Opendata, web and dolomites

Nedd8Activate SIGNED

How does the ubiquitin-like protein NEDD8 activate ubiquitin ligase machineries?

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 Nedd8Activate project word cloud

Explore the words cloud of the Nedd8Activate project. It provides you a very rough idea of what is the project "Nedd8Activate" about.

proteins    enzymes    nearly    ubls    elusive    visualize    cells    chemical    families    class    substrates    disease    reactions    purify    domains    discover    activated    cullin    little    atypical    family    giant    regulatory    detect    generate    monoubiquitylation    goals    biochemical    track    tour    rbr    crls    paradigms    regulate    polyubiquitin    resource    nedd8    cell    ligases    modification    post    structures    mark    activating    capture    ubl    mediate    temporally    action    ubiquitylating    first    molecular    e3    functions    peculiar    devise    fraction    biology    form    act    mechanism    modifications    eukaryotic    half    discovered    transform    comprehensively    ring    ligase    nonetheless    58    neddylated    structural    mechanisms    regulates    tools    interacting    force    translational    assemblies    forms    ariadne    multiple    subunits    label    de    affinity    fleeting    identical    reagents    biological    ubiquitin    ubiquitins    activates    chains    e3s    cryo    monoubiquitin    regulation    interactions    em   

Project "Nedd8Activate" data sheet

The following table provides information about the project.

Coordinator
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV 

Organization address
address: HOFGARTENSTRASSE 8
city: Munich
postcode: 80539
website: www.mpg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙193˙871 €
 EC max contribution 2˙193˙871 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-10-01   to  2023-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (Munich) coordinator 2˙193˙871.00

Map

 Project objective

Post-translational modification by ubiquitin and ubiquitin-like proteins (UBLs) is a major eukaryotic regulatory mechanism. Nonetheless, we have little understanding of the detailed mechanisms by which most E3 ligases mark specific targets with monoubiquitin, multiple ubiquitins or specific polyubiquitin chains, or of how UBL modifications transform the functions of their targets. This proposal addresses both problems. First, we will discover the structural mechanisms by which the UBL NEDD8 (58% identical to ubiquitin) activates numerous distinct functions of its targets, which are cullin subunits of cullin-RING E3 ubiquitin ligases (CRLs). Second, we will take a tour-de-force structural, biochemical, and molecular cell biological approach to determine how NEDD8-activated E3 ligases regulate their substrates. Because CRLs form nearly half of all E3 ligases, and as we recently discovered, neddylated CRLs act in part by activating monoubiquitylation by another family of E3 ligases (Ariadne-family RBR E3s), the proposed studies will establish paradigms for a major fraction of ubiquitylating enzymes. To achieve these goals, we will devise novel chemical biology tools to capture fleeting assemblies that typically only occur during chemical reactions, and visualize structures of neddylated CRLs “in action” by cryo EM. We will generate a resource of novel reagents that detect, label, and affinity purify activated forms of E3 ligases to temporally track their interactions during pathways they regulate in cells. And we will define the mechanisms and structures of a class of atypical, disease-associated giant E3 ligases whose domains and interacting partners are so peculiar that their activities remain elusive. Overall, we will comprehensively define how a UBL directly regulates its targets, and how two major E3 ligase families mediate regulation.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NEDD8ACTIVATE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NEDD8ACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

SLAMseq (2019)

SLAMseq: Temporal resolution in gene expression profiling across multiple platforms

Read More  

ENTRAPMENT (2019)

Septins: from bacterial entrapment to cellular immunity

Read More  

EVOCELFATE (2019)

Evolution of cell fate specification modes in spiral cleavage

Read More