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UTPE PEB SIGNED

Ultrahigh-throughput protein evolution for polyethylene biodegradation

Total Cost €

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EC-Contrib. €

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Partnership

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 UTPE PEB project word cloud

Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.

degrading    desired    droplet    modern    abundantly    picoliter    measuring    convey    personal    environmental    undertaken    group    evolutionarily    enzymes    hollfelder    light    genotype    standard    synergize    secondments    chassis    degradation    cover    sequencing    sme    phenotype    express    pressing    biotechnology    waste    polyethylene    library    biochemistry    multidisciplinary    skills    directed    efficiency    ways    assays    pe    century    random    effort    microfluidics    rate    isolate    rates    mutagenesis    protein    plastic    differential    bioinformatics    university    microbial    polymer    practical    droplets    pollution    strains    oil    enzyme    inefficient    ultrahigh    throughput    genetically    optimise    generation    host    water    sized    ucl    slow    flow    first    tractable    synthetic    dr    associate    molecular    drop    cytometry    degraded    ebi    facilities    biodegradation    sequence    solution    nature    microbiology    functionally    space    concentration    overcome    evolution    sufficiently    techniques    persist    cambridge    scattering    extensive    tech    screens    genotypes    assay   

Project "UTPE PEB" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 224˙933 €
 EC max contribution 224˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-08-01   to  2022-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 224˙933.00

Map

 Project objective

The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.

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