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Structure-function studies of the general transcription factor IIIC (TFIIIC)

Total Cost €


EC-Contrib. €






 TF3C_EM project word cloud

Explore the words cloud of the TF3C_EM project. It provides you a very rough idea of what is the project "TF3C_EM" about.

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Project "TF3C_EM" data sheet

The following table provides information about the project.


Organization address
address: Meyerhofstrasse 1
postcode: 69117

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Transfer RNAs (tRNAs) act as adaptor molecules, decoding messenger RNA and delivering the correct amino acid to the growing peptide chain. Being instrumental in the cellular translation machinery, it is important to understand their synthesis mechanism. In eukaryotes, tRNA gene (tDNA) transcription begins with transcription factor IIIC (TFIIIC) bound to 2 gene-internal promoter elements, A- and B-box. This is followed by the recruitment of transcription factor IIIB and RNA polymerase III to form the transcription pre-initiation complex. In ‘TF3C_EM’, I will focus on the mechanism and molecular architecture of the very first step, i.e., TFIIIC binding to tDNA. TFIIIC is composed of two subcomplexes, τA and τB, that bind respectively to the A- and B-box, and are flexibly linked to accommodate varying lengths between their binding sites. Interestingly, TFIIIC has recently been termed as ‘guardian of the genome’ in light of its extra transcriptional roles, such as chromatin remodelling. This further highlights the importance of exploring its underlying structure. Using recombinantly purified TFIIIC, I will characterise the fully assembled TFIIIC complex and TFIIIC-tDNA interactions in vitro. To this end, an inter-disciplinary approach will be used, spanning complementing biophysical and structural techniques of single molecule FRET, cryo electron microscopy and X-ray crystallography. Thus, in my project ‘TF3C_EM’, I will tackle 3 major objectives: (1) characterisation of the molecular architecture of TFIIIC, (2) understanding the tDNA recognition mechanism by TFIIIC and (3) understanding the dynamics between τA and τB. It will unravel the structure-function correlation of TFIIIC and pave the way for further understanding the transcription initiation by Pol III. At the same time, it will allow me to pursue research in an unparalleled scientific environment and significantly contribute to shape my future career as an independent researcher.


year authors and title journal last update
List of publications.
2017 Heena Khatter, Matthias K Vorländer, Christoph W Müller
RNA polymerase I and III: similar yet unique
published pages: 88-94, ISSN: 0959-440X, DOI: 10.1016/
Current Opinion in Structural Biology 47 2019-06-13
2018 Matthias K. Vorländer, Heena Khatter, Rene Wetzel, Wim J. H. Hagen, Christoph W. Müller
Molecular mechanism of promoter opening by RNA polymerase III
published pages: 295-300, ISSN: 0028-0836, DOI: 10.1038/nature25440
Nature 553/7688 2019-06-13

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