DISENTANGLE

Untangling the Bacterial Chromosome: Condensin's Role in Sister Chromosome Separation and its Mechanisms

 Coordinatore MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. 

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 Nazionalità Coordinatore Germany [DE]
 Totale costo 1˙376˙734 €
 EC contributo 1˙376˙734 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2010-StG_20091118
 Funding Scheme ERC-SG
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-11-01   -   2015-10-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

 Organization address address: Hofgartenstrasse 8
city: MUENCHEN
postcode: 80539

contact info
Titolo: Dr.
Nome: Anne Katrin
Cognome: Werenskiold
Email: send email
Telefono: 498986000000
Fax: 498986000000

DE (MUENCHEN) hostInstitution 1˙376˙734.00
2    MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

 Organization address address: Hofgartenstrasse 8
city: MUENCHEN
postcode: 80539

contact info
Titolo: Dr.
Nome: Stephan
Cognome: Gruber
Email: send email
Telefono: +49898578 3440
Fax: +49898578 3430

DE (MUENCHEN) hostInstitution 1˙376˙734.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

protein    sister    dna    bacteria    molecules    helix    ring    chromosomes    decatenation    topoisomerases    hypothesis    unlinking    chromosome    double    chromatids    complexes    segregation    smc    condensin    separation   

 Obiettivo del progetto (Objective)

'A prerequisite for chromosome segregation in all living organisms is the topological unlinking of sister DNA molecules, called DNA decatenation. Decatenation is performed by DNA topoisomerases that work by transiently breaking strand(s) in one DNA double helix and passing another double helix through the temporarily created gate. How DNA topoisomerases manage to recognize linkages between sister DNA molecules and how they promote decatenation of sister chromatids (but not catenation) is still largely unknown. The driving hypothesis of this project is that condensin promotes chromosome decatenation by guiding the unlinking activity of DNA topoisomerases. Condensin is a member of the family of SMC (Structural Maintenance of Chromosomes) protein complexes that is conserved from bacteria to humans. It forms large, ring-like structures that bind to and organize chromosomes. Efficient separation of sister chromosomes in the bacterium B. subtilis depends on the condensin complex. However, so far the precise role of condensin in chromosome segregation and its mechanisms are unclear. To test our hypothesis, we will establish a minichromosome in bacteria that segregates in a condensin-dependent manner and measure its decatenation in vivo in the presence and absence of condensin. We will investigate the mechanism by which condensin organizes DNA within the (mini-)chromosome using techniques like chromosome conformation capture (3C) and electron microscopy. Finally, we will attempt to reconstitute for the first time the entrapment of DNA double helices by ring-like SMC protein complexes using purified components. Our results will be pivotal for understanding the action of SMC proteins in general with important implications in the separation and segregation of chromosomes in bacteria, the shaping of mitotic chromosomes and the resolution of sister chromatids during mitosis in eukaryotes.'

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