SIMBA

Single-Molecule BioAssays at Elevated Concentrations

Coordinatore	TECHNISCHE UNIVERSITAT BRAUNSCHWEIG    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	Germany [DE]
Totale costo	1˙456˙374 €
EC contributo	1˙456˙374 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2010-StG_20091118
Funding Scheme	ERC-SG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-11-01   -   2016-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	TECHNISCHE UNIVERSITAT BRAUNSCHWEIG  Organization address address: POCKELSSTRASSE 14 city: BRAUNSCHWEIG postcode: 38106 contact info Titolo: Ms. Nome: Kirsten-Ilona Cognome: Talk Email: send email Telefono: 495314000000 Fax: 495314000000	DE (BRAUNSCHWEIG)	hostInstitution	1˙456˙374.00
2	TECHNISCHE UNIVERSITAT BRAUNSCHWEIG  Organization address address: POCKELSSTRASSE 14 city: BRAUNSCHWEIG postcode: 38106 contact info Titolo: Prof. Nome: Philip Karl-Josef Cognome: Tinnefeld Email: send email Telefono: 495314000000 Fax: +49 531 391 5832	DE (BRAUNSCHWEIG)	hostInstitution	1˙456˙374.00

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 Obiettivo del progetto (Objective)

'In order to advance single-molecule fluorescence spectroscopy to the next level, handling and analysis of single molecules has to become broadly available. A further quantum leap is required to proceed to commercially successful applications such as drug screening and medical diagnostics. In this project, I suggest a strategy to overcome the fundamental gap between the nanomolar concentration regime of current optical single-molecule spectroscopy and the nano- to millimolar dissociation constants of typical biomolecular interactions. I will use nano-apertures, which confine the detection to sub-attoliter volumes and allow single-molecule studies at elevated concentrations. To overcome unspecific binding and deteriorated fluorescence signals in the nano-apertures, I will use tailor-made DNA nanostructures produced by DNA origami. These nanostructures will match the nano-apertures like a plug in a socket. Inserting molecules at programmed positions in the nanostructures will open up a new realm of applications by the ability to immobilize exactly one molecule per nano-aperture and by obtaining comparable signals from every nano-aperture. I will spectroscopically characterize the nano-apertures creating a fluorescence map of their inside. I will exemplarily use the new abilities for previously impossible applications such as several folds improvement of single-molecule DNA sequencing, direct single-molecule RNA sequencing by reverse transcriptase for cancer screening, for paralleled drug screening of HIV protease inhibitors and for studying the chemomechanical coupling of single helicases. In summary, I envision a broadly applicable platform that has the potential to become a golden standard by enabling both ground breaking fundamental research and commercial applications.'