SIMBA

Single-Molecule BioAssays at Elevated Concentrations

 Coordinatore TECHNISCHE UNIVERSITAT BRAUNSCHWEIG 

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 Nazionalità Coordinatore Germany [DE]
 Totale costo 1˙456˙374 €
 EC contributo 1˙456˙374 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2010-StG_20091118
 Funding Scheme ERC-SG
 Anno di inizio 2010
 Periodo (anno-mese-giorno) 2010-11-01   -   2016-10-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAT BRAUNSCHWEIG

 Organization address address: POCKELSSTRASSE 14
city: BRAUNSCHWEIG
postcode: 38106

contact info
Titolo: Ms.
Nome: Kirsten-Ilona
Cognome: Talk
Email: send email
Telefono: 495314000000
Fax: 495314000000

DE (BRAUNSCHWEIG) hostInstitution 1˙456˙374.00
2    TECHNISCHE UNIVERSITAT BRAUNSCHWEIG

 Organization address address: POCKELSSTRASSE 14
city: BRAUNSCHWEIG
postcode: 38106

contact info
Titolo: Prof.
Nome: Philip Karl-Josef
Cognome: Tinnefeld
Email: send email
Telefono: 495314000000
Fax: +49 531 391 5832

DE (BRAUNSCHWEIG) hostInstitution 1˙456˙374.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

overcome    sequencing    aperture    nano    molecules    fluorescence    single    broadly    dna    become    nanostructures    spectroscopy    screening    signals    apertures    molecule    fundamental    drug   

 Obiettivo del progetto (Objective)

'In order to advance single-molecule fluorescence spectroscopy to the next level, handling and analysis of single molecules has to become broadly available. A further quantum leap is required to proceed to commercially successful applications such as drug screening and medical diagnostics. In this project, I suggest a strategy to overcome the fundamental gap between the nanomolar concentration regime of current optical single-molecule spectroscopy and the nano- to millimolar dissociation constants of typical biomolecular interactions. I will use nano-apertures, which confine the detection to sub-attoliter volumes and allow single-molecule studies at elevated concentrations. To overcome unspecific binding and deteriorated fluorescence signals in the nano-apertures, I will use tailor-made DNA nanostructures produced by DNA origami. These nanostructures will match the nano-apertures like a plug in a socket. Inserting molecules at programmed positions in the nanostructures will open up a new realm of applications by the ability to immobilize exactly one molecule per nano-aperture and by obtaining comparable signals from every nano-aperture. I will spectroscopically characterize the nano-apertures creating a fluorescence map of their inside. I will exemplarily use the new abilities for previously impossible applications such as several folds improvement of single-molecule DNA sequencing, direct single-molecule RNA sequencing by reverse transcriptase for cancer screening, for paralleled drug screening of HIV protease inhibitors and for studying the chemomechanical coupling of single helicases. In summary, I envision a broadly applicable platform that has the potential to become a golden standard by enabling both ground breaking fundamental research and commercial applications.'

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