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P-MEM-NMR SIGNED

Structure of paramagnetic integral membrane metalloproteins by MAS-NMR

Total Cost €

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EC-Contrib. €

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Partnership

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 P-MEM-NMR project word cloud

Explore the words cloud of the P-MEM-NMR project. It provides you a very rough idea of what is the project "P-MEM-NMR" about.

tremendous    resolution    leap    cellular    combination    integral    fine    life    nickel    magnetic    calculation    sciences    single    ion    membrane    nuclei    mas    environment    structures    goals    transporters    electronic    elucidation    extend    samples    redox    metalloenzymes    series    applicable    faster    cobalt    biological    labeling    molecular    powerful    combining    crystal    thereby    surrounding    lipid    broadly    isotopic    outlined    metalloproteins    rates    magic    nuclear    innovative    macromolecules    angle    tool    unsolved    sensitivity    crystals    structural    homeostasis    leverage    frontier    biochemical    metal    industry    characterization    transport    atomic    yield    experiments    reactions    relationships    pioneered    details    derives    diffraction    global    breakthrough    structure    protocols    applicability    resonance    nmr    energy    ray    respect    chemical    proteins    dynamics    iron    spinning    paramagnetic    forefront    materials    environments    easily    ions    largely   

Project "P-MEM-NMR" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://perso.ens-lyon.fr/guido.pintacuda/guidosgroup/Research.html
 Total cost 2˙499˙375 €
 EC max contribution 2˙499˙375 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 2˙499˙375.00

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 Project objective

Integral membrane metalloproteins are involved in the transport and homeostasis of metal ions, as well as in key redox reactions that have a tremendous impact on many fields within life sciences, environment, energy, and industry. Most of our understanding of fine details of biochemical processes derives from atomic or molecular structures obtained by diffraction methods on single crystal samples. However, in the case of integral membrane systems, single crystals large enough for X-ray diffraction cannot be easily obtained, and the problem of structure elucidation is largely unsolved. We have recently pioneered a breakthrough approach using Magic-Angle Spinning Nuclear Magnetic Resonance (MAS-NMR) for the atomic-level characterization of paramagnetic materials and complex biological macromolecules. The proposed project aims to leverage these new advances through a series of new concepts i) to improve the resolution and sensitivity of MAS-NMR from nuclei surrounding a paramagnetic metal ion, such as e.g. cobalt, nickel and iron, and ii) to extend its applicability to large integral membrane proteins in lipid membrane environments. With these methods, we will enable the determination of structure-activity relationships in integral membrane metalloenzymes and transporters, by combining the calculation of global structure and dynamics with measurement of the electronic features of metal ions. These goals require a leap forward with respect to today’s protocols, and we propose to achieve this through a combination of innovative NMR experiments and isotopic labeling, faster MAS rates and high magnetic fields. As outlined here, the approaches go well beyond the frontier of current research. The project will yield a broadly applicable method for the structural characterization of essential cellular processes and thereby will provide a powerful tool to solve challenges at the forefront of molecular and chemical sciences today.

 Publications

year authors and title journal last update
List of publications.
2018 Tobias Schubeis, Tanguy Le Marchand, Loren B. Andreas, Guido Pintacuda
1 H magic-angle spinning NMR evolves as a powerful new tool for membrane proteins
published pages: 140-152, ISSN: 1090-7807, DOI: 10.1016/j.jmr.2017.11.014
Journal of Magnetic Resonance 287 2019-06-07
2016 Jan Stanek, Loren B. Andreas, Kristaps Jaudzems, Diane Cala, Daniela Lalli, Andrea Bertarello, Tobias Schubeis, Inara Akopjana, Svetlana Kotelovica, Kaspars Tars, Andrea Pica, Serena Leone, Delia Picone, Zhi-Qiang Xu, Nicholas E. Dixon, Denis Martinez, Mélanie Berbon, Nadia El Mammeri, Abdelmajid Noubhani, Sven Saupe, Birgit Habenstein, Antoine Loquet, Guido Pintacuda
NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils
published pages: 15504-15509, ISSN: 1433-7851, DOI: 10.1002/anie.201607084
Angewandte Chemie International Edition 55/50 2019-06-07
2018 Antoine Loquet, Nadia El Mammeri, Jan Stanek, Mélanie Berbon, Benjamin Bardiaux, Guido Pintacuda, Birgit Habenstein
3D structure determination of amyloid fibrils using solid-state NMR spectroscopy
published pages: , ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2018.03.014
Methods 2019-06-07
2017 Diane Cala-De Paepe, Jan Stanek, Kristaps Jaudzems, Kaspars Tars, Loren B. Andreas, Guido Pintacuda
Is protein deuteration beneficial for proton detected solid-state NMR at and above 100 kHz magic-angle spinning?
published pages: 126-136, ISSN: 0926-2040, DOI: 10.1016/j.ssnmr.2017.07.004
Solid State Nuclear Magnetic Resonance 87 2019-06-07
2018 Kevin J. Sanders, Andrew J. Pell, Sebastian Wegner, Clare P. Grey, Guido Pintacuda
Broadband MAS NMR spectroscopy in the low-power limit
published pages: 29-37, ISSN: 0009-2614, DOI: 10.1016/j.cplett.2018.01.028
Chemical Physics Letters 697 2019-06-07
2018 Tanguy Le Marchand, Matteo de Rosa, Nicola Salvi, Benedetta Maria Sala, Loren B. Andreas, Emeline Barbet-Massin, Pietro Sormanni, Alberto Barbiroli, Riccardo Porcari, Cristiano Sousa Mota, Daniele de Sanctis, Martino Bolognesi, Lyndon Emsley, Vittorio Bellotti, Martin Blackledge, Carlo Camilloni, Guido Pintacuda, Stefano Ricagno
Conformational dynamics in crystals reveal the molecular bases for D76N beta-2 microglobulin aggregation propensity
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04078-y
Nature Communications 9/1 2019-06-07
2016 Loren B. Andreas, Kristaps Jaudzems, Jan Stanek, Daniela Lalli, Andrea Bertarello, Tanguy Le Marchand, Diane Cala-De Paepe, Svetlana Kotelovica, Inara Akopjana, Benno Knott, Sebastian Wegner, Frank Engelke, Anne Lesage, Lyndon Emsley, Kaspars Tars, Torsten Herrmann, Guido Pintacuda
Structure of fully protonated proteins by proton-detected magic-angle spinning NMR
published pages: 9187-9192, ISSN: 0027-8424, DOI: 10.1073/pnas.1602248113
Proceedings of the National Academy of Sciences 113/33 2019-06-07
2017 Andrea Bertarello, Tobias Schubeis, Carmelo Fuccio, Enrico Ravera, Marco Fragai, Giacomo Parigi, Lyndon Emsley, Guido Pintacuda, Claudio Luchinat
Paramagnetic Properties of a Crystalline Iron–Sulfur Protein by Magic-Angle Spinning NMR Spectroscopy
published pages: 6624-6629, ISSN: 0020-1669, DOI: 10.1021/acs.inorgchem.7b00674
Inorganic Chemistry 56/11 2019-06-07
2017 Mukul G. Jain, Daniela Lalli, Jan Stanek, Chandrakala Gowda, Satya Prakash, Tom S. Schwarzer, Tobias Schubeis, Kathrin Castiglione, Loren B. Andreas, P. K. Madhu, Guido Pintacuda, Vipin Agarwal
Selective 1 H– 1 H Distance Restraints in Fully Protonated Proteins by Very Fast Magic-Angle Spinning Solid-State NMR
published pages: 2399-2405, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.7b00983
The Journal of Physical Chemistry Letters 8/11 2019-06-07
2017 Olivier Saurel, Iordan Iordanov, Guillaume Nars, Pascal Demange, Tanguy Le Marchand, Loren B. Andreas, Guido Pintacuda, Alain Milon
Local and Global Dynamics in Klebsiella pneumoniae Outer Membrane Protein a in Lipid Bilayers Probed at Atomic Resolution
published pages: 1590-1597, ISSN: 0002-7863, DOI: 10.1021/jacs.6b11565
Journal of the American Chemical Society 139/4 2019-06-07
2017 Benjamin Burcher, Kevin J. Sanders, Ladislav Benda, Guido Pintacuda, Erwann Jeanneau, Andreas A. Danopoulos, Pierre Braunstein, Hélène Olivier-Bourbigou, Pierre-Alain R. Breuil
Straightforward Access to Stable, 16-Valence-Electron Phosphine-Stabilized Fe 0 Olefin Complexes and Their Reactivity
published pages: 605-613, ISSN: 0276-7333, DOI: 10.1021/acs.organomet.6b00803
Organometallics 36/3 2019-06-07
2017 Daniela Lalli, Matthew N. Idso, Loren B. Andreas, Sunyia Hussain, Naomi Baxter, Songi Han, Bradley F. Chmelka, Guido Pintacuda
Proton-Based Structural Analysis of a Heptahelical Transmembrane Protein in Lipid Bilayers
published pages: 13006-13012, ISSN: 0002-7863, DOI: 10.1021/jacs.7b05269
Journal of the American Chemical Society 139/37 2019-06-07
2017 Andrew J. Pell, Kevin J. Sanders, Sebastian Wegner, Guido Pintacuda, Clare P. Grey
Low-power broadband solid-state MAS NMR of 14 N
published pages: 194202, ISSN: 0021-9606, DOI: 10.1063/1.4983220
The Journal of Chemical Physics 146/19 2019-06-07

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