Opendata, web and dolomites

KGBVIFEF

Utilizing the fusion machinery of Herpes Simplex Virus to unveil the general process of membrane fusion

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 KGBVIFEF project word cloud

Explore the words cloud of the KGBVIFEF project. It provides you a very rough idea of what is the project "KGBVIFEF" about.

thereby    single    electron    glycoproteins    division    competence    contrast    entry    cell    functional    reconstitution    biophysics    temporal    complete    intermediates    cryo    imaging    biochemical    membrane    fluorescence    dynamics    tomography    accomplished    pore    diverse    mechanism    expand    mechanisms    vesicle    sub    structure    question    opportunity    hitherto    machinery    employ    biomolecular    data    length    catalyse    simplex    attachment    spatio    interphase    envelope    volume    found    full    microscopy    modularity    interaction    hsv    ray    nenecessary    averaging    glycoprotein    insights    fusion    triggers    virus    dissect    characterised    ranging    follow    purification    proteins    viruses    herpes    subsequent    classification    host    multidisciplinary    cellular    residing    mechanistic    situ    underlying    mediating    least    trafficking    ill    discrete    advantage    enabled    events    biology    reconstitute    hemifusion    combining    biological    deformation    resolution    basic    conserved    mediated    details    biochemistry    structural    particle    viral    molecular    reveal    species   

Project "KGBVIFEF" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.strubi.ox.ac.uk/profile/benjamin-vollmer
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2017-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 183˙454.00

Map

 Project objective

Membrane fusion is a basic cell biological process found in diverse pathways ranging from vesicle trafficking and cell division to viral host entry. It is mediated by fusion proteins residing in the membrane. The underlying molecular mechanisms are supposed to follow a common order of events, i.e. fusion through hemifusion. Cell entry of Herpes simplex virus-1 (HSV-1) is enabled by glycoproteins residing on the viral envelope membrane. In contrast to other viruses, this is accomplished by different glycoprotein species, mediating together the attachment and subsequent fusion between the viral and host cell membrane. At least four of these proteins are essential for membrane deformation leading to fusion pore formation. In the here proposed project, I will take advantage of the modularity of the HSV-1 fusion machinery to dissect this process into discrete steps which I will analyse in situ at molecular resolution to determine the molecular details of membrane fusion. To do so, I will employ a multidisciplinary approach combining methods and data from structural biology, biochemistry as well as biophysics and molecular dynamics to solve the mechanistic details of a cell biological question. This includes fluorescence and cryo electron microscopy and tomography full-length membrane glycoprotein purification and biochemical reconstitution methods, biomolecular interaction and structural X-ray analysis, sub-volume averaging and classification as well as single particle imaging. To find the nenecessary triggers for fusion I will reconstitute the complete fusion system and thereby reveal the spatio-temporal changes that catalyse the fusion process. Taken together this structure-functional study will enable insights into hitherto ill-characterised intermediates in the conserved mechanism of membrane fusion. This project is a great opportunity to expand my research competence at the interphase of different fields ranging from cellular and structural biology to biophysics.

 Publications

year authors and title journal last update
List of publications.
2016 Tzviya Zeev-Ben-Mordehai, Daven Vasishtan, Anna Hernández Durán, Benjamin Vollmer, Paul White, Arun Prasad Pandurangan, C. Alistair Siebert, Maya Topf, Kay Grünewald
Two distinct trimeric conformations of natively membrane-anchored full-length herpes simplex virus 1 glycoprotein B
published pages: 4176-4181, ISSN: 0027-8424, DOI: 10.1073/pnas.1523234113
Proceedings of the National Academy of Sciences 113/15 2019-07-23

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "KGBVIFEF" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "KGBVIFEF" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MBL-Fermions (2020)

Probing many-body localization dynamics using ultracold fermions in an optical lattice

Read More  

ProgNanoRobot (2019)

Programmable NanoRobotics for Controlled Manipulation of Molecular Cargoes

Read More  

PARTOWNEU (2019)

The dark side of partial ownership and financial investment in Europe: What price to pay for consumers and society?

Read More