cycle. So how is DNA spatially organized? How does this compacted DNA interact with enzymes? Does compacted DNA superstructure have a biological role? Answering these daunting biological riddles through a targeted physics approach is the central motive of this proposal. I aim to investigate how supercoiled DNA plectonemes, the basic unit of DNA superstructure found in chromosomes, is linked to its biological functions in gene regulation. Throughout the proposed biophysics experiments in this project, I aim to obtain direct experimental proofs that reveal the mechanism behind the transcription-induced DNA supercoiling. I will co-visualize RNA polymerase interacting with supercoiled DNA in real-time and study the relevant dynamics during complex formation and transcription. To this end I will employ a novel single molecule in-vitro assay, Intercalation-induced Supercoiling of DNA. This assay utilizes intercalating dyes to induce supercoiling as well as to visualize the formation of DNA-plectonemes. The technique is solely based on fluorescence microscopy and allows for tracking DNA plectonemes and labelled enzymes with ease and with high-throughput. This project will provide the first direct evidence on the role of DNA supercoiling – a fundamental yet not-well studied phenomenon in cell biology – in mediating the activity of DNA-binding enzymes as well as on the origins of dynamic DNA supercoiling in the topological domains of the genome. The result of this project will be crucial for the understanding of the physical interactions that couple chromosome organisation to gene regulation.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TIPS-DNA" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TIPS-DNA" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

NaWaTL (2020)

Narrative, Writing, and the Teotihuacan Language: Exploring Language History Through Phylogenetics, Epigraphy and Iconography

Read More  

MIGPSC (2018)

Shaping the European Migration Policy: the role of the security industry

Read More  

RESTRICTIONAPP (2019)

A multilinear approach to the restriction problem with applications to geometric measure theory, the Schrödinger equation and inverse problems

Read More