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FuncDis3D SIGNED

Deconstructing gene regulation through functional dissection of the 3D genome

Total Cost €

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EC-Contrib. €

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Partnership

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Project "FuncDis3D" data sheet

The following table provides information about the project.

Coordinator
STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS 

Organization address
address: PLESMANLAAN 121
city: AMSTERDAM
postcode: 1066 CX
website: www.nki.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙999˙925 €
 EC max contribution 1˙997˙425 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2019-COG
 Funding Scheme ERC-COG
 Starting year 2020
 Duration (year-month-day) from 2020-09-01   to  2025-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    STICHTING HET NEDERLANDS KANKER INSTITUUT-ANTONI VAN LEEUWENHOEK ZIEKENHUIS NL (AMSTERDAM) coordinator 1˙997˙425.00

Map

 Project objective

The three-dimensional organization of the genome inside the nucleus (3DG) is crucial for gene regulation. Although the fundamental building blocks of the 3DG have been identified, how they contribute to gene regulation is incompletely understood. In recent years my lab has started to functionally dissect the 3DG in order to understand the effects on gene expression. The current proposal intends to expand this work and aims to i) broaden the array of proteins that are known organize the 3DG, ii) determine how loss of these proteins affects the 3DG and iii) elucidate how this influences gene expression. The key to this is to induce changes in the 3DG by acute protein depletion and measuring changes over time. We have preliminary data showing that by depleting regulators of the 3DG we can induce changes within a few hours. We will deplete a range of proteins in pluripotent and differentiated cells to determine their contribution to 3DG organization. Simultaneous characterization of the epigenome and transcriptome will allow us to determine the sequence of changes. In addition, we will perform Thousands of Reporters Integrated in Parallel (TRIP) after perturbation of the 3DG. TRIP reporters all have the same minimal promoter that acts as a sensor for promoter-enhancer (mis)-communication. By combining TRIP with 4C we can measure the changes in the reporter-enhancer interaction landscape. Our data will generate fundamental insight into the parameters that govern the interactions between promoters and regulatory elements, within the context of the 3DG, and their effects on gene expression. Furthermore, it allows us to determine the temporal order of changes in the 3DG, the epigenome and gene expression, which is essential for the establishment of cause-and-effect relationships. Collectively, our data will lead to a better appreciation of how distal and proximal regulatory regions cooperate to establish cell-type specific gene expression programs.

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The information about "FUNCDIS3D" are provided by the European Opendata Portal: CORDIS opendata.

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lastchecktime (2020-11-30 4:37:58) correctly updated