Explore the words cloud of the EpiMIRgen project. It provides you a very rough idea of what is the project "EpiMIRgen" about.
The following table provides information about the project.
ROYAL COLLEGE OF SURGEONS IN IRELAND
|Coordinator Country||Ireland [IE]|
|Total cost||187˙866 €|
|EC max contribution||187˙866 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2016-07-01 to 2018-06-30|
Take a look of project's partnership.
|1||ROYAL COLLEGE OF SURGEONS IN IRELAND||IE (DUBLIN)||coordinator||187˙866.00|
Evidence is emerging that rapid, profound and persisting changes in gene expression regulation and post-transcriptional regulation underlies the epileptogenic process. The current proposal builds on preliminary data which demonstrates that rapid reduction of a microRNA; miR-124, causes increased expression and activity of NRSF, a master-regulator of epileptogenesis. The current proposal will build on this and investigate other gene networks regulated by miR-124 in neurons, by developing the first miR-124 KO mouse using CRISPR technology and a miR-124 overexpressing mouse. These transgenic mice will then be profiled at the epigenomic level using ATAC-Seq, the transcriptome level using HITS-CLIP and ribosomal profiling and the proteomic level using mass spec. This will be the first study to examine the pleiotropic role of miR-124 in mature neurons and identify gene networks regulated by this neuronally enriched miRNA. If miR-124 disruption causes aberrant activity of epigenetic modifiers including NRSF then we will test whether miR-124 restitution can restore correct gene expression networks and prevent or modify epileptogenesis in a mouse model of the disorder. Next we will determine whether data obtained in mouse models is translatable to the human form of the condition by obtaining and maintaining resected human epileptic hippocampus live in culture. We will ectopically introduce miR-124 and test the effect of miR-124 restitution on network activity and energetics using live-calcium imaging as well as the epigenomic and transcriptomic effects. Together this proposal represents the most in-depth analysis of miRNA function and will set the standard for future functional analyses of these molecules. Furthermore it has the potential to intervene in disease process and apply findings to a relevant human model providing a novel therapeutic target for the treatment of epilepsy.
|year||authors and title||journal||last update|
Gary P. Brennan, David C. Henshall
microRNAs in the pathophysiology of epilepsy
published pages: 47-52, ISSN: 0304-3940, DOI: 10.1016/j.neulet.2017.01.017
|Neuroscience Letters 667||2019-06-13|
Tobias Engel, Gary P. Brennan, Amaya Sanz-Rodriguez, Mariana Alves, Edward Beamer, Orla Watters, David C. Henshall, Eva M. Jimenez-Mateos
A calcium-sensitive feed-forward loop regulating the expression of the ATP-gated purinergic P2X7 receptor via specificity protein 1 and microRNA-22
published pages: 255-266, ISSN: 0167-4889, DOI: 10.1016/j.bbamcr.2016.11.007
|Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1864/2||2019-06-13|
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The information about "EPIMIRGEN" are provided by the European Opendata Portal: CORDIS opendata.