Opendata, web and dolomites

TF3C_EM

Structure-function studies of the general transcription factor IIIC (TFIIIC)

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 TF3C_EM project word cloud

Explore the words cloud of the TF3C_EM project. It provides you a very rough idea of what is the project "TF3C_EM" about.

environment    tf3c    tdna    single    ray    structural    molecules    me    spanning    subcomplexes    delivering    unparalleled    shape    rnas    sites    internal    synthesis    rna    termed    linked    exploring    begins    bind    structure    bound    biophysical    dynamics    iiib    pave    gene    chain    first    fret    molecular    genome    disciplinary    interestingly    extra    significantly    transcriptional    transcription    microscopy    assembled    recognition    initiation    function    pol    career    molecule    characterise    roles    characterisation    varying    independent    promoter    form    instrumental    recruitment    tackle    adaptor    iiic    respectively    acid    trnas    correlation    techniques    polymerase    act    translation    peptide    architecture    researcher    underlying    correct    inter    eukaryotes    followed    messenger    electron    transfer    vitro    tau    unravel    time    machinery    scientific    purified    mechanism    pursue    complementing    remodelling    guardian    binding    amino    flexibly    lengths    understand    tfiiic    trna    interactions    crystallography    light    cryo    recombinantly    chromatin    cellular    decoding    box    accommodate    highlights    em   

Project "TF3C_EM" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 159˙460.00

Map

 Project objective

Transfer RNAs (tRNAs) act as adaptor molecules, decoding messenger RNA and delivering the correct amino acid to the growing peptide chain. Being instrumental in the cellular translation machinery, it is important to understand their synthesis mechanism. In eukaryotes, tRNA gene (tDNA) transcription begins with transcription factor IIIC (TFIIIC) bound to 2 gene-internal promoter elements, A- and B-box. This is followed by the recruitment of transcription factor IIIB and RNA polymerase III to form the transcription pre-initiation complex. In ‘TF3C_EM’, I will focus on the mechanism and molecular architecture of the very first step, i.e., TFIIIC binding to tDNA. TFIIIC is composed of two subcomplexes, τA and τB, that bind respectively to the A- and B-box, and are flexibly linked to accommodate varying lengths between their binding sites. Interestingly, TFIIIC has recently been termed as ‘guardian of the genome’ in light of its extra transcriptional roles, such as chromatin remodelling. This further highlights the importance of exploring its underlying structure. Using recombinantly purified TFIIIC, I will characterise the fully assembled TFIIIC complex and TFIIIC-tDNA interactions in vitro. To this end, an inter-disciplinary approach will be used, spanning complementing biophysical and structural techniques of single molecule FRET, cryo electron microscopy and X-ray crystallography. Thus, in my project ‘TF3C_EM’, I will tackle 3 major objectives: (1) characterisation of the molecular architecture of TFIIIC, (2) understanding the tDNA recognition mechanism by TFIIIC and (3) understanding the dynamics between τA and τB. It will unravel the structure-function correlation of TFIIIC and pave the way for further understanding the transcription initiation by Pol III. At the same time, it will allow me to pursue research in an unparalleled scientific environment and significantly contribute to shape my future career as an independent researcher.

 Publications

year authors and title journal last update
List of publications.
2017 Heena Khatter, Matthias K Vorländer, Christoph W Müller
RNA polymerase I and III: similar yet unique
published pages: 88-94, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2017.05.008
Current Opinion in Structural Biology 47 2019-06-13
2018 Matthias K. Vorländer, Heena Khatter, Rene Wetzel, Wim J. H. Hagen, Christoph W. Müller
Molecular mechanism of promoter opening by RNA polymerase III
published pages: 295-300, ISSN: 0028-0836, DOI: 10.1038/nature25440
Nature 553/7688 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TF3C_EM" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TF3C_EM" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MultiSeaSpace (2019)

Developing a unified spatial modelling strategy that accounts for interactions between species at different marine trophic levels, and different types of survey data.

Read More  

MOSAiC (2019)

Multimode cOrrelations in microwave photonics with Superconducting quAntum Circuits

Read More  

POSPORI (2019)

Polymer Optical Sensors for Prolonged Overseeing the Robustness of civil Infrastructures

Read More