FLUARRAY

Development and validation of a microarray based automated diagnostic system for the detection of influenza virus types and subtypes at point-of-care

 Coordinatore UNIVERSITA DEGLI STUDI DI PERUGIA 

 Organization address address: PIAZZA DELL' UNIVERSITA 1
city: PERUGIA
postcode: 6123

contact info
Titolo: Prof.
Nome: Ennio
Cognome: Becchetti
Email: send email
Telefono: +39 07 55858222
Fax: +39 07 55858415

 Nazionalità Coordinatore Italy [IT]
 Totale costo 3˙642˙702 €
 EC contributo 2˙775˙993 €
 Programma FP7-HEALTH
Specific Programme "Cooperation": Health
 Code Call FP7-HEALTH-2007-A
 Funding Scheme CP-TP
 Anno di inizio 2008
 Periodo (anno-mese-giorno) 2008-01-01   -   2011-06-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    UNIVERSITA DEGLI STUDI DI PERUGIA

 Organization address address: PIAZZA DELL' UNIVERSITA 1
city: PERUGIA
postcode: 6123

contact info
Titolo: Prof.
Nome: Ennio
Cognome: Becchetti
Email: send email
Telefono: +39 07 55858222
Fax: +39 07 55858415

IT (PERUGIA) coordinator 0.00
2    HEALTH PROTECTION AGENCY HPA

 Organization address address: Central Office - 7th Floor, Holborn Gate - High Holborn 330
city: LONDON
postcode: WC1V 7PP

contact info
Titolo: Mr.
Nome: David
Cognome: Conway
Email: send email
Telefono: +44 208 3277028
Fax: +44 20 82051630

UK (LONDON) participant 0.00
3    ISTITUTO SUPERIORE DI SANITA

 Organization address address: Viale Regina Elena 299
city: ROMA
postcode: 161

contact info
Titolo: Dr.
Nome: Rosa Maria
Cognome: Martoccia
Email: send email
Telefono: +39 06 49902688
Fax: +39 06 49903007

IT (ROMA) participant 0.00
4    MICROTEST MATRICES LTD

 Organization address address: 14 Prices Gardens
city: LONDON
postcode: SW7 1NA

contact info
Titolo: Ms.
Nome: Clelia
Cognome: Supparo
Email: send email
Telefono: 442076000000
Fax: 442076000000

UK (LONDON) participant 0.00
5    PX'Therapeutics

 Organization address address: "15, rue des Martyrs"
city: GRENOBLE
postcode: 38027

contact info
Titolo: Dr.
Nome: Tristan
Cognome: Rousselle
Email: send email
Telefono: +33 438 023650
Fax: +33 476 961038

FR (GRENOBLE) participant 0.00
6    SEAC

 Organization address address: Via de Prato 74
city: CALENZANO- FIRENZE
postcode: 50041

contact info
Titolo: Dr.
Nome: Angelo
Cognome: Manzoni
Email: send email
Telefono: +39 055 8877469
Fax: +39 055 8877771

IT (CALENZANO- FIRENZE) participant 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

caused    diagnosis    viruses    components    simultaneously    influenza    assay    assays    affordable    distinct    sensitivity    simple    specimens    na    reading    multiplex    care    optical    subtypes    human    antibodies    performance    fluarray    automated    surveillance    types    sophisticated    reagents    infection    lateral    microarray    hundreds    capability    proteins    detection    perform    treatment    virus    device    distinguish    instrument    module    animal    capacity    immunoassays    immunoassay    tracer    flow    point    diagnostic    rapid    innovative   

 Obiettivo del progetto (Objective)

'Influenza is an extremely contagious infection that is caused by distinct virus types and subtypes. Early diagnosis is crucial for disease treatment and control as it reduces the inappropriate use of antibiotics and provides the indication for antiviral therapy. Rapid diagnosis is also a key component of surveillance activity. This requires the ability to detect and accurately diagnose infection at or close to the source/outbreak with minimum delay, a tactic consistent with the global experience during the SARS epidemic in 2003. This experience underlines the need for specific, sensitive point-of-care testing capable of discriminating between influenza subtypes. None of the available influenza diagnostic assays combines a point-of-care format with the multiplex capability to identify a large repertoire of human and animal viruses. This project exploits the knowledge and the expertise of the partners to convert microarray assays, that have a powerful multiplex capability but are laborious, complex and expensive to perform, into a simple, robust and affordable automated point-of-care system for the diagnosis of influenza. The system will utilize three components: 1) a microarray immunoassay that distinguishes influenza A and B virus as well as A subtypes; 2) an innovative self-contained disposable lateral-flow device that allows the addition of specimens and reagents in a temporally-controlled manner; 3) a robust automated processing reading instrument of novel conception that employs a low cost, high performance optical module. This project will provide small laboratories, health offices, veterinary clinics and outposts (airports) with the diagnostic capability of major research institutions and reference centres, thus providing better care for patients and most importantly, facilitating the implementation of surveillance activities and guiding response measures that are being built to face a possible influenza pandemic caused by a highly virulent virus.'

Introduzione (Teaser)

A timely diagnosis of influenza is the key to prompt treatment. A European consortium worked on developing an innovative diagnostic tool based on microarrays.

Descrizione progetto (Article)

Over the past years, a number of influenza diagnostic tools have been developed for point-of-care applications. They are based on dipstick lateral flow technology that utilises robust, affordable and easy to perform assays. However, rapid tests have limitations, such as lack of sensitivity, an inability to distinguish among different strains of influenza viruses and little multiplex capacity.

New test formats and technologies are required for determining numerous parameters simultaneously. In this context, the EU-funded http://www.fluarray.eu/ (FLUARRAY) (Development and validation of a microarray based automated diagnostic system for the detection of influenza virus types and subtypes at point-of-care) project proposed to develop a microarray-based system for point-of-care influenza diagnosis. This technology would dramatically increase assay throughput while reducing cost.

Microarray assays are currently processed manually and require equipment not suitable for the clinical laboratory. FLUARRAY converted the sophisticated microarray technology into a simple and robust diagnostic system. This system can simultaneously process hundreds of assays on a surface area the size of a fingernail. It will be used to distinguish between haemagglutinin (HA) and neuraminidase (NA) variants from major human and animal viruses in biological specimens.

The system essentially comprises a miniature immunoassay utilising a microarray of capture antibodies directed against the two influenza virus proteins, HA and NA. The array captures recombinant tracer proteins which are then sandwiched by a secondary fluorescently-labelled antibody. In the presence of influenza virus in the specimen, the tracer proteins are displaced by the viral antigens, causing a reduction in the recorded fluorescence signal.

The consortium has produced all the molecular components of the assay including the monoclonal antibodies and assessed its detection sensitivity. Scientists have validated the performance of the system but the desired sensitivity is yet to be achieved.

Considerable efforts were dedicated towards the production of a prototype automated microarray reading instrument that can add samples and reagents to the assay device. The instrument contains an optical module to read microarray immunoassays with a sensitivity and dynamic range similar to that of the most sophisticated microarray scanners.

FLUARRAYs portable point-of-care diagnostic system will perform hundreds of distinct parallel immunoassays offering high discriminatory capacity in the diagnosis of influenza.

Altri progetti dello stesso programma (FP7-HEALTH)

TRANSLINK (2013)

Defining the role of xeno-directed and autoimmune events in patients receiving animal-derived bioprosthetic heart valves

Read More  

COPING (2010)

"Children of Prisoners, Interventions & Mitigations to Strengthen Mental Health"

Read More  

CONCORD (2009)

CONtrol of COmmunity-acquired MRSA: Rationale and Development of counteractions

Read More