ELECTROEXTRACTION

Protein electroextraction coupled to direct sorption – a new route for primary recovery of intracellular bioproducts from industrial yeast

 Coordinatore JACOBS UNIVERSITY BREMEN GGMBH 

 Organization address address: Campus Ring 1
city: BREMEN
postcode: 28759

contact info
Titolo: Mr.
Nome: Ronald
Cognome: Kieschnick
Email: send email
Telefono: -2004887
Fax: -200494887

 Nazionalità Coordinatore Germany [DE]
 Sito del progetto http://www.electroextraction.org
 Totale costo 1˙316˙800 €
 EC contributo 946˙440 €
 Programma FP7-SME
Specific Programme "Capacities": Research for the benefit of SMEs
 Code Call FP7-SME-2007-1
 Funding Scheme BSG-SME
 Anno di inizio 2008
 Periodo (anno-mese-giorno) 2008-12-01   -   2011-05-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    JACOBS UNIVERSITY BREMEN GGMBH

 Organization address address: Campus Ring 1
city: BREMEN
postcode: 28759

contact info
Titolo: Mr.
Nome: Ronald
Cognome: Kieschnick
Email: send email
Telefono: -2004887
Fax: -200494887

DE (BREMEN) coordinator 0.00
2    ( Research Organization and Manufacture of Bioproducts OOD)

 Organization address address: "Aboba St. 1, APP 5 FL 3"
city: SOFIA
postcode: 1606

contact info
Titolo: Dr.
Nome: Valentin
Cognome: Savov
Email: send email
Telefono: +3592950 14 40
Fax: +3592950 14 41

BG (SOFIA) participant 0.00
3    42 life sciences KG

 Organization address address: Fischkai 1
city: Bremerhaven
postcode: 27572

contact info
Titolo: Dr.
Nome: Piere
Cognome: Rogalla
Email: send email
Telefono: +49 471 4832500
Fax: +49 471 4832308

DE (Bremerhaven) participant 0.00
4    BETATECH

 Organization address address: RUE D APOLLO Z.A. de Montredon 15
city: L UNION
postcode: 31240

contact info
Titolo: Mr.
Nome: Jean-Marc
Cognome: Anizan
Email: send email
Telefono: +335 34 30 40 00
Fax: +33 5 34 30 10 47

FR (L UNION) participant 0.00
5    BIOMEDAL SL

 Organization address address: CUBA 1-1B
city: SEVILLA
postcode: 41100

contact info
Titolo: Dr.
Nome: Angel
Cognome: Cebolla Ramírez
Email: send email
Telefono: 34954081276
Fax: 34954081279

ES (SEVILLA) participant 0.00
6    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

 Organization address address: Rue Michel -Ange 3
city: PARIS
postcode: 75794

contact info
Titolo: Dr.
Nome: Justin
Cognome: Teissie
Email: send email
Telefono: +335 61 17 5812
Fax: +335 61 17 5994

FR (PARIS) participant 0.00
7    INFOCONSULT GESELLSCHAFT FUR INFORMATIONSTECHNIK MBH

 Organization address address: ANNE CONWAY STRASSE 4
city: BREMEN
postcode: 28359

contact info
Titolo: Dr.
Nome: Peter
Cognome: Knackfuß
Email: send email
Telefono: +40 421 3306480
Fax: +49 421 3306489

DE (BREMEN) participant 0.00
8    ORGANOBALANCE GmbH

 Organization address address: Gustav-Meyer-Allee 25
city: BERLIN
postcode: 13355

contact info
Titolo: Prof.
Nome: Christine
Cognome: Lang
Email: send email
Telefono: +49 30 46307-200
Fax: +49 30 46307-210

DE (BERLIN) participant 0.00
9    PHYTOLUTIONS GMBH

 Organization address address: CAMPUS RING 1
city: BREMEN
postcode: 28759

contact info
Nome: Brigitte
Cognome: Schweitzer
Email: send email
Telefono: +49 421 566272 13
Fax: +49 421 566272 27

DE (BREMEN) participant 0.00
10    SOFIISKI UNIVERSITET SVETI KLIMENT OHRIDSKI

 Organization address address: Tsar Osvoboditel Blvd. 15
city: SOFIA
postcode: 1504

contact info
Titolo: Prof.
Nome: Valentina
Cognome: Ganeva
Email: send email
Telefono: 35928654686
Fax: +3592 8656641

BG (SOFIA) participant 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

interfering    recovering    treated    suitable    real    sequestration    ligand    yields    electrically    selectivity    continuous    powerful    feedstock    food    commercial    damage    envelope    electroextraction    debris    substances    acids    microbial    performed    coupling    direct    selective    soluble    protease    intracellular    cytoplasmic    mode    capture    yeasts    chemical    treatment    electric    yeast    sites    limited    solid    bioproducts    compatibility    purification    pharmaceutical    onto    release    experiments    adsorbents    protein    organelles    total    efficient    recovery    algae    industries    proteins    hosts    laboratory    fermentations    species    intensity    pulses    beaded    sorption    disintegration    cell    adsorbent    nucleic    led    bacteria    technique    mass   

 Obiettivo del progetto (Objective)

'This project proposes a new -highly efficient- method for intracellular protein recovery form yeast – electroextraction. Moreover, a novel bio processing strategy will be developed based on its combination with subsequent direct product capture onto a suitable solid phase. Selective recovery of the yeast intracellular soluble proteome will be attempted by permeabilization of cell envelope with high intensity electric field pulses. This treatment as already shown in laboratory experiments leads to a selective release of soluble cytoplasmic proteins, without cell disintegration and with high product yields. The electropermeabilization will be performed in continuous mode under conditions allowing greater selectivity for the targeted species, and limited protease release to avoid product damage. The method will be tested employing several types of commercially relevant yeasts. Sorption is a proven method for efficient product recovery from fermentation liquors or disrupted biomass. The compatibility of the electrically treated feedstock with commercial beaded adsorbents for direct product capture will be assessed, under real process conditions. Operational windows will be defined to allow for product sorption in stirred tank or fluidized bed contactors. Mass transfer properties of the whole system will be explored. Better utilization of total adsorbent ligand sites is expected since less potential interfering substances (nucleic acids, organelles, cell debris) will be liberated. Therefore, a powerful technology for intracellular protein recovery and purification can be envisioned by coupling electroextraction and immediate soluble product sequestration onto a suitable solid phase. ‘Electroextraction’ will provide a route for more facile, efficient, and economical processing of intracellular bioproducts from yeast fermentations that are valuable for the chemical, food, and pharmaceutical industries.'

Introduzione (Teaser)

Mass production of bioproducts has been held back by the high cost of their recovering and purification. But now things are about to change thanks to an EU-funded initiative that has developed a new, simple and inexpensive processing system for intracellular products produced from industrial yeast.

Descrizione progetto (Article)

The ELECTROEXTRACTION project developed a technique for recovering intracellular-derived products that were biosynthesised in microbial hosts such as yeasts, algae and bacteria. The electroextraction process was an easy, selective and cost-effective method for releasing intracellular products with minimal damage. It was based on protein liberation by rendering the cell envelope more permeable with high-intensity electric field pulses.

Laboratory experiments showed that this treatment led to the selective release of soluble cytoplasmic proteins without cell disintegration and with high product yields. The electro-permeabilisation was performed in continuous mode under conditions that allowed greater selectivity for the targeted species and limited protease release to avoid product damage.

This approach led to an increase in final product clarity as a result of gentle treatment and selective isolation. Increased process yield was also achieved due to a reduction in the number of steps required for purification and recovery (commonly referred to as downstream processing). The technique worked with yeast cells acting as hosts to natural or recombinant proteins and enzymes. However, electroextraction was non-applicable to other microbial hosts like bacteria, which ruptured, or algae, which possessed rigid cell walls.

Researchers also assessed the compatibility of the electrically treated feedstock with commercial beaded adsorbents for direct product capture under real process conditions. Scientists expected better utilisation of total adsorbent ligand sites as less potentially interfering substances such as nucleic acids, organelles and cell debris were released.

Project partners believed a powerful technology for intracellular protein recovery and purification could be realised by coupling electroextraction and immediate soluble product sequestration onto a suitable solid phase.

Electroextraction provided a straightforward, efficient and cost-effective way of recovering intracellular bioproducts from yeast fermentations that can be used in the chemical, food and pharmaceutical industries.

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