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nanoEx SIGNED

Towards nanopore proteomics: enhancing cytolysin performance through genetically encoded noncanonical amino acids

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 nanoEx project word cloud

Explore the words cloud of the nanoEx project. It provides you a very rough idea of what is the project "nanoEx" about.

noncanonical    biosensing    engineer    transfer    minion    propensity    proteins    species    accurate    ncaa    rna    with    escape    anticipate    creation    amino    abundance    samples    genetic    helical    markers    led    site    sequencing    alpha    combines    thaw    analogous    mitigated    structural    stages    variants    containing    expertise    employ    lysenin    gce    dna    biophysical    handling    treatments    pores    industry    nanoex    walls    freeze    lining    relies    incorporation    appropriate    denature    fixation    covalently    nanopores    employability    protein    biosensors    modulate    residues    instability    expansion    actinoporin    evolution    sensing    pore    architectures    acid    channel    nanopore    multidisciplinary    detection    techniques    progress    identification    actions    beta    tend    seek    directed    ecological    patients    barrel    clinical    invasive    stabilize    inherent    academic    action    code    executed    biomedical    characterization    career    competitiveness    smaller    region    biological    cytolysins    host    training   

Project "nanoEx" data sheet

The following table provides information about the project.

Coordinator
KEMIJSKI INSTITUT 

Organization address
address: HAJDRIHOVA 19
city: LJUBLJANA
postcode: 1000
website: http://www.ki.si

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Slovenia [SI]
 Total cost 162˙040 €
 EC max contribution 162˙040 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KEMIJSKI INSTITUT SI (LJUBLJANA) coordinator 162˙040.00

Map

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 Project objective

Accurate detection of low-abundance proteins in biological samples obtained from patients or invasive species relies on appropriate handling and fixation techniques. Due to their inherent instability and propensity to denature upon freeze-thaw treatments, important protein markers tend to escape detection. Analogous challenges in RNA sequencing have been mitigated by the use of nanopore technology. European academic research and industry actions led to the creation of the widely used MinION devices that employ nanopore technology to enable DNA and RNA sequencing to be executed on site. Through this application I seek to improve nanopores as biosensors for the detection of proteins that will facilitate immediate protein analysis from clinical or ecological samples. I will use my expertise in genetic code expansion (GCE) techniques to engineer two well-characterized cytolysins with distinct architectures: an α-helical actinoporin and β-barrel containing lysenin. To improve these pores for protein identification and sequencing, the proposed research will advance through four stages: Incorporation of noncanonical amino acid (ncAA) to covalently stabilize smaller pores (1) and modulate the pore’s sensing region (2), directed evolution of residues lining the channel walls (3) and structural characterization of the identified variants (4). This is a multidisciplinary project that combines GCE techniques with advanced biophysical and structural characterization of the evolved nanopores. The host’s expertise in nanopore analysis together with my experience with GCE and directed evolution methods provide an important two-way transfer of knowledge. Action includes a training-through-research essential to advance my future academic career and enhance my employability in the biomedical industry. I anticipate the results of nanoEx project will have a major impact on the progress of nanopore biosensing, increasing the competitiveness of the European industry in protein sequencing.

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The information about "NANOEX" are provided by the European Opendata Portal: CORDIS opendata.

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