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ProNeurons SIGNED

Transcription Factor-mediated Neuronal Cell Fate Programming in Human Stem Cells

Total Cost €

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EC-Contrib. €

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Partnership

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 ProNeurons project word cloud

Explore the words cloud of the ProNeurons project. It provides you a very rough idea of what is the project "ProNeurons" about.

reverse    enigmatic    functional    biology    hence    systematically    conditional    few    full    vitro    underlying    transcriptomic    ethically    approved    stem    morphological    diseases    library    reporter    neurogenesis    unlike    photoreceptors    paves    programming    entire    multifaceted    protocols    lines    expanded    rna    throughput    easily    facilitates    developmental    revealed    setting    retinal    induce    cell    gene    first    neurons    pluripotent    discoveries    rules    confer    anatomical    cells    engineer    potency    modes    brain    ips    human    fluorescent    events    individual    transcription    differentiation    networks    sequencing    dish    biological    genesis    types    engineering    buttons    experiments    generation    period    producing    molecular    discovery    regulatory    reprogramming    transplantation    isolation    characterizations    explore    conceptually    plan    infancy    degeneration    mimic    neuronal    combine    differentiated    vivo    phenomena   

Project "ProNeurons" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITAET DRESDEN 

Organization address
address: HELMHOLTZSTRASSE 10
city: DRESDEN
postcode: 1069
website: http://www.tu-dresden.de/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website https://www.crt-dresden.de/research/research-groups/core-groups/crtd-core-groups/neuronal-cell-types-and-circuit-engineering/neuronal-cell-types-and-circuit-engineering-future-projects-and-goals/
 Total cost 1˙495˙000 €
 EC max contribution 1˙495˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-STG
 Funding Scheme ERC-STG
 Starting year 2016
 Duration (year-month-day) from 2016-03-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAET DRESDEN DE (DRESDEN) coordinator 1˙495˙000.00

Map

 Project objective

The discovery of pluripotent stem cells has expanded the working modes in biology towards the reverse engineering of specific cell types. Unlike studying developmental phenomena in vivo, we are now theoretically able to mimic some of these processes in a dish. The use of human induced pluripotent stem (iPS) cells facilitates studying the genesis of human cell types in an ethically approved setting. However, exploiting the full potency of stem cells is only possible with very few differentiated cell types. In particular, the generation of neurons is in its infancy: of the many neuronal types present in the brain, only a few types have been generated in vitro. So far, neuronal differentiation protocols are multifaceted and tailored to individual cell types. The molecular events that occur during reprogramming remain enigmatic. Hence, we cannot confer these protocols easily on producing different neurons of interest. Therefore, we plan to induce transcription factors as differentiation control buttons in human iPS cells in order to explore in vitro neurogenesis systematically. First, we will apply a human transcription factor library to conditional fluorescent iPS reporter lines, facilitating high-throughput isolation and analysis of induced neurons. Second, the underlying gene regulatory networks will be revealed using RNA-sequencing over the entire differentiation period to identify the biological rules of in vitro neuronal differentiation. We will combine these in-depth transcriptomic analyses with morphological, anatomical, and functional characterizations. Finally, based on our discoveries, we will engineer human photoreceptors that can be applied to cell transplantation experiments in retinal degeneration diseases. Conceptually, our approach paves the way for targeted “forward” programming of human iPS cells to neurons.

 Publications

year authors and title journal last update
List of publications.
2017 Simon D. Klapper, Evelyn J. Sauter, Anka Swiersy, Max A. E. Hyman, Christian Bamann, Ernst Bamberg, Volker Busskamp
On-demand optogenetic activation of human stem-cell-derived neurons
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-017-14827-6
Scientific Reports 7/1 2019-07-08
2016 Simon D. Klapper, Anka Swiersy, Ernst Bamberg, Volker Busskamp
Biophysical Properties of Optogenetic Tools and Their Application for Vision Restoration Approaches
published pages: , ISSN: 1662-5137, DOI: 10.3389/fnsys.2016.00074
Frontiers in Systems Neuroscience 10 2019-07-08
2017 Rebecca S. Lam, Felix M. Töpfer, Phillip G. Wood, Volker Busskamp, Ernst Bamberg
Functional Maturation of Human Stem Cell-Derived Neurons in Long-Term Cultures
published pages: e0169506, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0169506
PLOS ONE 12/1 2019-07-08
2018 Lisa K. Kutsche, Deisy M. Gysi, Joerg Fallmann, Kerstin Lenk, Rebecca Petri, Anka Swiersy, Simon D. Klapper, Karolina Pircs, Shahryar Khattak, Peter F. Stadler, Johan Jakobsson, Katja Nowick, Volker Busskamp
Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
published pages: 438-452.e8, ISSN: 2405-4712, DOI: 10.1016/j.cels.2018.08.011
Cell Systems 7/4 2019-04-18

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