RECBREED

Recombination: an old and new tool for plant breeding

 Coordinatore Karlsruher Institut fuer Technologie 

 Organization address address: Kaiserstrasse 12
city: Karlsruhe
postcode: 76131

contact info
Titolo: Dr.
Nome: Holger
Cognome: Puchta
Email: send email
Telefono: 497216000000
Fax: 497216000000

 Nazionalità Coordinatore Germany [DE]
 Sito del progetto http://recbreed.eu/
 Totale costo 4˙174˙447 €
 EC contributo 2˙997˙147 €
 Programma FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology
 Code Call FP7-KBBE-2008-2B
 Funding Scheme CP-FP
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-03-01   -   2013-02-28

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    Karlsruher Institut fuer Technologie

 Organization address address: Kaiserstrasse 12
city: Karlsruhe
postcode: 76131

contact info
Titolo: Dr.
Nome: Holger
Cognome: Puchta
Email: send email
Telefono: 497216000000
Fax: 497216000000

DE (Karlsruhe) coordinator 451˙460.00
2    WEIZMANN INSTITUTE OF SCIENCE

 Organization address address: HERZL STREET 234
city: REHOVOT
postcode: 7610001

contact info
Titolo: Ms.
Nome: Talia
Cognome: Tzahor
Email: send email
Telefono: +972 8 934 4026
Fax: +972 8934 4165

IL (REHOVOT) participant 405˙220.00
3    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

 Organization address address: Rue Michel -Ange 3
city: PARIS
postcode: 75794

contact info
Titolo: Dr.
Nome: Sandrine
Cognome: Magnetto
Email: send email
Telefono: +334 72 44 56 41
Fax: 33478890851

FR (PARIS) participant 404˙169.00
4    UNIVERSITEIT LEIDEN

 Organization address address: RAPENBURG 70
city: LEIDEN
postcode: 2300 RA

contact info
Titolo: Mr.
Nome: Ton
Cognome: Brouwer
Email: send email
Telefono: 31715273149
Fax: 31715275269

NL (LEIDEN) participant 400˙302.00
5    UNIVERSITE DE GENEVE

 Organization address address: Rue du General Dufour 24
city: GENEVE
postcode: 1211

contact info
Titolo: Prof.
Nome: Jerzy
Cognome: Paszkowski
Email: send email
Telefono: +41 22 379 30 21
Fax: +41 22 379 31 07

CH (GENEVE) participant 396˙708.00
6    KEYGENE NV

 Organization address address: AGRO BUSINESS PARK 90 90
city: WAGENINGEN
postcode: 6700AE

contact info
Titolo: Dr.
Nome: Michiel
Cognome: De Both
Email: send email
Telefono: +31 317 466 866
Fax: +31 317 424 939

NL (WAGENINGEN) participant 389˙308.00
7    BIOGEMMA

 Organization address address: Rue E. Colonne 1
city: PARIS
postcode: 75001

contact info
Titolo: Dr.
Nome: Catherine
Cognome: Guichard
Email: send email
Telefono: 33155349403
Fax: 33155349401

FR (PARIS) participant 369˙500.00
8    OSM-DAN LTD.

 Organization address address: Pekeris Street 4
city: REHOVOT
postcode: 76702

contact info
Titolo: Dr.
Nome: Pnina
Cognome: Dan
Email: send email
Telefono: +972 8 9460012
Fax: +972 8 9361486

IL (REHOVOT) participant 180˙480.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

cells    efficient    homologous    yield    maize    plant    hr    recbreed    induction    zinc    food    steps    artificial    methylation    dna    gt    precise    rely    repair    arabidopsis    double    plants    homologue    crops    finger    induced    resistance    break    successfully    resolution    dsb    invasion    desirable    somatic    affecting    agricultural    traits    genes    breeding    recombination    gene    nucleases    genomes    performed    stage    proteins    meiotic    techniques    strategies    tomato    rad    engineering    wp   

 Obiettivo del progetto (Objective)

'Successful and efficient plant breeding depends on rapid recombination of advantageous traits to form new crop varieties. In recent years new breeding techniques have been introduced which rely on transgenic alteration of somatic cells and regeneration into plants with novel properties. The precision and effectiveness of both strategies rely upon homologous recombination (HR). The objective of this proposal is to provide plant breeders with new tools allowing better control over HR in both somatic and meiotic cells. The expected outcomes of the proposed research are efficient gene targeting (GT) technologies for precise engineering of plant genomes and control of rates of meiotic recombination between homologous or homeologous chromosomes in classical breeding. The major components of the HR machinery are common to somatic and meiotic cells, enabling us to address both processes in a synergistic way. HR can be divided into different steps: initiation by formation of a DNA double-strand break (DSB); recognition and invasion of an homologous DNA sequence; resolution of recombination structures. Each stage contains a bottleneck for both GT and meiotic HR that we will address. Work package 1 (WP1) aims at enhancing HR through targeted DSB induction. DSBs will be induced by Zinc-finger nucleases that can be custom-designed for target sequences anywhere in the genome. In WP2, we will test the influence of HR factors affecting homologue invasion and heteroduplex formation, such as RAD51 and its paralogues, the RAD52 homologue, genes that affect cytosine methylation in DNA, and mismatch repair. In WP3 we will concentrate on proteins involved in resolution and crossing-over. WP4 will test combinations of those approaches found in the first three WPs to build optimal strategies for application. Most experiments will be performed in the model plant Arabidopsis and implemented into crops such as tomato and maize to guarantee quick applicability for breeding.'

Introduzione (Teaser)

Rising population numbers have raised the demand for food, clothing and other necessities. EU funding is helping to breed plants with desirable traits such as improved fungi resistance, drought resistance and high yield to meet these growing needs.

Descrizione progetto (Article)

The EU-funded project 'Recombination: An old and new tool for plant breeding' (http://recbreed.eu/ (RECBREED)) was initiated to realise the development of 'super' plants. The RECBREED consortium worked on optimising plant breeding by developing a better toolkit to produce plants with desirable traits and weed out undesirable traits.

They explored different homologous recombination (HR) techniques enables the exchange of genetic material for purposes of DNA repair, reproduction (also called meiosis) and artificial gene transfer between different species or strains. Techniques included gene targeting (GT), meiotic recombination and DNA double-stranded break induction (DSB) by artificial nucleases.

RECBREED achieved several milestones in the accurate engineering of plant genomes. Each stage in HR was studied in depth by characterising important genes and elucidating their function using selected crops such as tomato, maize and Arabidopsis. Suitable molecular markers were identified through bioinformatics analyses and used for testing and validation.

Scientists successfully performed DSB-induced mutagenesis and GT in Arabidopsis and tomato using zinc finger nucelases and transcription activator-like effector nucleases. No toxic or negative plant growth effects were observed with either of these methods. An innovative GT method called in planta was developed that separates the transformation and GT steps, and its efficacy was demonstrated in maize and Arabidopsis. Another key achievement was a 10-fold increase in precise GT rate achieved by enhancing GT frequencies through over-expression of the protein AtRAD52-1A.

Particular emphasis was placed on understanding and modulating factors affecting meiotic recombination. Key parameters such as Rad51 paralog proteins, DNA methylation states, chromatin structure and recombinases (Rad51 and Dmc1) were successfully identified.

Conventional agricultural techniques have been proven inadequate when it comes to meeting the rapidly growing demands for food and feed. Use of GT technology could prove to be viable for industrial production of superior high-yield, high-nutrition plants. This has important socioeconomic implications for the European agricultural sector with a current market share of about EUR 4 000 million in seed sales alone. Combining agriculture with biotechnology will only serve to further strengthen the EU position in this arena.

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