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UTPE PEB SIGNED

Ultrahigh-throughput protein evolution for polyethylene biodegradation

Total Cost €

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EC-Contrib. €

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Partnership

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 UTPE PEB project word cloud

Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.

sme    overcome    undertaken    university    desired    slow    rates    pressing    concentration    plastic    associate    ucl    synergize    droplet    degrading    cytometry    environmental    personal    first    ebi    secondments    screens    degradation    enzymes    chassis    cover    mutagenesis    sequencing    directed    library    sequence    persist    enzyme    evolutionarily    biotechnology    scattering    differential    polymer    optimise    oil    genetically    tech    dr    genotype    cambridge    multidisciplinary    degraded    rate    microbiology    measuring    modern    tractable    pe    generation    evolution    effort    flow    assay    phenotype    microfluidics    genotypes    sufficiently    century    group    picoliter    pollution    express    assays    extensive    protein    practical    solution    skills    synthetic    efficiency    droplets    standard    bioinformatics    water    throughput    polyethylene    biochemistry    biodegradation    isolate    strains    sized    convey    functionally    techniques    nature    facilities    light    microbial    drop    molecular    ways    ultrahigh    inefficient    hollfelder    random    abundantly    space    host    waste   

Project "UTPE PEB" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 224˙933 €
 EC max contribution 224˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-08-01   to  2022-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 224˙933.00

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 Project objective

The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.

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