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Development of high-speed microscopes to study wide-scale neural activity

Total Cost €


EC-Contrib. €






 WideBrainImaging project word cloud

Explore the words cloud of the WideBrainImaging project. It provides you a very rough idea of what is the project "WideBrainImaging" about.

multiphoton    otherwise    eeg    tissue    thousands    intact    emerged    consequently    individual    powerful    neuroscience    photon    focal    suppresses    slow    few    relies    neurons    cell    microscope    image    scanning    memory    enlarge    once    regime    completion    opaque    larger    neural    fluorescent    limit    reduces    background    6mm    computation    1mm    volume    relatively    multiple    continue    attempt    imaging    electrodes    insights    limited    opening    enduring    retention    generally    scan    observe    accessible    resolution    cellular    excitation    deeper    spot    volumes    dynamic    transfer    resonant    exceeding    brains    achievable    25x    observation    temporal    ultrafast    inserted    complementary    uses    microscopes    patterns    dynamics    pixels    penetrate    networks    cortex    wavelengths    reveal    provides    single       hippocampus    controllably    functional    sculpt    faster    tool    cells    longer    layers    measured    speed    fluorescence   

Project "WideBrainImaging" data sheet

The following table provides information about the project.


Organization address
city: WIEN
postcode: 1030

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Austria [AT]
 Project website
 Total cost 178˙156 €
 EC max contribution 178˙156 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-03-01   to  2018-02-28


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

The dynamic of neural computation is often studied in individual cells using inserted electrodes, or using low-resolution methods such as EEG. Functional fluorescent imaging has recently emerged as a powerful complementary tool that allows single-cell resolution of relatively large networks, opening a new regime to neuroscience. However, complex brains are generally opaque and can only be studied with scanning two-photon microscopes; with the achievable depth limited to ~0.6mm, and the volume limited by the relatively slow scan. This project will develop ultrafast scanning multiphoton microscopes to image neural activity at cellular resolution over large volumes, and at greater depth. Using these we will study patterns of activity in the hippocampus, and particularly attempt to observe the pathways involved in memory retention. To increase speed we will use temporal focusing to controllably sculpt the excitation volume and enlarge the focal spot. This reduces the number of measured pixels and allows faster scanning (or larger volume), at the cost of resolution. This will allow 25x faster imaging in a resonant scanning two-photon microscope; allowing observation of many thousands of cells at once, which could reveal the wide-scale characteristic activity. We will build a second microscope that uses three-photon excitation with temporal focusing. Three-photon imaging relies on longer wavelengths that penetrate deeper into tissue, and also suppresses background fluorescence which could otherwise limit depth. Consequently, this microscope will allow high-speed imaging at depth exceeding 1mm. This allows study of information transfer across multiple layers; or provides access to the hippocampus through the intact cortex. These studies could provide crucial insights to neuroscience that are currently accessible only for a few neurons. Following completion of this project these microscopes could have an enduring impact as they continue to be used to study neural dynamics.

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The information about "WIDEBRAINIMAGING" are provided by the European Opendata Portal: CORDIS opendata.

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