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nanoEx SIGNED

Towards nanopore proteomics: enhancing cytolysin performance through genetically encoded noncanonical amino acids

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 nanoEx project word cloud

Explore the words cloud of the nanoEx project. It provides you a very rough idea of what is the project "nanoEx" about.

structural    pore    fixation    lining    actinoporin    instability    biophysical    clinical    markers    mitigated    executed    minion    engineer    multidisciplinary    amino    architectures    employ    alpha    combines    variants    sensing    detection    actions    proteins    progress    expertise    characterization    pores    directed    analogous    stages    genetic    helical    covalently    residues    inherent    rna    career    seek    thaw    tend    transfer    invasive    channel    biomedical    nanoex    biosensors    beta    industry    anticipate    led    noncanonical    smaller    handling    nanopore    acid    abundance    with    relies    biological    patients    code    lysenin    gce    identification    walls    evolution    barrel    stabilize    dna    accurate    expansion    region    creation    treatments    techniques    action    competitiveness    denature    freeze    biosensing    sequencing    propensity    appropriate    protein    site    samples    nanopores    academic    host    incorporation    ecological    ncaa    training    modulate    cytolysins    containing    species    escape    employability   

Project "nanoEx" data sheet

The following table provides information about the project.

Coordinator
KEMIJSKI INSTITUT 

Organization address
address: HAJDRIHOVA 19
city: LJUBLJANA
postcode: 1000
website: http://www.ki.si

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Slovenia [SI]
 Total cost 162˙040 €
 EC max contribution 162˙040 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KEMIJSKI INSTITUT SI (LJUBLJANA) coordinator 162˙040.00

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 Project objective

Accurate detection of low-abundance proteins in biological samples obtained from patients or invasive species relies on appropriate handling and fixation techniques. Due to their inherent instability and propensity to denature upon freeze-thaw treatments, important protein markers tend to escape detection. Analogous challenges in RNA sequencing have been mitigated by the use of nanopore technology. European academic research and industry actions led to the creation of the widely used MinION devices that employ nanopore technology to enable DNA and RNA sequencing to be executed on site. Through this application I seek to improve nanopores as biosensors for the detection of proteins that will facilitate immediate protein analysis from clinical or ecological samples. I will use my expertise in genetic code expansion (GCE) techniques to engineer two well-characterized cytolysins with distinct architectures: an α-helical actinoporin and β-barrel containing lysenin. To improve these pores for protein identification and sequencing, the proposed research will advance through four stages: Incorporation of noncanonical amino acid (ncAA) to covalently stabilize smaller pores (1) and modulate the pore’s sensing region (2), directed evolution of residues lining the channel walls (3) and structural characterization of the identified variants (4). This is a multidisciplinary project that combines GCE techniques with advanced biophysical and structural characterization of the evolved nanopores. The host’s expertise in nanopore analysis together with my experience with GCE and directed evolution methods provide an important two-way transfer of knowledge. Action includes a training-through-research essential to advance my future academic career and enhance my employability in the biomedical industry. I anticipate the results of nanoEx project will have a major impact on the progress of nanopore biosensing, increasing the competitiveness of the European industry in protein sequencing.

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The information about "NANOEX" are provided by the European Opendata Portal: CORDIS opendata.

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