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METALS SIGNED

Formation and Characterization of Protein Post-Translational Modifications and Assessment of Cellular Responses by Application of Metals in Biological Systems

Total Cost €

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EC-Contrib. €

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Partnership

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 METALS project word cloud

Explore the words cloud of the METALS project. It provides you a very rough idea of what is the project "METALS" about.

plan    elucidate    irradiation    proteome    disease    acute    mass    bias    particles    parkinson    terminal    diversity    living    amidation    gallium    reveal    phosphopeptide    neutral    nanoparticles    labile    functions    society    chemistry    independent    phosphatases    presently    nano    risks    first    microscopy    amounts    spectrometry    suggested    industry    peptide    data    pharmaceutical    humans    human    packages    cosmetics    lysis    acid    amidated    multitude    proteins    binding    area    biologically    compounds    assays    limit    cells    minimize    context    possibility    handful    uranyl    artificially    attempt    academia    biochemical    molecular    peptides    synthesize    recombinant    phospho    shed    viewed    limitations    food    efficient    enzyme    attractive    inefficient    subsequent    cell    enrichment    metal    reactions    exposed    inhibition    inherent    neural    poses    reaction    enzymes    produces    sized    metals    examine    purpose    light    action    phosphorylated    diseases    confocal    histidine    biological    uv   

Project "METALS" data sheet

The following table provides information about the project.

Coordinator
SYDDANSK UNIVERSITET 

Organization address
address: CAMPUSVEJ 55
city: ODENSE M
postcode: 5230
website: www.sdu.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Project website http://www.sdu.dk/en/om_sdu/institutter_centre/bmb_biokemi_og_molekylaer_biologi/forskning/forskningsenheder/frankkjeldsen
 Total cost 1˙798˙750 €
 EC max contribution 1˙798˙750 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    SYDDANSK UNIVERSITET DK (ODENSE M) coordinator 1˙798˙750.00

Map

 Project objective

The chemistry of metals is rich and viewed in a biological context its diversity is crucial for a multitude of molecular functions in the living cell. Many of these reactions are very attractive to both academia and industry. In this proposal, I plan to develop novel applications of metal compounds to solve immediate challenges in mass spectrometry-based proteome research, but also will assess the potential risks of using nano-sized metals in our society. First, it is important to develop an efficient enzyme-independent method to synthesize large amounts of biologically relevant C-terminal amidated peptides. Presently, C-terminal peptide amidation poses a challenge in pharmaceutical production due to limitations of the two enzymes used for this purpose. The suggested approach in METALS will examine the specific binding of uranyl to artificially phosphorylated recombinant peptides. Data reveal that subsequent UV irradiation produces C-terminal amidated peptides. I will attempt to minimize the bias inherent in current phosphopeptide analysis, which comes from inefficient inhibition of phosphatases during cell lysis. Application of a recently developed gallium complex during cell lysis should limit the extent of this bias by binding phosphorylated proteins. The neutral conditions involved with the gallium complex reaction should also facilitate the possibility of enrichment of acid labile phospho-histidine peptides of which only a handful have been characterized. Finally, humans are now exposed to increasing amounts of artificially nano-metals applied via consumer products, food packages, and cosmetics. I will investigate this problem using advanced mass spectrometry, confocal microscopy, and biochemical assays of the response of human neural cells to nano-metal particles. The particular focus area will be to elucidate whether the action of nanoparticles in human neural cells may shed new light on understanding of diseases like Parkinson´s disease.

 Publications

year authors and title journal last update
List of publications.
2020 Anja M. Billing, Kristina B. Knudsen, Andrew J. Chetwynd, Laura-Jayne A. Ellis, Selina V.Y. Tang, Trine Berthing, HÃ¥kan Wallin, Iseult Lynch, Ulla Vogel, Frank Kjeldsen
Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles
published pages: , ISSN: 1936-0851, DOI: 10.1021/acsnano.9b08818
ACS Nano 2020-03-24
2020 Julia A. Bubis, Daria S. Spasskaya, Vladimir A. Gorshkov, Frank Kjeldsen, Aleksandra M. Kofanova, Dmitry S. Lekanov, Mikhail V. Gorshkov, Vadim L. Karpov, Irina A. Tarasova, Dmitry S. Karpov
Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy
published pages: , ISSN: 0175-7598, DOI: 10.1007/s00253-020-10518-x
Applied Microbiology and Biotechnology 2020-03-24
2020 Mark V. Ivanov, Julia A. Bubis, Vladimir Gorshkov, Irina A. Tarasova, Lev I. Levitsky, Anna A. Lobas, Elizaveta M. Solovyeva, Marina L. Pridatchenko, Frank Kjeldsen, Mikhail V. Gorshkov
DirectMS1: MS/MS-Free Identification of 1000 Proteins of Cellular Proteomes in 5 Minutes
published pages: , ISSN: 0003-2700, DOI: 10.1021/acs.analchem.9b05095
Analytical Chemistry 2020-03-11
2019 Asif Manzoor Khan, Barbara Korzeniowska, Vladimir Gorshkov, Muhammad Tahir, Henrik Schrøder, Lilian Skytte, Kaare Lund Rasmussen, Surabhi Khandige, Jakob Møller-Jensen, Frank Kjeldsen
Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an in vitro human blood-brain barrier model
published pages: 221-239, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1540728
Nanotoxicology 13/2 2020-03-11
2019 Roberto Gonzalez-Pizarro, Graziella Parrotta, Rodrigo Vera, Elena Sánchez-López, Ruth Galindo, Frank Kjeldsen, Josefa Badia, Laura Baldoma, Marta Espina, María L García
Ocular penetration of fluorometholone-loaded PEG-PLGA nanoparticles functionalized with cell-penetrating peptides
published pages: 3089-3104, ISSN: 1743-5889, DOI: 10.2217/nnm-2019-0201
Nanomedicine 14/23 2020-03-11
2019 Vladimir Gorshkov, Julia A. Bubis, Elizaveta M. Solovyeva, Mikhail V. Gorshkov, Frank Kjeldsen
Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution
published pages: 1089-1098, ISSN: 2051-8153, DOI: 10.1039/c8en01054d
Environmental Science: Nano 6/4 2020-03-11
2019 Diana Paola Gómez-Mendoza, Fúlvia Dias Marques, Marcella Nunes Melo-Braga, Richard R. Sprenger, Rubén Dario Sinisterra, Frank Kjeldsen, Robson Augusto Santos, Thiago Verano-Braga
Angiotensin-(1-7) oral treatment after experimental myocardial infarction leads to downregulation of CXCR4
published pages: 103486, ISSN: 1874-3919, DOI: 10.1016/j.jprot.2019.103486
Journal of Proteomics 208 2020-03-11
2019 Lucas Rodrigues-Ribeiro, Marcella Nunes Melo-Braga, Frank Kjeldsen, Diana Paola Gómez-Mendoza, Thiago Verano-Braga
Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
published pages: 51-59, ISSN: 0003-2697, DOI: 10.1016/j.ab.2019.05.006
Analytical Biochemistry 578 2020-03-11
2016 Vladimir Gorshkov, Stéphanie Yuki Kolbeck Hotta, Thiago Verano-Braga, Frank Kjeldsen
Peptide de novo sequencing of mixture tandem mass spectra
published pages: 2470-2479, ISSN: 1615-9853, DOI: 10.1002/pmic.201500549
PROTEOMICS 16/18 2019-05-30
2017 Rasmus Linde Bang Elnegaard, Niels Erik Møllegaard, Qiang Zhang, Frank Kjeldsen, Thomas J.D. Jørgensen
Uranyl photocleavage of phosphopeptides yields truncated C-terminally amidated peptide products
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201700103
ChemBioChem 2019-05-30
2018 Irina A Tarasova, Alesya V Tereshkova, Anna A Lobas, Elizaveta M Solovyeva, Alena S Sidorenko, Vladimir Gorshkov, Frank Kjeldsen, Julia A Bubis, Mark V Ivanov, Irina Y Ilina, Sergei A Moshkovskii, Peter M Chumakov, Mikhail V Gorshkov
Comparative proteomics as a tool for identifying specific alterations within interferon response pathways in human glioblastoma multiforme cells
published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.22751
Oncotarget 9/2 2019-05-30
2017 Renata Rank Miranda, Arandi Ginane Bezerra Jr, Ciro Alberto Oliveira Ribeiro, Marco Antônio Ferreira Randi, Carmen Lúcia Voigt, Lilian Skytte, Kaare Lund Rasmussen, Frank Kjeldsen, Francisco Filipak Neto
Toxicological interactions of silver nanoparticles and non-essential metals in human hepatocarcinoma cell line
published pages: 134-143, ISSN: 0887-2333, DOI: 10.1016/j.tiv.2017.01.003
Toxicology in Vitro 40 2019-05-30
2018 Renata Rank Miranda, Vladimir Gorshkov, Barbara Korzeniowska, Stefan J. Kempf, Francisco Filipak Neto, Frank Kjeldsen
Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells
published pages: 781-795, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1489987
Nanotoxicology 12/7 2019-04-18

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