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TARGETBINDER

Target binders

Coordinatore	Karlsruher Institut fuer Technologie Organization address address: Kaiserstrasse 12 city: Karlsruhe postcode: 76131 contact info Titolo: Ms. Nome: Natascha Cognome: Wallburg Email: send email Telefono: +49 721 608 25414 Fax: +49 721 608 25403
Nazionalità Coordinatore	Germany [DE]
Totale costo	5˙553˙931 €
EC contributo	3˙988˙730 €
Programma	FP7-HEALTH Specific Programme "Cooperation": Health
Code Call	FP7-HEALTH-2011-two-stage
Funding Scheme	CP-FP
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-01-01 - 2014-12-31

Partecipanti

#	participant	country	role	EC contrib. [€]
1	Karlsruher Institut fuer Technologie Organization address address: Kaiserstrasse 12 city: Karlsruhe postcode: 76131 contact info Titolo: Ms. Nome: Natascha Cognome: Wallburg Email: send email Telefono: +49 721 608 25414 Fax: +49 721 608 25403	DE (Karlsruhe)	coordinator	609˙050.00
2	ISOGENICA LTD Organization address address: "The MANSION, CHESTERFORD RESEARCH PARK" city: LITTLE CHESTERFORD SAFFRON WALDEN postcode: CB10 1XL contact info Titolo: Dr. Nome: Chris Cognome: Ullman Email: send email Telefono: 441800000000 Fax: 441800000000	UK (LITTLE CHESTERFORD SAFFRON WALDEN)	participant	700˙500.00
3	Center for Advanced Bioanalysis GmbH Organization address address: Scharitzerstrasse 6-8 city: Linz postcode: 4020 contact info Titolo: Dr. Nome: Alois Cognome: Sonnleitner Email: send email Telefono: +43 732 606079 16	AT (Linz)	participant	549˙000.00
4	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Organization address address: University Offices, Wellington Square city: OXFORD postcode: OX1 2JD contact info Titolo: Dr. Nome: Stephen Cognome: Conway Email: send email Telefono: +44 1865 289811 Fax: +44 1865 289801	UK (OXFORD)	participant	499˙980.00
5	BIAMETRICS GMBH Organization address address: AUF DER MORGENSTELLE 18 city: TUBINGEN postcode: 72076 contact info Titolo: Dr. Nome: Guenther Cognome: Proll Email: send email Telefono: +49 7071 29 73048 Fax: +49 7071 29 5772	DE (TUBINGEN)	participant	471˙000.00
6	PEPperPRINT GmbH Organization address address: Schlossweg 9 city: Wiesloch postcode: 69168 contact info Titolo: Dr. Nome: Volker Cognome: Stadler Email: send email Telefono: +49 6221 72644 88 Fax: +49 6221 72644 75	DE (Wiesloch)	participant	459˙000.00
7	APOGENIX GMBH Organization address address: IM NEUENHEIMER FELD 584 city: HEIDELBERG postcode: 69120 contact info Titolo: Dr. Nome: Jürgen Cognome: Gamer Email: send email Telefono: +49 6221 586080	DE (HEIDELBERG)	participant	400˙000.00
8	DEUTSCHES KREBSFORSCHUNGSZENTRUM Organization address address: Im Neuenheimer Feld 280 city: HEIDELBERG postcode: 69120 contact info Titolo: Ms. Nome: Bettina Cognome: Crispin Email: send email Telefono: +49 6221 42 2700 Fax: +49 6221 42 2708	DE (HEIDELBERG)	participant	130˙000.00
9	MICROSYSTEMS LTD Organization address address: kv. CHAIKA 15-D-21 city: Varna postcode: 9010 contact info Titolo: Mr. Nome: Nikolay Cognome: Nikolov Email: send email Telefono: +359 52 321998 Fax: +359 52 302534	BG (Varna)	participant	100˙000.00
10	TECHNICAL UNIVERSITY OF VARNA Organization address address: Studentska 1 city: VARNA postcode: 9010 contact info Titolo: Prof. Nome: Jordan Cognome: Kolev Email: send email Telefono: +359 52 302759 Fax: +359 52 302759	BG (VARNA)	participant	70˙200.00

Mappa

Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

apo binders variant throughput dkfz want free procedure protein labelling parallel screens format kit ppp synthesized peptide oxf validate affinity array peptides bia tools binding

Obiettivo del progetto (Objective)

'We want to further develop our tools and technologies for high-throughput research, with the final goals being (I.) the particle-based combinatorial synthesis of 1 Mio different peptides on a glass slide for chemical costs of ~50€ (KIT, CBL, MS, TUV), and (II.) the labelling-free parallel readout of binding affinities by a variant reflectometric interference spectroscopy method for ~10.000 peptide spots per cm(2) when staining the array with an unlabeled protein (BIA, KIT). These tools provide the basis (III.) for a standardized, fast, and reliable high-throughput procedure that we want to develop in order to find high-affinity peptide binders against any pharmaceutically interesting target protein. Such a procedure might have an important impact in medicine and in the biotechnology industry. In order to achieve this goal, we will use display techniques that in combination with high-throughput sequencing typically will identify ~100.000 putative peptide binders per target protein (ISO). These will be synthesized in array format to validate binding to the target protein by an independent method (PPP, DKFZ). Next, based on binders from initial screens, many variant peptides are synthesized in high-density array format for iterative screens (PPP, DKFZ, KIT), whereby massive parallel labelling-free detection of binders pinpoints higher-affinity binders (BIA). In order to validate our novel high-throughput procedure, (IV.) we want to find high-affinity peptide binders against relevant target proteins (delivered by APO and OXF), and test these binders in biological assays (OXF, APO).'

Introduzione (Teaser)

High-throughput screening (HTS) can rapidly identify active compounds for specific targets in drug design, for example. EU research is developing ways to accelerate the process.