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KGBVIFEF

Utilizing the fusion machinery of Herpes Simplex Virus to unveil the general process of membrane fusion

Total Cost €

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EC-Contrib. €

0

Partnership

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 KGBVIFEF project word cloud

Explore the words cloud of the KGBVIFEF project. It provides you a very rough idea of what is the project "KGBVIFEF" about.

accomplished    trafficking    dissect    vesicle    insights    reconstitute    species    mechanistic    triggers    found    ray    cryo    biophysics    combining    thereby    imaging    structure    electron    attachment    reconstitution    biological    biochemical    opportunity    glycoproteins    single    purification    data    division    virus    hemifusion    glycoprotein    mediating    full    mechanism    reveal    enabled    resolution    pore    proteins    diverse    complete    follow    underlying    spatio    cellular    structural    hitherto    microscopy    interphase    modularity    discrete    ranging    tomography    employ    cell    multidisciplinary    expand    fluorescence    classification    host    biochemistry    mechanisms    intermediates    viral    particle    functional    details    nenecessary    subsequent    events    ill    interaction    dynamics    averaging    question    herpes    envelope    catalyse    mediated    temporal    biology    advantage    deformation    sub    situ    simplex    conserved    entry    biomolecular    competence    viruses    least    basic    machinery    volume    molecular    fusion    residing    length    membrane    hsv    contrast    characterised   

Project "KGBVIFEF" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.strubi.ox.ac.uk/profile/benjamin-vollmer
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2017-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 183˙454.00

Map

 Project objective

Membrane fusion is a basic cell biological process found in diverse pathways ranging from vesicle trafficking and cell division to viral host entry. It is mediated by fusion proteins residing in the membrane. The underlying molecular mechanisms are supposed to follow a common order of events, i.e. fusion through hemifusion. Cell entry of Herpes simplex virus-1 (HSV-1) is enabled by glycoproteins residing on the viral envelope membrane. In contrast to other viruses, this is accomplished by different glycoprotein species, mediating together the attachment and subsequent fusion between the viral and host cell membrane. At least four of these proteins are essential for membrane deformation leading to fusion pore formation. In the here proposed project, I will take advantage of the modularity of the HSV-1 fusion machinery to dissect this process into discrete steps which I will analyse in situ at molecular resolution to determine the molecular details of membrane fusion. To do so, I will employ a multidisciplinary approach combining methods and data from structural biology, biochemistry as well as biophysics and molecular dynamics to solve the mechanistic details of a cell biological question. This includes fluorescence and cryo electron microscopy and tomography full-length membrane glycoprotein purification and biochemical reconstitution methods, biomolecular interaction and structural X-ray analysis, sub-volume averaging and classification as well as single particle imaging. To find the nenecessary triggers for fusion I will reconstitute the complete fusion system and thereby reveal the spatio-temporal changes that catalyse the fusion process. Taken together this structure-functional study will enable insights into hitherto ill-characterised intermediates in the conserved mechanism of membrane fusion. This project is a great opportunity to expand my research competence at the interphase of different fields ranging from cellular and structural biology to biophysics.

 Publications

year authors and title journal last update
List of publications.
2016 Tzviya Zeev-Ben-Mordehai, Daven Vasishtan, Anna Hernández Durán, Benjamin Vollmer, Paul White, Arun Prasad Pandurangan, C. Alistair Siebert, Maya Topf, Kay Grünewald
Two distinct trimeric conformations of natively membrane-anchored full-length herpes simplex virus 1 glycoprotein B
published pages: 4176-4181, ISSN: 0027-8424, DOI: 10.1073/pnas.1523234113
Proceedings of the National Academy of Sciences 113/15 2019-07-23

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