Opendata, web and dolomites

XPGCS SIGNED

Single cell profiling of X chromosome reactivation during primordial germ cell specification in vivo

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 XPGCS project word cloud

Explore the words cloud of the XPGCS project. It provides you a very rough idea of what is the project "XPGCS" about.

multidisciplinary    subsequent    initiation    embryos    xp    expression    c57bl    f1    inactive    innovative    chromatin    regulation    paternal    female    specification    rate    precursors    explore    eggs    genomic    genders    pgcs    histone    parallel    reprogramming    inactivation    altogether    mapped    model    compensation    xm    xci    h3k27me3    germ    transcriptome    linked    mouse    cell    monitoring    sperm    chromosome    rna    allele    hybrid    erasure    chromosomes    castaneus    insights    pgc    enrichment    coating    undergoes    germline    conjunction    roles    cells    mark    xist    primordial    progressive    inactivated    maternal    extensive    entire    demethylase    polymorphisms    single    coding    biallelic    genetic    xi    gene    transmit    mechanisms    conditional    sequence    unravel    dosage    repressive    perform    global    reactivation    generations    utx    plays    epigenetic    mutant    mice    methylation    followed    dynamics    little    imprints    genes    provides    kinetics    strains   

Project "XPGCS" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.gurdon.cam.ac.uk/research/surani
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-03-01   to  2018-04-17

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 195˙454.00

Map

 Project objective

In female mice, one of the two X chromosomes is inactivated during early development to ensure dosage compensation between genders. A long non-coding gene, Xist, plays a crucial role in the initiation of the X chromosome inactivation (XCI) process. Primordial germ cells (PGCs), the precursors of sperm and eggs, transmit genetic and epigenetic information to subsequent generations, following extensive reprogramming, erasure of methylation and genomic imprints, and X chromosome reactivation. The inactive X (Xi) then undergoes progressive reprogramming and reactivation in the germline of female embryos, through the loss of Xist RNA coating, followed by the erasure of the repressive H3K27me3 histone mark, and eventually biallelic expression of X-linked genes. Although the global dynamics of Xi reactivation have been mapped little is known about the gene-specific dynamics, or the mechanisms involved. To explore gene activity on the entire X chromosome during reprogramming in the germline, I will perform single-cell transcriptome analyses on PGCs from C57BL/6 X Castaneus F1 female hybrid embryos. The high rate of sequence polymorphisms between these strains provides allele-specific information for the activity of the Xp (paternal X) and the Xm (maternal X). I will investigate the kinetics of Xp and Xm gene reactivation chromosome-wide at the single cell level following PGC specification. Furthermore, I will study the dynamics of the expression of X-linked genes in conjunction with chromatin changes in PGCs by monitoring the loss of H3K27me3 enrichment on the inactive X chromosome. In parallel, I will study how the regulation of H3K27me3 affect Xi reactivation by using a conditional mutant mouse model of a H3K27me3 demethylase, UTX. Altogether, the innovative and multidisciplinary approach of this study will unravel important insights on germline reprogramming and the roles of chromatin changes associated with X chromosome reactivation.

 Publications

year authors and title journal last update
List of publications.
2018 Jan J Zylicz, Maud Borensztein, Frederick CK Wong, Yun Huang, Caroline Lee, Sabine Dietmann, M Azim Surani
G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.33361
eLife 7 2019-06-13
2017 Maud Borensztein, Ikuhiro Okamoto, Laurène Syx, Guillaume Guilbaud, Christel Picard, Katia Ancelin, Rafael Galupa, Patricia Diabangouaya, Nicolas Servant, Emmanuel Barillot, Azim Surani, Mitinori Saitou, Chong-Jian Chen, Konstantinos Anastassiadis, Edith Heard
Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-01415-5
Nature Communications 8/1 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "XPGCS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "XPGCS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

CoCoNat (2019)

Coordination in constrained and natural distributed systems

Read More  

ASIQS (2019)

Antiferromagnetic spintronics investigated by quantum sensing techniques

Read More  

PaSION (2018)

A longitudinal assessment of treatment experience, symptoms and potential associations with biomarkers in cancer patients undergoing immune checkpoint inhibitor therapy

Read More