Opendata, web and dolomites

BIZEB SIGNED

Bio-Imaging of Zoonotic and Emerging Bunyaviruses

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

Project "BIZEB" data sheet

The following table provides information about the project.

Coordinator
HELSINGIN YLIOPISTO 

Organization address
address: YLIOPISTONKATU 3
city: HELSINGIN YLIOPISTO
postcode: 14
website: www.helsinki.fi

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Finland [FI]
 Project website https://www.strubi.ox.ac.uk/research/juha-huiskonen
 Total cost 1˙998˙375 €
 EC max contribution 1˙998˙375 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2020-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HELSINGIN YLIOPISTO FI (HELSINGIN YLIOPISTO) coordinator 652˙741.00
2    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) participant 1˙345˙633.00

Map

 Project objective

We aim to understand host cell entry of enveloped viruses at molecular level. A crucial step in this process is when the viral membrane fuses with the cell membrane. Similarly to cell–cell fusion, this step is mediated by fusion proteins (classes I–III). Several medically important viruses, notably dengue and many bunyaviruses, harbour a class II fusion protein. Class II fusion protein structures have been solved in pre- and post-fusion conformation and in some cases different factors promoting fusion have been determined. However, questions about the most important steps of this key process remain unanswered. I will focus on the entry mechanism of bunyaviruses by using cutting-edge, high spatial and temporal resolution bio-imaging techniques. These viruses have been chosen as a model system to maximise the significance of the project: they form an emerging viral threat to humans and animals, no approved vaccines or antivirals exist for human use and they are less studied than other class II fusion protein systems. Cryo-electron microscopy and tomography will be used to solve high-resolution structures (up to ~3 Å) of viruses, in addition to virus–receptor and virus–membrane complexes. Advanced fluorescence microscopy techniques will be used to probe the dynamics of virus entry and fusion in vivo and in vitro. Deciphering key steps in virus entry is expected to contribute to rational vaccine and drug design. During this project I aim to establish a world-class laboratory in structural and cellular biology of emerging viruses. The project greatly benefits from our unique biosafety level 3 laboratory offering advanced bio-imaging techniques. Furthermore it will also pave way for similar projects on other infectious viruses. Finally the novel computational image processing methods developed in this project will be broadly applicable for the analysis of flexible biological structures, which often pose the most challenging yet interesting questions in structural biology.

 Publications

year authors and title journal last update
List of publications.
2019 Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S. Walter, Eduardo A. Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan M. Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, David I. Stuart, Elina Roine
The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-08728-7
Nature Communications 10/1 2019-09-02
2018 Max Renner, Aleksandra Flanagan, Wanwisa Dejnirattisai, Chunya Puttikhunt, Watchara Kasinrerk, Piyada Supasa, Wiyada Wongwiwat, Kriangkrai Chawansuntati, Thaneeya Duangchinda, Alison Cowper, Claire M. Midgley, Prida Malasit, Juha T. Huiskonen, Juthathip Mongkolsapaya, Gavin R. Screaton, Jonathan M. Grimes
Characterization of a potent and highly unusual minimally enhancing antibody directed against dengue virus
published pages: 1248-1256, ISSN: 1529-2908, DOI: 10.1038/s41590-018-0227-7
Nature Immunology 19/11 2019-09-02
2018 Yasunori Watanabe, Jayna Raghwani, Joel D. Allen, Gemma E. Seabright, Sai Li, Felipe Moser, Juha T. Huiskonen, Thomas Strecker, Thomas A. Bowden, Max Crispin
Structure of the Lassa virus glycan shield provides a model for immunological resistance
published pages: 7320-7325, ISSN: 0027-8424, DOI: 10.1073/pnas.1803990115
Proceedings of the National Academy of Sciences 115/28 2019-09-02
2019 Luigi De Colibus, Elina Roine, Thomas S. Walter, Serban L. Ilca, Xiangxi Wang, Nan Wang, Alan M. Roseman, Dennis Bamford, Juha T. Huiskonen, David I. Stuart
Assembly of complex viruses exemplified by a halophilic euryarchaeal virus
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09451-z
Nature Communications 10/1 2019-09-02
2019 Serban L. Ilca, Xiaoyu Sun, Kamel El Omari, Abhay Kotecha, Felix de Haas, Frank DiMaio, Jonathan M. Grimes, David I. Stuart, Minna M. Poranen, Juha T. Huiskonen
Multiple liquid crystalline geometries of highly compacted nucleic acid in a dsRNA virus
published pages: 252-256, ISSN: 0028-0836, DOI: 10.1038/s41586-019-1229-9
Nature 570/7760 2019-09-02
2019 César López-Camacho, Young Chan Kim, Joshua Blight, Marcos Lazaro Moreli, Eduardo Montoya-Diaz, Juha T Huiskonen, Beate Mareike Kümmerer, Arturo Reyes-Sandoval
Assessment of Immunogenicity and Neutralisation Efficacy of Viral-Vectored Vaccines Against Chikungunya Virus
published pages: 322, ISSN: 1999-4915, DOI: 10.3390/v11040322
Viruses 11/4 2019-09-02
2018 Elizabeth R. Allen, Stefanie A. Krumm, Jayna Raghwani, Steinar Halldorsson, Angela Elliott, Victoria A. Graham, Elina Koudriakova, Karl Harlos, Daniel Wright, George M. Warimwe, Benjamin Brennan, Juha T. Huiskonen, Stuart D. Dowall, Richard M. Elliott, Oliver G. Pybus, Dennis R. Burton, Roger Hewson, Katie J. Doores, Thomas A. Bowden
A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus
published pages: 3750-3758.e4, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2018.12.001
Cell Reports 25/13 2019-09-02
2015 Serban L. Ilca, Abhay Kotecha, Xiaoyu Sun, Minna M. Poranen, David I. Stuart, Juha T. Huiskonen
Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes
published pages: 8843, ISSN: 2041-1723, DOI: 10.1038/ncomms9843
Nature Communications 6 2019-09-02
2018 Steinar Halldorsson, Sai Li, Mengqiu Li, Karl Harlos, Thomas A. Bowden, Juha T. Huiskonen
Shielding and activation of a viral membrane fusion protein
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-02789-2
Nature Communications 9/1 2019-09-02
2017 Nayab Malik, Abhay Kotecha, Sarah Gold, Amin Asfor, Jingshan Ren, Juha T. Huiskonen, Tobias J. Tuthill, Elizabeth E. Fry, David I. Stuart
Structures of foot and mouth disease virus pentamers: Insight into capsid dissociation and unexpected pentamer reassociation
published pages: e1006607, ISSN: 1553-7374, DOI: 10.1371/journal.ppat.1006607
PLOS Pathogens 13/9 2019-09-02
2017 Ilona Rissanen, Robert Stass, Antra Zeltina, Sai Li, Jussi Hepojoki, Karl Harlos, Robert J. C. Gilbert, Juha T. Huiskonen, Thomas A. Bowden
Structural Transitions of the Conserved and Metastable Hantaviral Glycoprotein Envelope
published pages: e00378-17, ISSN: 0022-538X, DOI: 10.1128/JVI.00378-17
Journal of Virology 91/21 2019-09-02
2017 Mariana Grieben, Ashley C W Pike, Chitra A Shintre, Elisa Venturi, Sam El-Ajouz, Annamaria Tessitore, Leela Shrestha, Shubhashish Mukhopadhyay, Pravin Mahajan, Rod Chalk, Nicola A Burgess-Brown, Rebecca Sitsapesan, Juha T Huiskonen, Elisabeth P Carpenter
Structure of the polycystic kidney disease TRP channel Polycystin-2 (PC2)
published pages: 114-122, ISSN: 1545-9993, DOI: 10.1038/nsmb.3343
Nature Structural & Molecular Biology 24/2 2019-09-02
2017 Zhaoyang Sun, Kamel El Omari, Xiaoyu Sun, Serban L. Ilca, Abhay Kotecha, David I. Stuart, Minna M. Poranen, Juha T. Huiskonen
Double-stranded RNA virus outer shell assembly by bona fide domain-swapping
published pages: 14814, ISSN: 2041-1723, DOI: 10.1038/ncomms14814
Nature Communications 8 2019-09-02
2016 Renner, Max; Leyrat, Cédric; Huiskonen, Juha T; Paesen, Guido C; Bertinelli, Mattia; Saraiva de Oliveira, Laura Freitas; Grimes, Jonathan M.
Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.12627
eLife 2019-09-02
2016 Sai Li; Ilona Rissanen; Jayna Raghwani; Thomas A. Bowden; Juha T. Huiskonen; Antra Zeltina; Jussi Hepojoki; Karl Harlos; Oliver G. Pybus
A Molecular-Level Account of the Antigenic Hantaviral Surface
published pages: , ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.03.082
Cell Reports 1 2019-09-02
2017 Denis Ptchelkine, Ashley Gillum, Tomohiro Mochizuki, Soizick Lucas-Staat, Ying Liu, Mart Krupovic, Simon E. V. Phillips, David Prangishvili, Juha T. Huiskonen
Unique architecture of thermophilic archaeal virus APBV1 and its genome packaging
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-01668-0
Nature Communications 8/1 2019-09-02
2017 Abhay Kotecha, Quan Wang, Xianchi Dong, Serban L. Ilca, Marina Ondiviela, Rao Zihe, Julian Seago, Bryan Charleston, Elizabeth E. Fry, Nicola G. A. Abrescia, Timothy A. Springer, Juha T. Huiskonen, David I. Stuart
Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus
published pages: 15408, ISSN: 2041-1723, DOI: 10.1038/ncomms15408
Nature Communications 8 2019-09-02
2016 Bitto, David; Halldorsson, Steinar; Huiskonen, Juha T; Caputo, Alessandro
Low pH and Anionic Lipid-dependent Fusion of Uukuniemi Phlebovirus to Liposomes*
published pages: , ISSN: 1083-351X, DOI: 10.1074/jbc.M115.691113
The Journal of Biological Chemistry 2019-09-02
2016 Steinar Halldorsson, Anna-Janina Behrens, Karl Harlos, Juha T. Huiskonen, Richard M Elliott, Max Crispin, Benjamin Brennan, and Thomas A Bowden
Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion
published pages: , ISSN: 1091-6490, DOI: 10.1073/pnas.1603827113
P Natl Acad Sci Usa 2019-09-02
2017 Martin Wilkes, M Gregor Madej, Lydia Kreuter, Daniel Rhinow, Veronika Heinz, Silvia De Sanctis, Sabine Ruppel, Rebecca M Richter, Friederike Joos, Marina Grieben, Ashley C W Pike, Juha T Huiskonen, Elisabeth P Carpenter, Werner Kühlbrandt, Ralph Witzgall, Christine Ziegler
Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2
published pages: 123-130, ISSN: 1545-9993, DOI: 10.1038/nsmb.3357
Nature Structural & Molecular Biology 24/2 2019-09-02

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "BIZEB" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "BIZEB" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

IMPACCT (2019)

Improved Patient Care by Combinatorial Treatment

Read More  

Life-Inspired (2019)

Life-inspired complex molecular systems controlled by enzymatic reaction networks

Read More  

NeuroMag (2019)

The Neurological Basis of the Magnetic Sense

Read More