Explore the words cloud of the METALS project. It provides you a very rough idea of what is the project "METALS" about.
The following table provides information about the project.
Coordinator |
SYDDANSK UNIVERSITET
Organization address contact info |
Coordinator Country | Denmark [DK] |
Project website | http://www.sdu.dk/en/om_sdu/institutter_centre/bmb_biokemi_og_molekylaer_biologi/forskning/forskningsenheder/frankkjeldsen |
Total cost | 1˙798˙750 € |
EC max contribution | 1˙798˙750 € (100%) |
Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
Code Call | ERC-2014-CoG |
Funding Scheme | ERC-COG |
Starting year | 2015 |
Duration (year-month-day) | from 2015-09-01 to 2021-02-28 |
Take a look of project's partnership.
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1 | SYDDANSK UNIVERSITET | DK (ODENSE M) | coordinator | 1˙798˙750.00 |
The chemistry of metals is rich and viewed in a biological context its diversity is crucial for a multitude of molecular functions in the living cell. Many of these reactions are very attractive to both academia and industry. In this proposal, I plan to develop novel applications of metal compounds to solve immediate challenges in mass spectrometry-based proteome research, but also will assess the potential risks of using nano-sized metals in our society. First, it is important to develop an efficient enzyme-independent method to synthesize large amounts of biologically relevant C-terminal amidated peptides. Presently, C-terminal peptide amidation poses a challenge in pharmaceutical production due to limitations of the two enzymes used for this purpose. The suggested approach in METALS will examine the specific binding of uranyl to artificially phosphorylated recombinant peptides. Data reveal that subsequent UV irradiation produces C-terminal amidated peptides. I will attempt to minimize the bias inherent in current phosphopeptide analysis, which comes from inefficient inhibition of phosphatases during cell lysis. Application of a recently developed gallium complex during cell lysis should limit the extent of this bias by binding phosphorylated proteins. The neutral conditions involved with the gallium complex reaction should also facilitate the possibility of enrichment of acid labile phospho-histidine peptides of which only a handful have been characterized. Finally, humans are now exposed to increasing amounts of artificially nano-metals applied via consumer products, food packages, and cosmetics. I will investigate this problem using advanced mass spectrometry, confocal microscopy, and biochemical assays of the response of human neural cells to nano-metal particles. The particular focus area will be to elucidate whether the action of nanoparticles in human neural cells may shed new light on understanding of diseases like Parkinson´s disease.
year | authors and title | journal | last update |
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2020 |
Anja M. Billing, Kristina B. Knudsen, Andrew J. Chetwynd, Laura-Jayne A. Ellis, Selina V.Y. Tang, Trine Berthing, HÃ¥kan Wallin, Iseult Lynch, Ulla Vogel, Frank Kjeldsen Fast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles published pages: , ISSN: 1936-0851, DOI: 10.1021/acsnano.9b08818 |
ACS Nano | 2020-03-24 |
2020 |
Julia A. Bubis, Daria S. Spasskaya, Vladimir A. Gorshkov, Frank Kjeldsen, Aleksandra M. Kofanova, Dmitry S. Lekanov, Mikhail V. Gorshkov, Vadim L. Karpov, Irina A. Tarasova, Dmitry S. Karpov Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy published pages: , ISSN: 0175-7598, DOI: 10.1007/s00253-020-10518-x |
Applied Microbiology and Biotechnology | 2020-03-24 |
2020 |
Mark V. Ivanov, Julia A. Bubis, Vladimir Gorshkov, Irina A. Tarasova, Lev I. Levitsky, Anna A. Lobas, Elizaveta M. Solovyeva, Marina L. Pridatchenko, Frank Kjeldsen, Mikhail V. Gorshkov DirectMS1: MS/MS-Free Identification of 1000 Proteins of Cellular Proteomes in 5 Minutes published pages: , ISSN: 0003-2700, DOI: 10.1021/acs.analchem.9b05095 |
Analytical Chemistry | 2020-03-11 |
2019 |
Asif Manzoor Khan, Barbara Korzeniowska, Vladimir Gorshkov, Muhammad Tahir, Henrik Schrøder, Lilian Skytte, Kaare Lund Rasmussen, Surabhi Khandige, Jakob Møller-Jensen, Frank Kjeldsen Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an in vitro human blood-brain barrier model published pages: 221-239, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1540728 |
Nanotoxicology 13/2 | 2020-03-11 |
2019 |
Roberto Gonzalez-Pizarro, Graziella Parrotta, Rodrigo Vera, Elena Sánchez-López, Ruth Galindo, Frank Kjeldsen, Josefa Badia, Laura Baldoma, Marta Espina, MarÃa L GarcÃa Ocular penetration of fluorometholone-loaded PEG-PLGA nanoparticles functionalized with cell-penetrating peptides published pages: 3089-3104, ISSN: 1743-5889, DOI: 10.2217/nnm-2019-0201 |
Nanomedicine 14/23 | 2020-03-11 |
2019 |
Vladimir Gorshkov, Julia A. Bubis, Elizaveta M. Solovyeva, Mikhail V. Gorshkov, Frank Kjeldsen Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution published pages: 1089-1098, ISSN: 2051-8153, DOI: 10.1039/c8en01054d |
Environmental Science: Nano 6/4 | 2020-03-11 |
2019 |
Diana Paola Gómez-Mendoza, Fúlvia Dias Marques, Marcella Nunes Melo-Braga, Richard R. Sprenger, Rubén Dario Sinisterra, Frank Kjeldsen, Robson Augusto Santos, Thiago Verano-Braga Angiotensin-(1-7) oral treatment after experimental myocardial infarction leads to downregulation of CXCR4 published pages: 103486, ISSN: 1874-3919, DOI: 10.1016/j.jprot.2019.103486 |
Journal of Proteomics 208 | 2020-03-11 |
2019 |
Lucas Rodrigues-Ribeiro, Marcella Nunes Melo-Braga, Frank Kjeldsen, Diana Paola Gómez-Mendoza, Thiago Verano-Braga Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics published pages: 51-59, ISSN: 0003-2697, DOI: 10.1016/j.ab.2019.05.006 |
Analytical Biochemistry 578 | 2020-03-11 |
2016 |
Vladimir Gorshkov, Stéphanie Yuki Kolbeck Hotta, Thiago Verano-Braga, Frank Kjeldsen Peptide de novo sequencing of mixture tandem mass spectra published pages: 2470-2479, ISSN: 1615-9853, DOI: 10.1002/pmic.201500549 |
PROTEOMICS 16/18 | 2019-05-30 |
2017 |
Rasmus Linde Bang Elnegaard, Niels Erik Møllegaard, Qiang Zhang, Frank Kjeldsen, Thomas J.D. Jørgensen Uranyl photocleavage of phosphopeptides yields truncated C-terminally amidated peptide products published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201700103 |
ChemBioChem | 2019-05-30 |
2018 |
Irina A Tarasova, Alesya V Tereshkova, Anna A Lobas, Elizaveta M Solovyeva, Alena S Sidorenko, Vladimir Gorshkov, Frank Kjeldsen, Julia A Bubis, Mark V Ivanov, Irina Y Ilina, Sergei A Moshkovskii, Peter M Chumakov, Mikhail V Gorshkov Comparative proteomics as a tool for identifying specific alterations within interferon response pathways in human glioblastoma multiforme cells published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.22751 |
Oncotarget 9/2 | 2019-05-30 |
2017 |
Renata Rank Miranda, Arandi Ginane Bezerra Jr, Ciro Alberto Oliveira Ribeiro, Marco Antônio Ferreira Randi, Carmen Lúcia Voigt, Lilian Skytte, Kaare Lund Rasmussen, Frank Kjeldsen, Francisco Filipak Neto Toxicological interactions of silver nanoparticles and non-essential metals in human hepatocarcinoma cell line published pages: 134-143, ISSN: 0887-2333, DOI: 10.1016/j.tiv.2017.01.003 |
Toxicology in Vitro 40 | 2019-05-30 |
2018 |
Renata Rank Miranda, Vladimir Gorshkov, Barbara Korzeniowska, Stefan J. Kempf, Francisco Filipak Neto, Frank Kjeldsen Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells published pages: 781-795, ISSN: 1743-5390, DOI: 10.1080/17435390.2018.1489987 |
Nanotoxicology 12/7 | 2019-04-18 |
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