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nanoEx SIGNED

Towards nanopore proteomics: enhancing cytolysin performance through genetically encoded noncanonical amino acids

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 nanoEx project word cloud

Explore the words cloud of the nanoEx project. It provides you a very rough idea of what is the project "nanoEx" about.

led    barrel    host    stages    stabilize    patients    architectures    lining    analogous    structural    proteins    handling    biosensors    incorporation    residues    mitigated    acid    actions    channel    ncaa    expertise    executed    actinoporin    smaller    nanopore    samples    expansion    career    identification    pores    walls    ecological    fixation    containing    amino    treatments    gce    biosensing    freeze    noncanonical    site    alpha    engineer    pore    genetic    markers    evolution    progress    combines    biophysical    tend    inherent    modulate    relies    directed    propensity    nanopores    protein    variants    with    academic    dna    helical    employability    escape    multidisciplinary    lysenin    minion    accurate    nanoex    covalently    beta    biological    characterization    seek    competitiveness    invasive    sensing    instability    abundance    sequencing    creation    clinical    employ    cytolysins    code    anticipate    rna    techniques    industry    training    species    denature    detection    biomedical    appropriate    transfer    region    thaw    action   

Project "nanoEx" data sheet

The following table provides information about the project.

Coordinator
KEMIJSKI INSTITUT 

Organization address
address: HAJDRIHOVA 19
city: LJUBLJANA
postcode: 1000
website: http://www.ki.si

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Slovenia [SI]
 Total cost 162˙040 €
 EC max contribution 162˙040 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KEMIJSKI INSTITUT SI (LJUBLJANA) coordinator 162˙040.00

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 Project objective

Accurate detection of low-abundance proteins in biological samples obtained from patients or invasive species relies on appropriate handling and fixation techniques. Due to their inherent instability and propensity to denature upon freeze-thaw treatments, important protein markers tend to escape detection. Analogous challenges in RNA sequencing have been mitigated by the use of nanopore technology. European academic research and industry actions led to the creation of the widely used MinION devices that employ nanopore technology to enable DNA and RNA sequencing to be executed on site. Through this application I seek to improve nanopores as biosensors for the detection of proteins that will facilitate immediate protein analysis from clinical or ecological samples. I will use my expertise in genetic code expansion (GCE) techniques to engineer two well-characterized cytolysins with distinct architectures: an α-helical actinoporin and β-barrel containing lysenin. To improve these pores for protein identification and sequencing, the proposed research will advance through four stages: Incorporation of noncanonical amino acid (ncAA) to covalently stabilize smaller pores (1) and modulate the pore’s sensing region (2), directed evolution of residues lining the channel walls (3) and structural characterization of the identified variants (4). This is a multidisciplinary project that combines GCE techniques with advanced biophysical and structural characterization of the evolved nanopores. The host’s expertise in nanopore analysis together with my experience with GCE and directed evolution methods provide an important two-way transfer of knowledge. Action includes a training-through-research essential to advance my future academic career and enhance my employability in the biomedical industry. I anticipate the results of nanoEx project will have a major impact on the progress of nanopore biosensing, increasing the competitiveness of the European industry in protein sequencing.

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The information about "NANOEX" are provided by the European Opendata Portal: CORDIS opendata.

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