SPECCC

Specialised Cell Carrier Components

Coordinatore	CELLON SA    more  Organization address address: RUE HEIERCHEN 16 ZAE ROBERT STEICHEN city: BASCHARAGE postcode: 4940 contact info Titolo: Mr. Nome: Richard Cognome: Fry Email: send email Telefono: 3522633731 Fax: 352311052
Nazionalità Coordinatore	Luxembourg [LU]
Totale costo	1˙425˙790 €
EC contributo	1˙065˙574 €
Programma	FP7-SME Specific Programme "Capacities": Research for the benefit of SMEs
Code Call	FP7-SME-2012
Funding Scheme	BSG-SME
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-11-01   -   2014-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	CELLON SA  Organization address address: RUE HEIERCHEN 16 ZAE ROBERT STEICHEN city: BASCHARAGE postcode: 4940 contact info Titolo: Mr. Nome: Richard Cognome: Fry Email: send email Telefono: 3522633731 Fax: 352311052	LU (BASCHARAGE)	coordinator	416˙470.00
2	DTL BIOTECHNOLOGY LIMITED  Organization address address: CLARA ROAD CARRAIG CLUAIN 107 city: TULLAMORE contact info Titolo: Mr. Nome: Denis Cognome: Looby Email: send email Telefono: 353579000000	IE (TULLAMORE)	participant	390˙396.00
3	SILICONE-ALTIMEX LIMITED  Organization address address: PASTURE ROAD STAPLEFORD 49 city: NOTTINGHAM postcode: NG9 8HR contact info Titolo: Mr. Nome: Christopher Cognome: Barker Email: send email Telefono: 441159000000 Fax: 441159000000	UK (NOTTINGHAM)	participant	244˙308.00
4	INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLOGICA  Organization address address: Av. da Republica, Quinta do Marques S/N city: OEIRAS postcode: 2781901 contact info Titolo: Prof. Nome: Paula Cognome: Alves Email: send email Telefono: 351214000000	PT (OEIRAS)	participant	5˙200.00
5	UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN  Organization address address: BELFIELD city: DUBLIN postcode: 4 contact info Titolo: Dr. Nome: Donal Cognome: Doolan Email: send email Telefono: 35317161656	IE (DUBLIN)	participant	5˙200.00
6	InSCREENeX GmbH  Organization address address: INHOFFENSTRASSE 7 city: BRAUNSCHWEIG postcode: 38124 contact info Titolo: Dr. Nome: Tobias Cognome: May Email: send email Telefono: +49 531 61815080 Fax: +49 531 61815002	DE (BRAUNSCHWEIG)	participant	4˙000.00

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 Obiettivo del progetto (Objective)

'Silicone if combined with appropriate cell adherent regulatory compounds offers significant advantages as a micro cell carrier, effectively a 3D microtissue device. These benefits include a capacity for defined and controlled porosity, inner chamber size, density, flexible shape, friction resistant, and low toxicity. The lead SME partner holds the IP for a silicone cell carrier device, which met some of these criteria, but exhibited poor cell adherence and requires re-engineering and exploitation of current cell molecular knowledge. There is a strong commercial need for such fully functional 3D cell culture technologies (microtissue) that can be manufactured into robust and reproducible in vitro test systems for toxicity testing, drug testing, cosmetic testing as part of a projected €1.6b cell based test market. This device may also eventually function as an active component in bioartificial livers (BAL) and other organ devices. The market growth is driven by needs in drug screening for drug discovery and toxicity screening. There are also a number of current EU directives that is driving the demand for reliable in vitro 3D cell based test system to replace in vivo test systems. The bio artificial liver market is also expected to grow rapidly due to high rates of liver disease worldwide. This project will develop novel develop new 3D cell culture technology based on proprietary cell immortalisation and the owned cell immobilisation technology. The objectives will be to deliver: 1) Immortalised hepatocyte and endothelial cells that retain characteristics of primary liver cells in 3D co-culture. 2) A porous silicone carrier for 3D culture 3) A scaleable bioreactor systems for 3D culture The following products will be developed using this technology, 1) 3D liver cell multiwell plate assay system, 2) 3D liver microbioreactor system and 3) a bioreactor component for a BAL device. Minimal SME economic return is predicted at €12m within 3 years of project completion'

Introduzione (Teaser)

Researchers are developing 3D cell-culture technology for growing bio-artificial livers, as well as artificial liver systems to test drug toxicity.

Descrizione progetto (Article)

Silicone is a good potential scaffold for growing artificial organs and 3D microtissue devices. However, further work is required to improve cell adherence and optimise various 3D microtissue systems for commercial application.

The 'Specialised cell carrier components' (http://www.biospeccc.com (SPECCC)) project builds on previous work on this system carried out at one of the project's industrial partner companies. The project is now developing several different systems using liver cells and a silicone framework.Researchers investigated the growth of liver cells in the first-generation silicone carriers. This initial work also looked into growing liver cells in carriers in bioreactors.

Project members have also made progress towards developing the liver cell lines required for the biological aspect of these systems. They have immortalised hepatocytes (liver cells) and liver endothelial cells from humans for this purpose.

SPECCC has also worked on the physical properties of the porous silicone carrier, such as pore volume, mechanical stability and cell surface properties. Researchers have tested plasma and collagen treatments of the silicone surface to help liver cells stick.The project will use this research to build prototype second-generation silicone carriers. These will have applications in drug and toxicity testing.