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SMPFv2.0 SIGNED

Next generation single molecule protein fluorescence

Total Cost €

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EC-Contrib. €

0

Partnership

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 SMPFv2.0 project word cloud

Explore the words cloud of the SMPFv2.0 project. It provides you a very rough idea of what is the project "SMPFv2.0" about.

power    superresolution    multiparameter    probing    scales    unleash    purified    fundamental    live    time    laborious    modification    workflows    microfluidic    generalised    disordered    structure    complexity    single    cell    dramatically    platform    distances    blur    vital    super    size    polymeric    science    hybrid    techniques    ultrafast    synthetic    complementary    machineries    readout    biotechnology    mechanisms    limit    spatial    molecule    function    exceeding    vitro    sampling    sciences    energy    protein    variety    automated    powerful    observation    data    limited    acquisition    intrinsically    fret    transfer    physicochemical    engage    unified    biochemical    populate    dynamics    preparation    readouts    binding    workflow    fluorescence    boundaries    analytical    idps    biology    mechanistic    fluorescent    cellular    resonance    semi    vivo    engineering    complexes    proteins    microscopy    specimen    tool    nano    resolution    popular    molecular    throughput    drug    statistical    sensitivity    technologies    discovery    lab    disease    chip    true    multifunctionality    biomedical   

Project "SMPFv2.0" data sheet

The following table provides information about the project.

Coordinator
JOHANNES GUTENBERG-UNIVERSITAT MAINZ 

Organization address
address: SAARSTRASSE 21
city: MAINZ
postcode: 55122
website: www.uni-mainz.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙996˙990 €
 EC max contribution 1˙996˙990 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    JOHANNES GUTENBERG-UNIVERSITAT MAINZ DE (MAINZ) coordinator 570˙013.00
2    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) participant 1˙426˙977.00

Map

 Project objective

Fluorescence techniques provide powerful means to study single protein machineries. Single molecule observation of Fluorescence Resonance Energy Transfer (FRET) has become an important tool for probing structure, distances, dynamics, physicochemical properties and size of purified (in vitro) protein complexes. Super-resolution techniques provide complementary spatial information about proteins in live (in vivo) specimen. Although these techniques have become very popular because of their high sensitivity, their throughput is limited by large statistical sampling requirements, complex sample preparation procedures and/or laborious data acquisition workflows. Here, I propose to design a unified single molecule approach by engineering ultrafast, semi-synthetic protein modification techniques and fluorescent readouts into nano/microfluidic hybrid devices. The development of an automated all-in-one Lab-on-a-Chip platform will dramatically improve throughput of single molecule fluorescence techniques. The technology will provide a multiparameter readout of molecular protein mechanisms across time, resolution and complexity scales in a generalised workflow. To demonstrate the power of the new platform, I will apply it to a mechanistic study of the multifunctionality of intrinsically disordered proteins (IDPs), which are vital to cellular function and associated with many disease mechanisms. The polymeric properties of IDPs enable them to populate a variety of states and engage with various cellular binding partners, thus exceeding the sampling limit of existing single molecule technologies. The new method will blur the boundaries between in cell superresolution microscopy and biochemical single molecule studies and unleash the true power of single molecule protein science for a range of applications in analytical sciences, drug discovery, biotechnology, systems biology and fundamental biomedical research.

 Publications

year authors and title journal last update
List of publications.
2020 Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke
The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
published pages: , ISSN: 0021-9525, DOI: 10.1083/jcb.201907157
The Journal of Cell Biology 219/1 2020-04-14
2018 Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, Mikayel Aznauryan, Anders Barth, Victoria Birkedal, Mark E. Bowen, Hongtao Chen, Thorben Cordes, Tobias Eilert, Carel Fijen, Christian Gebhardt, Markus Götz, Giorgos Gouridis, Enrico Gratton, Taekjip Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lass
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
published pages: 669-676, ISSN: 1548-7091, DOI: 10.1038/s41592-018-0085-0
Nature Methods 15/9 2020-04-14
2019 Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke
Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes
published pages: eaaw2644, ISSN: 0036-8075, DOI: 10.1126/science.aaw2644
Science 363/6434 2020-04-14
2018 Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”
published pages: eaau8230, ISSN: 0036-8075, DOI: 10.1126/science.aau8230
Science 361/6405 2020-04-14
2019 Aritra Chowdhury, Sergey A. Kovalenko, Iker Valle Aramburu, Piau Siong Tan, Nikolaus P. Ernsting, Edward A. Lemke
Mechanism-Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes
published pages: 4720-4724, ISSN: 1433-7851, DOI: 10.1002/anie.201813354
Angewandte Chemie International Edition 58/14 2020-04-14
2018 Sofya Mikhaleva, Edward A. Lemke
Beyond the Transport Function of Import Receptors: What’s All the FUS about?
published pages: 549-553, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.04.002
Cell 173/3 2020-04-14
2016 Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V. Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A. Lemke
Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy
published pages: 16172-16176, ISSN: 1433-7851, DOI: 10.1002/anie.201608284
Angewandte Chemie International Edition 55/52 2020-04-14
2017 Iker Valle Aramburu, Edward A. Lemke
Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors
published pages: 34-41, ISSN: 1084-9521, DOI: 10.1016/j.semcdb.2017.06.026
Seminars in Cell & Developmental Biology 68 2020-04-14
2016 Edward A. Lemke
The Multiple Faces of Disordered Nucleoporins
published pages: 2011-2024, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2016.01.002
Journal of Molecular Biology 428/10 2020-04-14
2018 Piau Siong Tan, Iker Valle Aramburu, Davide Mercadante, Swati Tyagi, Aritra Chowdhury, Daniel Spitz, Sarah L. Shammas, Frauke Gräter, Edward A. Lemke
Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors
published pages: 3660-3671, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2018.03.022
Cell Reports 22/13 2020-04-14
2016 Niccolò Banterle, Edward A Lemke
Nanoscale devices for linkerless long-term single-molecule observation
published pages: 105-112, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2016.02.013
Current Opinion in Biotechnology 39 2020-04-14

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