Opendata, web and dolomites

TF3C_EM

Structure-function studies of the general transcription factor IIIC (TFIIIC)

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 TF3C_EM project word cloud

Explore the words cloud of the TF3C_EM project. It provides you a very rough idea of what is the project "TF3C_EM" about.

termed    structural    assembled    single    microscopy    tackle    linked    rnas    characterisation    structure    accommodate    biophysical    molecule    pave    internal    chromatin    pol    transcriptional    acid    followed    extra    subcomplexes    light    spanning    scientific    remodelling    electron    form    trnas    tdna    underlying    me    transcription    complementing    career    initiation    machinery    box    correlation    molecules    iiic    dynamics    peptide    understand    eukaryotes    cellular    correct    function    techniques    delivering    recombinantly    chain    recruitment    bound    interestingly    iiib    disciplinary    roles    significantly    sites    lengths    interactions    tf3c    crystallography    trna    inter    pursue    promoter    fret    rna    bind    messenger    architecture    act    instrumental    transfer    guardian    ray    vitro    flexibly    purified    highlights    begins    gene    cryo    polymerase    shape    genome    first    amino    independent    binding    tau    respectively    mechanism    tfiiic    environment    recognition    molecular    characterise    em    adaptor    synthesis    exploring    translation    researcher    time    unparalleled    varying    decoding    unravel   

Project "TF3C_EM" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 159˙460.00

Map

 Project objective

Transfer RNAs (tRNAs) act as adaptor molecules, decoding messenger RNA and delivering the correct amino acid to the growing peptide chain. Being instrumental in the cellular translation machinery, it is important to understand their synthesis mechanism. In eukaryotes, tRNA gene (tDNA) transcription begins with transcription factor IIIC (TFIIIC) bound to 2 gene-internal promoter elements, A- and B-box. This is followed by the recruitment of transcription factor IIIB and RNA polymerase III to form the transcription pre-initiation complex. In ‘TF3C_EM’, I will focus on the mechanism and molecular architecture of the very first step, i.e., TFIIIC binding to tDNA. TFIIIC is composed of two subcomplexes, τA and τB, that bind respectively to the A- and B-box, and are flexibly linked to accommodate varying lengths between their binding sites. Interestingly, TFIIIC has recently been termed as ‘guardian of the genome’ in light of its extra transcriptional roles, such as chromatin remodelling. This further highlights the importance of exploring its underlying structure. Using recombinantly purified TFIIIC, I will characterise the fully assembled TFIIIC complex and TFIIIC-tDNA interactions in vitro. To this end, an inter-disciplinary approach will be used, spanning complementing biophysical and structural techniques of single molecule FRET, cryo electron microscopy and X-ray crystallography. Thus, in my project ‘TF3C_EM’, I will tackle 3 major objectives: (1) characterisation of the molecular architecture of TFIIIC, (2) understanding the tDNA recognition mechanism by TFIIIC and (3) understanding the dynamics between τA and τB. It will unravel the structure-function correlation of TFIIIC and pave the way for further understanding the transcription initiation by Pol III. At the same time, it will allow me to pursue research in an unparalleled scientific environment and significantly contribute to shape my future career as an independent researcher.

 Publications

year authors and title journal last update
List of publications.
2017 Heena Khatter, Matthias K Vorländer, Christoph W Müller
RNA polymerase I and III: similar yet unique
published pages: 88-94, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2017.05.008
Current Opinion in Structural Biology 47 2019-06-13
2018 Matthias K. Vorländer, Heena Khatter, Rene Wetzel, Wim J. H. Hagen, Christoph W. Müller
Molecular mechanism of promoter opening by RNA polymerase III
published pages: 295-300, ISSN: 0028-0836, DOI: 10.1038/nature25440
Nature 553/7688 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TF3C_EM" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TF3C_EM" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MingleIFT (2020)

Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

Read More  

MIGPSC (2018)

Shaping the European Migration Policy: the role of the security industry

Read More  

MathematicsAnalogies (2019)

Mathematics Analogies

Read More