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UTPE PEB SIGNED

Ultrahigh-throughput protein evolution for polyethylene biodegradation

Total Cost €

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EC-Contrib. €

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Partnership

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 UTPE PEB project word cloud

Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.

isolate    chassis    tech    picoliter    techniques    degraded    hollfelder    cover    slow    rate    pe    optimise    dr    throughput    convey    screens    random    host    drop    droplet    secondments    measuring    library    functionally    evolutionarily    rates    multidisciplinary    first    droplets    microbial    molecular    microfluidics    mutagenesis    cytometry    environmental    nature    synergize    polymer    associate    water    group    desired    efficiency    biochemistry    standard    genetically    inefficient    solution    genotype    overcome    generation    university    undertaken    cambridge    strains    concentration    evolution    effort    pressing    facilities    scattering    century    tractable    sized    enzymes    abundantly    sequence    biodegradation    degradation    microbiology    ways    extensive    ucl    sufficiently    skills    pollution    sme    practical    protein    synthetic    polyethylene    directed    degrading    plastic    phenotype    space    assay    ultrahigh    assays    light    modern    oil    express    biotechnology    personal    ebi    flow    enzyme    waste    genotypes    differential    persist    sequencing    bioinformatics   

Project "UTPE PEB" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 224˙933 €
 EC max contribution 224˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2020
 Duration (year-month-day) from 2020-08-01   to  2022-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 224˙933.00

Map

 Project objective

The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.

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