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UTPE PEB SIGNED

Ultrahigh-throughput protein evolution for polyethylene biodegradation

Total Cost €

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EC-Contrib. €

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Partnership

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 UTPE PEB project word cloud

Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.

microbial    group    sme    rates    century    sized    overcome    bioinformatics    genotypes    convey    effort    strains    practical    generation    sequence    degraded    ultrahigh    random    mutagenesis    ucl    host    cytometry    microbiology    biochemistry    modern    multidisciplinary    solution    slow    express    pressing    directed    polyethylene    ebi    evolutionarily    skills    hollfelder    associate    persist    genetically    throughput    extensive    evolution    concentration    degradation    microfluidics    synergize    undertaken    waste    synthetic    functionally    flow    sufficiently    space    first    pe    desired    genotype    phenotype    standard    nature    rate    dr    library    degrading    screens    biodegradation    drop    oil    water    tractable    cambridge    abundantly    chassis    pollution    optimise    scattering    techniques    personal    protein    droplet    biotechnology    sequencing    cover    tech    facilities    environmental    differential    isolate    light    polymer    efficiency    inefficient    measuring    molecular    assay    ways    enzymes    plastic    picoliter    secondments    university    droplets    assays    enzyme   

Project "UTPE PEB" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 224˙933 €
 EC max contribution 224˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-08-01   to  2022-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 224˙933.00

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 Project objective

The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.

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The information about "UTPE PEB" are provided by the European Opendata Portal: CORDIS opendata.

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