Opendata, web and dolomites

METALS SIGNED

Formation and Characterization of Protein Post-Translational Modifications and Assessment of Cellular Responses by Application of Metals in Biological Systems

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 METALS project word cloud

Explore the words cloud of the METALS project. It provides you a very rough idea of what is the project "METALS" about.

independent    cosmetics    binding    terminal    amounts    viewed    cells    human    examine    reaction    inherent    reactions    mass    cell    area    chemistry    biochemical    spectrometry    confocal    proteins    produces    food    nanoparticles    efficient    limit    phosphatases    living    elucidate    compounds    attempt    first    histidine    labile    multitude    amidation    minimize    peptide    attractive    enzyme    presently    pharmaceutical    inhibition    amidated    society    proteome    peptides    microscopy    suggested    light    bias    phospho    limitations    context    shed    neutral    action    biologically    diseases    poses    exposed    enzymes    acute    subsequent    possibility    irradiation    biological    reveal    risks    nano    artificially    gallium    phosphopeptide    recombinant    packages    particles    uv    acid    uranyl    handful    industry    metals    data    molecular    academia    synthesize    enrichment    assays    metal    disease    purpose    diversity    humans    inefficient    functions    neural    lysis    phosphorylated    plan    parkinson    sized   

Project "METALS" data sheet

The following table provides information about the project.

Coordinator
SYDDANSK UNIVERSITET 

Organization address
address: CAMPUSVEJ 55
city: ODENSE M
postcode: 5230
website: www.sdu.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Project website http://www.sdu.dk/en/om_sdu/institutter_centre/bmb_biokemi_og_molekylaer_biologi/forskning/forskningsenheder/frankkjeldsen
 Total cost 1˙798˙750 €
 EC max contribution 1˙798˙750 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    SYDDANSK UNIVERSITET DK (ODENSE M) coordinator 1˙798˙750.00

Map

 Project objective

The chemistry of metals is rich and viewed in a biological context its diversity is crucial for a multitude of molecular functions in the living cell. Many of these reactions are very attractive to both academia and industry. In this proposal, I plan to develop novel applications of metal compounds to solve immediate challenges in mass spectrometry-based proteome research, but also will assess the potential risks of using nano-sized metals in our society. First, it is important to develop an efficient enzyme-independent method to synthesize large amounts of biologically relevant C-terminal amidated peptides. Presently, C-terminal peptide amidation poses a challenge in pharmaceutical production due to limitations of the two enzymes used for this purpose. The suggested approach in METALS will examine the specific binding of uranyl to artificially phosphorylated recombinant peptides. Data reveal that subsequent UV irradiation produces C-terminal amidated peptides. I will attempt to minimize the bias inherent in current phosphopeptide analysis, which comes from inefficient inhibition of phosphatases during cell lysis. Application of a recently developed gallium complex during cell lysis should limit the extent of this bias by binding phosphorylated proteins. The neutral conditions involved with the gallium complex reaction should also facilitate the possibility of enrichment of acid labile phospho-histidine peptides of which only a handful have been characterized. Finally, humans are now exposed to increasing amounts of artificially nano-metals applied via consumer products, food packages, and cosmetics. I will investigate this problem using advanced mass spectrometry, confocal microscopy, and biochemical assays of the response of human neural cells to nano-metal particles. The particular focus area will be to elucidate whether the action of nanoparticles in human neural cells may shed new light on understanding of diseases like Parkinson´s disease.

 Publications

year authors and title journal last update
List of publications.
2020 Anja M. Billing, Kristina B. Knudsen, Andrew J. Chetwynd, Laura-Jayne A. Ellis, Selina V.Y. Tang, Trine Berthing, HÃ¥kan Wallin, Iseult Lynch, Ulla Vogel, Frank Kjeldsen
Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles
published pages: , ISSN: 1936-0851, DOI: 10.1021/acsnano.9b08818
ACS Nano 2020-03-24
2020 Julia A. Bubis, Daria S. Spasskaya, Vladimir A. Gorshkov, Frank Kjeldsen, Aleksandra M. Kofanova, Dmitry S. Lekanov, Mikhail V. Gorshkov, Vadim L. Karpov, Irina A. Tarasova, Dmitry S. Karpov
Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy
published pages: , ISSN: 0175-7598, DOI: 10.1007/s00253-020-10518-x
Applied Microbiology and Biotechnology 2020-03-24
2020 Mark V. Ivanov, Julia A. Bubis, Vladimir Gorshkov, Irina A. Tarasova, Lev I. Levitsky, Anna A. Lobas, Elizaveta M. Solovyeva, Marina L. Pridatchenko, Frank Kjeldsen, Mikhail V. Gorshkov
DirectMS1: MS/MS-Free Identification of 1000 Proteins of Cellular Proteomes in 5 Minutes
published pages: , ISSN: 0003-2700, DOI: 10.1021/acs.analchem.9b05095
Analytical Chemistry 2020-03-11
2019 Asif Manzoor Khan, Barbara Korzeniowska, Vladimir Gorshkov, Muhammad Tahir, Henrik Schrøder, Lilian Skytte, Kaare Lund Rasmussen, Surabhi Khandige, Jakob Møller-Jensen, Frank Kjeldsen
Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an in vitro human blood-brain barrier model
published pages: 221-239, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1540728
Nanotoxicology 13/2 2020-03-11
2019 Roberto Gonzalez-Pizarro, Graziella Parrotta, Rodrigo Vera, Elena Sánchez-López, Ruth Galindo, Frank Kjeldsen, Josefa Badia, Laura Baldoma, Marta Espina, María L García
Ocular penetration of fluorometholone-loaded PEG-PLGA nanoparticles functionalized with cell-penetrating peptides
published pages: 3089-3104, ISSN: 1743-5889, DOI: 10.2217/nnm-2019-0201
Nanomedicine 14/23 2020-03-11
2019 Vladimir Gorshkov, Julia A. Bubis, Elizaveta M. Solovyeva, Mikhail V. Gorshkov, Frank Kjeldsen
Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution
published pages: 1089-1098, ISSN: 2051-8153, DOI: 10.1039/c8en01054d
Environmental Science: Nano 6/4 2020-03-11
2019 Diana Paola Gómez-Mendoza, Fúlvia Dias Marques, Marcella Nunes Melo-Braga, Richard R. Sprenger, Rubén Dario Sinisterra, Frank Kjeldsen, Robson Augusto Santos, Thiago Verano-Braga
Angiotensin-(1-7) oral treatment after experimental myocardial infarction leads to downregulation of CXCR4
published pages: 103486, ISSN: 1874-3919, DOI: 10.1016/j.jprot.2019.103486
Journal of Proteomics 208 2020-03-11
2019 Lucas Rodrigues-Ribeiro, Marcella Nunes Melo-Braga, Frank Kjeldsen, Diana Paola Gómez-Mendoza, Thiago Verano-Braga
Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
published pages: 51-59, ISSN: 0003-2697, DOI: 10.1016/j.ab.2019.05.006
Analytical Biochemistry 578 2020-03-11
2016 Vladimir Gorshkov, Stéphanie Yuki Kolbeck Hotta, Thiago Verano-Braga, Frank Kjeldsen
Peptide de novo sequencing of mixture tandem mass spectra
published pages: 2470-2479, ISSN: 1615-9853, DOI: 10.1002/pmic.201500549
PROTEOMICS 16/18 2019-05-30
2017 Rasmus Linde Bang Elnegaard, Niels Erik Møllegaard, Qiang Zhang, Frank Kjeldsen, Thomas J.D. Jørgensen
Uranyl photocleavage of phosphopeptides yields truncated C-terminally amidated peptide products
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201700103
ChemBioChem 2019-05-30
2018 Irina A Tarasova, Alesya V Tereshkova, Anna A Lobas, Elizaveta M Solovyeva, Alena S Sidorenko, Vladimir Gorshkov, Frank Kjeldsen, Julia A Bubis, Mark V Ivanov, Irina Y Ilina, Sergei A Moshkovskii, Peter M Chumakov, Mikhail V Gorshkov
Comparative proteomics as a tool for identifying specific alterations within interferon response pathways in human glioblastoma multiforme cells
published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.22751
Oncotarget 9/2 2019-05-30
2017 Renata Rank Miranda, Arandi Ginane Bezerra Jr, Ciro Alberto Oliveira Ribeiro, Marco Antônio Ferreira Randi, Carmen Lúcia Voigt, Lilian Skytte, Kaare Lund Rasmussen, Frank Kjeldsen, Francisco Filipak Neto
Toxicological interactions of silver nanoparticles and non-essential metals in human hepatocarcinoma cell line
published pages: 134-143, ISSN: 0887-2333, DOI: 10.1016/j.tiv.2017.01.003
Toxicology in Vitro 40 2019-05-30
2018 Renata Rank Miranda, Vladimir Gorshkov, Barbara Korzeniowska, Stefan J. Kempf, Francisco Filipak Neto, Frank Kjeldsen
Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells
published pages: 781-795, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1489987
Nanotoxicology 12/7 2019-04-18

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "METALS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "METALS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

ChaperoneRegulome (2020)

ChaperoneRegulome: Understanding cell-type-specificity of chaperone regulation

Read More  

SWIP (2019)

Extended short-wave infrared pulsed fibre laser

Read More  

MOCHA (2019)

Understanding and leveraging ‘moments of change’ for pro-environmental behaviour shifts

Read More